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. 1984 Feb;81(4):1045–1047. doi: 10.1073/pnas.81.4.1045

Biologic activity in a fragment of recombinant human interferon alpha.

S K Ackerman, D Zur Nedden, M Heintzelman, M Hunkapiller, K Zoon
PMCID: PMC344760  PMID: 6199790

Abstract

To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-alpha 2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-alpha 2-(1-110) and HuIFN-alpha 2-(111-153). After reduction with 2-mercaptoethanol and separation of the two major fragments on NaDodSO4/polyacrylamide gel electrophoresis, antiviral activity persisted in the larger, Mr 12,000, fragment consisting of the amino-terminal 110 amino acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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