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. 1984 Apr;81(8):2290–2294. doi: 10.1073/pnas.81.8.2290

High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.

E Jay, J Rommens, L Pomeroy-Cloney, D MacKnight, C Lutze-Wallace, P Wishart, D Harrison, W Y Lui, V Asundi, M Dawood, et al.
PMCID: PMC345044  PMID: 6326118

Abstract

A chemically synthesized gene for human interferon-gamma has been cloned into a prokaryotic expression vector under the regulation of a synthetic constitutive transcriptional-translational control unit that contains a strong bacteriophage T5 early promoter and a strong ribosome-binding site. Cells harboring the recombinant plasmid express high levels (4 X 10(9) units per liter of culture) of antiviral activity specific for interferon-gamma. Analysis of total cell lysates on NaDodSO4/polyacrylamide gels revealed a 17,200-dalton protein, expected for the nonglycosylated form of human interferon-gamma, that constitutes greater than 15% of total cell protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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