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. 1984 Feb;81(3):898–902. doi: 10.1073/pnas.81.3.898

Liver disease associated with duck hepatitis B virus infection of domestic ducks.

P L Marion, S S Knight, B K Ho, Y Y Guo, W S Robinson, H Popper
PMCID: PMC344946  PMID: 6583686

Abstract

The liver disease associated with duck hepatitis B viremia was investigated in naturally infected ducks from Chi-tung county in China and in both naturally and experimentally infected ducks from the United States. Liver and serum specimens of adult Chinese ducks were examined for duck hepatitis B virus (DHBV) DNA by dot and gel blot hybridization. DHBV was found in serum and (in episomal form only) in livers of 6 of 11 birds exhibiting various degrees of chronic hepatitis. In 1 bird with hepatocellular carcinoma, DHBV DNA was detected at the limit of assay sensitivity and in another not at all, contrasting with findings in humans and woodchucks. In work with California Pekin and Khaki Campbell ducks, known amounts of DHBV were injected into the egg 10 days before, or into ducklings 1 day after, hatching and the livers were examined 6 weeks later. The majority of the injected ducklings had viremia detectable by hybridization 1 or 2 weeks after injection. The presence but not the amount of viremia correlated with incidence and degree of hepatitis, determined under code. The most severe instances of hepatitis, all in Pekin ducks, resembled the hepatitis in adult Chinese ducks of Chi-tung county. Severe and moderate hepatitis were found only in indoor-caged injected animals with viremia and in some uninjected birds without viremia that had been kept in outdoor flocks. The latter hepatitis, as some hepatitis in adult Chinese ducks, may not be related to DHBV. Mild and insignificant hepatitis were also found in injected and noninjected ducklings, some of which had the vertically transmitted spontaneous viremia previously described. The good correlation of experimentally induced viremia with incidence and severity of hepatitis in the Pekin duckling provides a simple, rapid, and relatively inexpensive model to study the relation of lesions to hepatitis B family infection in nonprimates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ganem D., Weiser B., Barchuk A., Brown R. J., Varmus H. E. Biological characterization of acute infection with ground squirrel hepatitis virus. J Virol. 1982 Oct;44(1):366–373. doi: 10.1128/jvi.44.1.366-373.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Kam W., Rall L. B., Smuckler E. A., Schmid R., Rutter W. J. Hepatitis B viral DNA in liver and serum of asymptomatic carriers. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7522–7526. doi: 10.1073/pnas.79.23.7522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Marion P. L., Knight S. S., Feitelson M. A., Oshiro L. S., Robinson W. S. Major polypeptide of duck hepatitis B surface antigen particles. J Virol. 1983 Nov;48(2):534–541. doi: 10.1128/jvi.48.2.534-541.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Marion P. L., Knight S. S., Salazar F. H., Popper H., Robinson W. S. Ground squirrel hepatitis virus infection. Hepatology. 1983 Jul-Aug;3(4):519–527. doi: 10.1002/hep.1840030408. [DOI] [PubMed] [Google Scholar]
  5. Marion P. L., Oshiro L. S., Regnery D. C., Scullard G. H., Robinson W. S. A virus in Beechey ground squirrels that is related to hepatitis B virus of humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2941–2945. doi: 10.1073/pnas.77.5.2941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Marion P. L., Salazar F. H., Alexander J. J., Robinson W. S. State of hepatitis B viral DNA in a human hepatoma cell line. J Virol. 1980 Feb;33(2):795–806. doi: 10.1128/jvi.33.2.795-806.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mason W. S., Aldrich C., Summers J., Taylor J. M. Asymmetric replication of duck hepatitis B virus DNA in liver cells: Free minus-strand DNA. Proc Natl Acad Sci U S A. 1982 Jul;79(13):3997–4001. doi: 10.1073/pnas.79.13.3997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mason W. S., Seal G., Summers J. Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus. J Virol. 1980 Dec;36(3):829–836. doi: 10.1128/jvi.36.3.829-836.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'Connell A. P., Urban M. K., London W. T. Naturally occurring infection of Pekin duck embryos by duck hepatitis B virus. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1703–1706. doi: 10.1073/pnas.80.6.1703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Omata M., Uchiumi K., Ito Y., Yokosuka O., Mori J., Terao K., Wei-Fa Y., O'Connell A. P., London W. T., Okuda K. Duck hepatitis B virus and liver diseases. Gastroenterology. 1983 Aug;85(2):260–267. [PubMed] [Google Scholar]
  11. Patterson M., Gonzalez-Vitale J. C., Fagan C. J. Polycystic liver disease: a study of cyst fluid constituents. Hepatology. 1982 Jul-Aug;2(4):475–478. doi: 10.1002/hep.1840020414. [DOI] [PubMed] [Google Scholar]
  12. Popper H., Shih J. W., Gerin J. L., Wong D. C., Hoyer B. H., London W. T., Sly D. L., Purcell R. H. Woodchuck hepatitis and hepatocellular carcinoma: correlation of histologic with virologic observations. Hepatology. 1981 Mar-Apr;1(2):91–98. doi: 10.1002/hep.1840010202. [DOI] [PubMed] [Google Scholar]
  13. Shafritz D. A., Shouval D., Sherman H. I., Hadziyannis S. J., Kew M. C. Integration of hepatitis B virus DNA into the genome of liver cells in chronic liver disease and hepatocellular carcinoma. Studies in percutaneous liver biopsies and post-mortem tissue specimens. N Engl J Med. 1981 Oct 29;305(18):1067–1073. doi: 10.1056/NEJM198110293051807. [DOI] [PubMed] [Google Scholar]
  14. Summers J., Mason W. S. Replication of the genome of a hepatitis B--like virus by reverse transcription of an RNA intermediate. Cell. 1982 Jun;29(2):403–415. doi: 10.1016/0092-8674(82)90157-x. [DOI] [PubMed] [Google Scholar]
  15. Summers J., Smolec J. M., Snyder R. A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4533–4537. doi: 10.1073/pnas.75.9.4533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Summers J. Three recently described animal virus models for human hepatitis B virus. Hepatology. 1981 Mar-Apr;1(2):179–183. doi: 10.1002/hep.1840010215. [DOI] [PubMed] [Google Scholar]
  17. Tanaka K., Mori W., Suwa K. Victoria blue-nuclear fast red stain for HBs antigen detection in paraffin section. Acta Pathol Jpn. 1981 Jan;31(1):93–98. doi: 10.1111/j.1440-1827.1981.tb00987.x. [DOI] [PubMed] [Google Scholar]

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