Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Feb;81(3):699–703. doi: 10.1073/pnas.81.3.699

Major coat protein and single-stranded DNA-binding protein of filamentous virus Pf3.

D G Putterman, A Casadevall, P D Boyle, H L Yang, B Frangione, L A Day
PMCID: PMC344902  PMID: 6422463

Abstract

The region of the Pf3 virus genome encoding its major coat protein and its single-stranded DNA-binding protein is organized somewhat like the corresponding region of the fd (M13, f1) genome. Nevertheless, the major coat protein is unique among the major coat proteins of fd and the other filamentous phages studied in that it lacks a signal sequence and appears to be a direct translation product and in that it has fewer basic amino acid residues than its equivalent of DNA phosphates in the virion. These features are relevant to considerations of both protein insertion into membranes and DNA structure in filamentous viruses. The single-stranded DNA-binding protein also has a sequence that is different from the sequences of single-stranded DNA-binding proteins from other filamentous viruses.

Full text

PDF
702

Images in this article

700Image
on p.700
700Image
on p.700

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts B., Frey L., Delius H. Isolation and characterization of gene 5 protein of filamentous bacterial viruses. J Mol Biol. 1972 Jul 14;68(1):139–152. doi: 10.1016/0022-2836(72)90269-0. [DOI] [PubMed] [Google Scholar]
  2. Bailey G. S., Gillett D., Hill D. F., Petersen G. B. Automated sequencing of insoluble peptides using detergent. Bacteriophage fl coat protein. J Biol Chem. 1977 Apr 10;252(7):2218–2225. [PubMed] [Google Scholar]
  3. Beck E., Sommer R., Auerswald E. A., Kurz C., Zink B., Osterburg G., Schaller H., Sugimoto K., Sugisaki H., Okamoto T. Nucleotide sequence of bacteriophage fd DNA. Nucleic Acids Res. 1978 Dec;5(12):4495–4503. doi: 10.1093/nar/5.12.4495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beck E., Zink B. Nucleotide sequence and genome organisation of filamentous bacteriophages fl and fd. Gene. 1981 Dec;16(1-3):35–58. doi: 10.1016/0378-1119(81)90059-7. [DOI] [PubMed] [Google Scholar]
  5. Berkowitz S. A., Day L. A. Turbidity measurements in an analytical ultracentrifuge. Determinations of mass per length for filamentous viruses fd, Xf, and Pf3. Biochemistry. 1980 Jun 10;19(12):2696–2702. doi: 10.1021/bi00553a025. [DOI] [PubMed] [Google Scholar]
  6. Blobel G., Dobberstein B. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 1975 Dec;67(3):835–851. doi: 10.1083/jcb.67.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casadevall A., Day L. A. DNA packing in the filamentous viruses fd, Xf, Pf1 and Pf3. Nucleic Acids Res. 1982 Apr 10;10(7):2467–2481. doi: 10.1093/nar/10.7.2467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Casadevall A., Day L. A. Silver and mercury probing of deoxyribonucleic acid structures in the filamentous viruses fd, If1, IKe, Xf, Pf1, and Pf3. Biochemistry. 1983 Sep 27;22(20):4831–4842. doi: 10.1021/bi00289a033. [DOI] [PubMed] [Google Scholar]
  9. Chang C. N., Blobel G., Model P. Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein. Proc Natl Acad Sci U S A. 1978 Jan;75(1):361–365. doi: 10.1073/pnas.75.1.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cross T. A., Tsang P., Opella S. J. Comparison of protein and deoxyribonucleic acid backbone structures in fd and Pf1 bacteriophages. Biochemistry. 1983 Feb 15;22(4):721–726. doi: 10.1021/bi00273a002. [DOI] [PubMed] [Google Scholar]
  11. Cuypers T., van der Ouderaa F. J., de Jong W. W. The amino acid sequence of gene 5 protein of bacteriophage M 13. Biochem Biophys Res Commun. 1974 Jul 24;59(2):557–563. doi: 10.1016/s0006-291x(74)80016-1. [DOI] [PubMed] [Google Scholar]
