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. 1975 Oct;2(10):1851–1865. doi: 10.1093/nar/2.10.1851

Sequences spanning the EcoRI substrate site.

D E Garfin, H W Boyer, H M Goodman
PMCID: PMC343552  PMID: 171627

Abstract

Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the EcoRI site of simian virus 40 DNA and a 15-base-pair sequence overlapping the EcoRI site of Col El plasmid DNA. Three other DNAs were similarly tested, although extended sequences were not determined in these cases. The EcoRI site was shown to be symmetric double-stranded equivalent of -N-G-A-A-T-T-C-N-.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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