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. 1999 Sep;31(1-2):29–35. doi: 10.1023/A:1008055702129

De novo adipogenesis for reconstructive surgery

Yasuo Kitagawa, Nobuko Kawaguchi
PMCID: PMC3449770  PMID: 19003121

Abstract

Autografting of lost soft tissue is an important subject of the plastic and reconstructive surgery and autograft of fat pads has been only technique for this goal. However, the results are disappointing because of absorption of the grafts with time. Adipoblasts or adipocyte precursor cells distribute widely in connective tissues and they can proliferate and mature into adipocytes even in the adult body. In experiments using mice, we found that de novo adipogenesis of endogenous precursor cells can be induced by injecting reconstituted basement membrane, Matrigel, supplemented with more than 1 ng/ml of bFGF. This adipogenesis was reproducibly induced by subcutaneous injection over the chest, lateral abdomen or head. Adipogenesis was induced even in ear cartilage or in muscle. To evaluate the possibility of future application of this de novo adipogenesis to plastic and reconstructive surgery, we have reviewed updated knowledge of the adipogenesis.

Keywords: adipogenesis, adipocyte determination and differentiation-dependent factor 1 (ADD1), basement membrane, basic fibroblast growth factor (bFGF), CCAAT-enhancer-binding proteins (C/EBPs), peroxisome proliferator activated receptors (PPARs), reconstructive surgery

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