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. 1984 Mar;81(5):1411–1415. doi: 10.1073/pnas.81.5.1411

Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.

R Enat, D M Jefferson, N Ruiz-Opazo, Z Gatmaitan, L A Leinwand, L M Reid
PMCID: PMC344845  PMID: 6584889

Abstract

The culture conditions found to result in stable proliferation of normal rat hepatocytes are: (i) subconfluent cell densities; (ii) serum-free medium; (iii) hormonally defined medium containing epidermal growth factor, insulin, glucagon, prolactin, and other growth factors; and (iv) substrata of liver extracellular matrix depleted of growth inhibitors. Serum was found deleterious to parenchymal cells: it was inhibitory to the expression of liver-specific functions, cytostatic to parenchymal cells at all seeding densities, and cytotoxic to them at low seeding densities. These studies emphasize the relevance of synergies in the influences of hormones and extracellular matrix in regulating hepatocellular physiology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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