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. 1984 Jan;81(1):189–192. doi: 10.1073/pnas.81.1.189

Reaction center and light-harvesting I genes from Rhodopseudomonas capsulata

Douglas C Youvan *,†, Marie Alberti *, Helmut Begusch *,‡, Edward J Bylina *,‡, John E Hearst *,‡
PMCID: PMC344636  PMID: 16593406

Abstract

Five structural genes coding for the reaction center (RC) L, M, and H subunits and the two light-harvesting (LH) I polypeptides, B870α and B870β, have been mapped on two restriction fragments from the R-prime plasmid pRPS404. It has been recently shown that enhanced near-infrared fluorescence mutants of Rhodopseudomonas capsulata typically lack RC or LH I polypeptides and that these lesions are marker-rescued by two restriction fragments from the R-prime plasmid: the 7.5-kilobase-pair EcoRI F fragment and the 4.75-kilobase-pair BamHI C-EcoRI fragment. We have now determined the nucleotide sequence of two restriction fragments and have found that the BamHI C-EcoRI B fragment carries the structural genes for the RC L and M subunits and both LH I polypeptides. Forty kilobase pairs away from this locus, the BamHI F fragment (within the EcoRI F fragment) carries the RC H subunit. The structural genes on the BamHI C-EcoRI B fragment are probably transcribed as part of a polycistronic mRNA. All of the structural genes begin with a consensus Shine-Dalgarno sequence and separate AUG start codons, indicating that the structural polypeptides are not cleaved from larger precursor polypeptides.

Keywords: photosynthesis, DNA sequence, enhanced fluorescence mutants, R-prime plasmid, genetic map

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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