  12. Day L. A., Wiseman R. L., Marzec C. J. Structure models for DNA in filamentous viruses with phosphates near the center. Nucleic Acids Res. 1979 Nov 24;7(6):1393–1403. doi: 10.1093/nar/7.6.1393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Doolittle R. F. Similar amino acid sequences: chance or common ancestry? Science. 1981 Oct 9;214(4517):149–159. doi: 10.1126/science.7280687. [DOI] [PubMed] [Google Scholar]
  14. Edens L., Konings R. N., Schoenmakers J. G. A cascade mechanism of transcription in bacteriophage M13 DNA. Virology. 1978 May 15;86(2):354–367. doi: 10.1016/0042-6822(78)90076-4. [DOI] [PubMed] [Google Scholar]
  15. Emr S. D., Hall M. N., Silhavy T. J. A mechanism of protein localization: the signal hypothesis and bacteria. J Cell Biol. 1980 Sep;86(3):701–711. doi: 10.1083/jcb.86.3.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Frangione B., Milstein C. Variations in the S-S bridges of immunoglobins G: interchain disulfide bridges of gamma G3 myeloma proteins. J Mol Biol. 1968 May 14;33(3):893–906. doi: 10.1016/0022-2836(68)90326-4. [DOI] [PubMed] [Google Scholar]
  17. Frangione B., Nakashima Y., Konigsberg W., Wiseman R. L. The amino acid sequence of the major coat protein subunit of the filamentous virus Xf. FEBS Lett. 1978 Dec 15;96(2):381–384. doi: 10.1016/0014-5793(78)80442-6. [DOI] [PubMed] [Google Scholar]
  18. Frangione B., Rosenwasser E., Prelli F., Franklin E. C. Primary structure of human gamma 3 immunoglobulin deletion mutant: gamma 3 heavy-chain disease protein Wis. Biochemistry. 1980 Sep 2;19(18):4304–4308. doi: 10.1021/bi00559a024. [DOI] [PubMed] [Google Scholar]
  19. Gray C. W., Kneale G. G., Leonard K. R., Siegrist H., Marvin D. A. A nucleoprotein complex in bacteria infected with PF1 filamentous virus: identification and electron microscopic analysis. Virology. 1982 Jan 15;116(1):40–52. doi: 10.1016/0042-6822(82)90401-9. [DOI] [PubMed] [Google Scholar]
  20. Hill D. F., Petersen G. B. Nucleotide sequence of bacteriophage f1 DNA. J Virol. 1982 Oct;44(1):32–46. doi: 10.1128/jvi.44.1.32-46.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ito K., Mandel G., Wickner W. Soluble precursor of an integral membrane protein: synthesis of procoat protein in Escherichia coli infected with bacteriophage M13. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1199–1203. doi: 10.1073/pnas.76.3.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Luiten R. G., Schoenmakers J. G., Konings R. N. The major coat protein gene of the filamentous Pseudomonas aeruginosa phage Pf3: absence of an N-terminal leader signal sequence. Nucleic Acids Res. 1983 Nov 25;11(22):8073–8085. doi: 10.1093/nar/11.22.8073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Maeda K., Kneale G. G., Tsugita A., Short N. J., Perham R. N., Hill D. F., Petersen G. B. The DNA-binding protein of Pf1 filamentous bacteriophage: amino-acid sequence and structure of the gene. EMBO J. 1982;1(2):255–261. doi: 10.1002/j.1460-2075.1982.tb01156.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Marzec C. J., Day L. A. DNA and protein lattice-lattice interactions in the filamentous bacteriophages. Biophys J. 1983 May;42(2):171–180. doi: 10.1016/S0006-3495(83)84383-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Michaelsen T. E., Frangione B., Franklin E. C. Primary structure of the "hinge" region of human IgG3. Probable quadruplication of a 15-amino acid residue basic unit. J Biol Chem. 1977 Feb 10;252(3):883–889. [PubMed] [Google Scholar]
  27. Nakashima Y., Frangione B., Wiseman R. L., Konigsberg W. H. Primary structure of the major coat protein of the filamentous bacterial viruses, If1 and Ike. J Biol Chem. 1981 Jun 10;256(11):5792–5797. [PubMed] [Google Scholar]
  28. Nakashima Y., Konigsberg W. Reinvestigation of a region of the fd bacteriophage coat protein sequence. J Mol Biol. 1974 Sep 25;88(3):598–600. doi: 10.1016/0022-2836(74)90410-0. [DOI] [PubMed] [Google Scholar]
  29. Nakashima Y., Wiseman R. L., Konigsberg W., Marvin D. A. Primary structure and sidechain interactions of PFL filamentous bacterial virus coat protein. Nature. 1975 Jan 3;253(5486):68–71. doi: 10.1038/253068a0. [DOI] [PubMed] [Google Scholar]
