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. 1984 Feb;81(3):867–870. doi: 10.1073/pnas.81.3.867

Allelic inclusion in the pre-B-cell line 18-81.

M R Wabl, G B Beck-Engeser, P D Burrows
PMCID: PMC344939  PMID: 6322175

Abstract

In an Abelson-virus-transformed mouse lymphoid cell line with pre-B-cell characteristics, a few cells continuously produce heavy chains from both homologs. Each chain has a different variable region. These cells thereby exhibit allelic inclusion rather than allelic exclusion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alt F. W., Rosenberg N., Casanova R. J., Thomas E., Baltimore D. Immunoglobulin heavy-chain expression and class switching in a murine leukaemia cell line. Nature. 1982 Mar 25;296(5855):325–331. doi: 10.1038/296325a0. [DOI] [PubMed] [Google Scholar]
  2. Alt F. W., Rosenberg N., Enea V., Siden E., Baltimore D. Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines. Mol Cell Biol. 1982 Apr;2(4):386–400. doi: 10.1128/mcb.2.4.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buckland R. A., Fletcher J. M., Chandley C. Characterization of the domestic horse (Equus caballus) karyotype using G- and C-banding techniques. Experientia. 1976 Sep 15;32(9):1146–1149. doi: 10.1007/BF01927593. [DOI] [PubMed] [Google Scholar]
  4. Burrows P. D., Beck-Engeser G. B., Wabl M. R. Immunoglobulin heavy-chain class switching in a pre-B cell line is accompanied by DNA rearrangement. Nature. 1983 Nov 17;306(5940):243–246. doi: 10.1038/306243a0. [DOI] [PubMed] [Google Scholar]
  5. Burrows P. D., Beck G. B., Wabl M. R. Expression of mu and gamma immunoglobulin heavy chains in different cells of a cloned mouse lymphoid line. Proc Natl Acad Sci U S A. 1981 Jan;78(1):564–568. doi: 10.1073/pnas.78.1.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burrows P., LeJeune M., Kearney J. F. Evidence that murine pre-B cells synthesise mu heavy chains but no light chains. Nature. 1979 Aug 30;280(5725):838–840. doi: 10.1038/280838a0. [DOI] [PubMed] [Google Scholar]
  7. Gross-Bellard M., Oudet P., Chambon P. Isolation of high-molecular-weight DNA from mammalian cells. Eur J Biochem. 1973 Jul 2;36(1):32–38. doi: 10.1111/j.1432-1033.1973.tb02881.x. [DOI] [PubMed] [Google Scholar]
  8. Haas I. G., Wabl M. Immunoglobulin heavy chain binding protein. Nature. 1983 Nov 24;306(5941):387–389. doi: 10.1038/306387a0. [DOI] [PubMed] [Google Scholar]
  9. Hengartner H., Meo T., Müller E. Assignment of genes for immunoglobulin kappa and heavy chains to chromosomes 6 and 12 in mouse. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4494–4498. doi: 10.1073/pnas.75.9.4494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kearney J. F., Lawton A. R. B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes. J Immunol. 1975 Sep;115(3):671–676. [PubMed] [Google Scholar]
  11. Kloppel T. M., Kubo R. T., Cain P. S., Browne C. P., Colon S. M., Kearney J. F., Grey H. M. Structural analysis of the mu-chains synthesized by fetal liver hybridomas. J Immunol. 1981 Apr;126(4):1346–1350. [PubMed] [Google Scholar]
  12. Maki R., Kearney J., Paige C., Tonegawa S. Immunoglobulin gene rearrangement in immature B cells. Science. 1980 Sep 19;209(4463):1366–1369. doi: 10.1126/science.6774416. [DOI] [PubMed] [Google Scholar]
  13. Meo T., Johnson J., Beechey C. V., Andrews S. J., Peters J., Searle A. G. Linkage analyses of murine immunoglobulin heavy chain and serum prealbumin genes establish their location on chromosome 12 proximal to the T (5;12) 31H breakpoint in band 12F1. Proc Natl Acad Sci U S A. 1980 Jan;77(1):550–553. doi: 10.1073/pnas.77.1.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nesbitt M. N., Francke U. A system of nomenclature for band patterns of mouse chromosomes. Chromosoma. 1973;41(2):145–158. doi: 10.1007/BF00319691. [DOI] [PubMed] [Google Scholar]
  15. Pernis B., Chiappino G., Kelus A. S., Gell P. G. Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J Exp Med. 1965 Nov 1;122(5):853–876. doi: 10.1084/jem.122.5.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Wabl M., Steinberg C. A theory of allelic and isotypic exclusion for immunoglobulin genes. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6976–6978. doi: 10.1073/pnas.79.22.6976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weiler E. Differential activity of allelic gamma-globulin genes in antibody-producing cells. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1765–1772. doi: 10.1073/pnas.54.6.1765. [DOI] [PMC free article] [PubMed] [Google Scholar]

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