Abstract
Equilibrium dialysis, relaxation kinetic, melting, and continuous variation mixing experiments on complexes of poly(dA) and poly(rU) demonstrate that ethidium induces conversion of a 1:1 mixture of these homopolymers (at one molar salt and 19 degrees C) from a three stranded to a two stranded helix. This is the first demonstration of a double helix of poly(dA)-poly(rU) in solution.
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- Bresloff J. L., Crothers D. M. DNA-ethidium reaction kinetics: demonstration of direct ligand transfer between DNA binding sites. J Mol Biol. 1975 Jun 15;95(1):103–123. doi: 10.1016/0022-2836(75)90339-3. [DOI] [PubMed] [Google Scholar]
- Crothers D. M. Calculation of binding isotherms for heterogenous polymers. Biopolymers. 1968 Apr;6(4):575–584. doi: 10.1002/bip.1968.360060411. [DOI] [PubMed] [Google Scholar]
- Crothers D. M. Statistical thermodynamics of nucleic acid melting transitions with coupled binding equilibria. Biopolymers. 1971 Nov;10(11):2147–2160. doi: 10.1002/bip.360101110. [DOI] [PubMed] [Google Scholar]
- INMAN R. B. MULTISTRANDED DNA HOMOPOLYMER INTERACTIONS. J Mol Biol. 1964 Oct;10:137–146. doi: 10.1016/s0022-2836(64)80033-4. [DOI] [PubMed] [Google Scholar]
- Krakauer H., Sturtevant J. M. Heats of the helix-coil transitions of the poly A-poly U complexes. Biopolymers. 1968 Apr;6(4):491–512. doi: 10.1002/bip.1968.360060406. [DOI] [PubMed] [Google Scholar]
- Le Pecq J. B., Paoletti C. Interaction du bromhydrate d'éthidium (BET) avec les polyribonucléotides. Applications à l'étude des réactions d'hybridation. C R Acad Sci Hebd Seances Acad Sci D. 1965 Jun 28;260(26):7033–7036. [PubMed] [Google Scholar]
- Pohl F. M., Jovin T. M., Baehr W., Holbrook J. J. Ethidium bromide as a cooperative effector of a DNA structure. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3805–3809. doi: 10.1073/pnas.69.12.3805. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riley M., Maling B. Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes. J Mol Biol. 1966 Sep;20(2):359–389. doi: 10.1016/0022-2836(66)90069-6. [DOI] [PubMed] [Google Scholar]
- Tritton T. R., Mohr S. C. Kinetics of ethidium bromide binding as a probe of transfer ribonucleic acid structure. Biochemistry. 1973 Feb 27;12(5):905–914. doi: 10.1021/bi00729a018. [DOI] [PubMed] [Google Scholar]
- Waring M. J. Stablilzation of two-standard ribohomopolymer helices and destabilzation of a three-stranded helix by ethidium bromide. Biochem J. 1974 Nov;143(2):483–486. doi: 10.1042/bj1430483. [DOI] [PMC free article] [PubMed] [Google Scholar]