  30. Newman J., Day L. A., Dalack G. W., Eden D. Hydrodynamic determination of molecular weight, dimensions, and structural parameters of Pf3 virus. Biochemistry. 1982 Jul 6;21(14):3352–3358. doi: 10.1021/bi00257a016. [DOI] [PubMed] [Google Scholar]
  31. Okamoto T., Sugiura M., Takanami M. Length of RNA transcribed on the replicative form DNA of coliphage fd. J Mol Biol. 1969 Oct 14;45(1):101–111. doi: 10.1016/0022-2836(69)90213-7. [DOI] [PubMed] [Google Scholar]
  32. Peeters B. P., Konings R. N., Schoenmakers J. G. Characterization of the DNA binding protein encoded by the N-specific filamentous Escherichia coli phage IKe. Binding properties of the protein and nucleotide sequence of the gene. J Mol Biol. 1983 Sep 5;169(1):197–215. doi: 10.1016/s0022-2836(83)80180-6. [DOI] [PubMed] [Google Scholar]
  33. Pratt D., Laws P., Griffith J. Complex of bacteriophage M13 single-stranded DNA and gene 5 protein. J Mol Biol. 1974 Feb 5;82(4):425–439. doi: 10.1016/0022-2836(74)90239-3. [DOI] [PubMed] [Google Scholar]
  34. Pribnow D. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci U S A. 1975 Mar;72(3):784–788. doi: 10.1073/pnas.72.3.784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Putterman D. G., Gryczan T. J., Dubnau D., Day L. A. Cloning of Pf3, a filamentous bacteriophage of Pseudomonas aeruginosa, into the pBD214 vector of Bacillus subtilis. J Virol. 1983 Jul;47(1):221–223. doi: 10.1128/jvi.47.1.221-223.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Russel M., Model P. A mutation downstream from the signal peptidase cleavage site affects cleavage but not membrane insertion of phage coat protein. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1717–1721. doi: 10.1073/pnas.78.3.1717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Seeburg P. H., Schaller H. Mapping and characterization of promoters in bacteriophages fd, f1 and m13. J Mol Biol. 1975 Feb 25;92(2):261–277. doi: 10.1016/0022-2836(75)90226-0. [DOI] [PubMed] [Google Scholar]
  38. Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
  39. Snell D. T., Offord R. E. The amino acid sequence of the B-protein of bacteriophage ZJ-2. Biochem J. 1972 Mar;127(1):167–178. doi: 10.1042/bj1270167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Stanisich V. A. The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. J Gen Microbiol. 1974 Oct;84(2):332–342. doi: 10.1099/00221287-84-2-332. [DOI] [PubMed] [Google Scholar]
  41. Sugimoto K., Sugisaki H., Okamoto T., Takanami M. Studies on bacteriophage fd DNA. IV. The sequence of messenger RNA for the major coat protein gene. J Mol Biol. 1977 Apr 25;111(4):487–507. doi: 10.1016/s0022-2836(77)80065-x. [DOI] [PubMed] [Google Scholar]
  42. Takanami M., Sugimoto K., Sugisaki H., Okamoto T. Sequence of promoter for coat protein gene of bacteriophage fd. Nature. 1976 Mar 25;260(5549):297–302. doi: 10.1038/260297a0. [DOI] [PubMed] [Google Scholar]
  43. Thomas G. J., Jr, Prescott B., Day L. A. Structure similarity, difference and variability in the filamentous viruses fd, If1, IKe, Pf1 and Xf. Investigation by laser Raman spectroscopy. J Mol Biol. 1983 Apr 5;165(2):321–356. doi: 10.1016/s0022-2836(83)80260-5. [DOI] [PubMed] [Google Scholar]
  44. Wickner W. Assembly of proteins into membranes. Science. 1980 Nov 21;210(4472):861–868. doi: 10.1126/science.7001628. [DOI] [PubMed] [Google Scholar]
  45. Yang H. L., Ivashkiv L., Chen H. Z., Zubay G., Cashel M. Cell-free coupled transcription-translation system for investigation of linear DNA segments. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7029–7033. doi: 10.1073/pnas.77.12.7029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. van Wezenbeek P. M., Hulsebos T. J., Schoenmakers J. G. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene. 1980 Oct;11(1-2):129–148. doi: 10.1016/0378-1119(80)90093-1. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES