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. 1984 Feb;81(4):988–992. doi: 10.1073/pnas.81.4.988

Induction of two genes by glucose starvation in hamster fibroblasts.

A Y Lin, A S Lee
PMCID: PMC344748  PMID: 6583707

Abstract

The coordinated expression of two genes specifically induced by glucose starvation is demonstrated in a hamster fibroblast cell line, K12. Using two cDNA plasmids, p4A3 and p3C5, as hybridization probes, we examine the kinetics of induction of these genes when the cells are grown in medium deprived of glucose. The results show that (i) after a lag period of about 8 hr, there is a rapid and simultaneous increase of the p4A3 and p3C5 mRNA levels and (ii) the elevation of the mRNA levels for p4A3 and p3C5 is largely due to new transcription. In addition, we compare the mRNA transcripts encoded by these glucose-regulated genes in culture cells and phosphoenolpyruvate carboxykinase, the enzyme that catalyzes the rate-limiting step in gluconeogenesis in fasted rats. Our results indicate that the expression of phosphoenolpyruvate carboxykinase is not inducible by glucose starvation in our culture cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Deschatrette J., Weiss M. C. Characterization of differentiated and dedifferentiated clones from a rat hepatoma. Biochimie. 1974;56(11-12):1603–1611. doi: 10.1016/s0300-9084(75)80286-0. [DOI] [PubMed] [Google Scholar]
  2. Gunn J. M., Tilghman S. M., Hanson R. W., Reshef L., Ballard F. J. Effects of cyclic adenosine monophosphate, dexamethasone and insulin on phosphoenolpyruvate carboxykinase synthesis in Reuber H-35 hepatoma cells. Biochemistry. 1975 Jun 3;14(11):2350–2357. doi: 10.1021/bi00682a012. [DOI] [PubMed] [Google Scholar]
  3. Hightower L. E. Cultured animal cells exposed to amino acid analogues or puromycin rapidly synthesize several polypeptides. J Cell Physiol. 1980 Mar;102(3):407–427. doi: 10.1002/jcp.1041020315. [DOI] [PubMed] [Google Scholar]
  4. Isaka T., Yoshida M., Owada M., Toyoshima K. Alterations in membrane polypeptides of chick embryo fibroblasts induced by transformation with avian sarcoma viruses. Virology. 1975 May;65(1):226–237. doi: 10.1016/0042-6822(75)90023-9. [DOI] [PubMed] [Google Scholar]
  5. Kelley P. M., Schlesinger M. J. Antibodies to two major chicken heat shock proteins cross-react with similar proteins in widely divergent species. Mol Cell Biol. 1982 Mar;2(3):267–274. doi: 10.1128/mcb.2.3.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lamers W. H., Hanson R. W., Meisner H. M. cAMP stimulates transcription of the gene for cytosolic phosphoenolpyruvate carboxykinase in rat liver nuclei. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5137–5141. doi: 10.1073/pnas.79.17.5137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lee A. S., Delegeane A. M., Baker V., Chow P. C. Transcriptional regulation of two genes specifically induced by glucose starvation in a hamster mutant fibroblast cell line. J Biol Chem. 1983 Jan 10;258(1):597–603. [PubMed] [Google Scholar]
  8. Lee A. S., Delegeane A., Scharff D. Highly conserved glucose-regulated protein in hamster and chicken cells: preliminary characterization of its cDNA clone. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4922–4925. doi: 10.1073/pnas.78.8.4922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee A. S. The accumulation of three specific proteins related to glucose-regulated proteins in a temperature-sensitive hamster mutant cell line K12. J Cell Physiol. 1981 Jan;106(1):119–125. doi: 10.1002/jcp.1041060113. [DOI] [PubMed] [Google Scholar]
  10. McCormick P. J., Keys B. J., Pucci C., Millis A. J. Human fibroblast-conditioned medium contains a 100K dalton glucose-regulated cell surface protein. Cell. 1979 Sep;18(1):173–182. doi: 10.1016/0092-8674(79)90366-0. [DOI] [PubMed] [Google Scholar]
  11. Melero J. A. Identification of the glucose/glycosylation-regulated proteins as those which accumulate in the temperature-sensitive cell line K12. J Cell Physiol. 1981 Oct;109(1):59–67. doi: 10.1002/jcp.1041090108. [DOI] [PubMed] [Google Scholar]
  12. Pellicer A., Wigler M., Axel R., Silverstein S. The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell. 1978 May;14(1):133–141. doi: 10.1016/0092-8674(78)90308-2. [DOI] [PubMed] [Google Scholar]
  13. Pouysségur J., Yamada K. M. Isolation and immunological characterization of a glucose-regulated fibroblast cell surface glycoprotein and its nonglycosylated precursor. Cell. 1978 Jan;13(1):139–140. doi: 10.1016/0092-8674(78)90145-9. [DOI] [PubMed] [Google Scholar]
  14. Rognstad R. Rate-limiting steps in metabolic pathways. J Biol Chem. 1979 Mar 25;254(6):1875–1878. [PubMed] [Google Scholar]
  15. Shiu R. P., Pouyssegur J., Pastan I. Glucose depletion accounts for the induction of two transformation-sensitive membrane proteinsin Rous sarcoma virus-transformed chick embryo fibroblasts. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3840–3844. doi: 10.1073/pnas.74.9.3840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Stone K. R., Smith R. E., Joklik W. K. Changes in membrane polypeptides that occur when chick embryo fibroblasts and NRK cells are transformed with avian sarcoma viruses. Virology. 1974 Mar;58(1):86–100. doi: 10.1016/0042-6822(74)90143-3. [DOI] [PubMed] [Google Scholar]
  18. Wang M. L., Lee A. S. Polymerization of vector DNA after transfection into hamster fibroblast cells. Biochem Biophys Res Commun. 1983 Jan 27;110(2):593–601. doi: 10.1016/0006-291x(83)91191-9. [DOI] [PubMed] [Google Scholar]
  19. Yoo-Warren H., Monahan J. E., Short J., Short H., Bruzel A., Wynshaw-Boris A., Meisner H. M., Samols D., Hanson R. W. Isolation and characterization of the gene coding for cytosolic phosphoenolpyruvate carboxykinase (GTP) from the rat. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3656–3660. doi: 10.1073/pnas.80.12.3656. [DOI] [PMC free article] [PubMed] [Google Scholar]

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