Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Apr;81(8):2504–2507. doi: 10.1073/pnas.81.8.2504

Segregation patterns of polymorphic restriction sites of the gene encoding the alpha subunit of human chorionic gonadotropin in trophoblastic disease.

M Hoshina, M R Boothby, R D Hussa, R A Pattillo, H M Camel, I Boime
PMCID: PMC345090  PMID: 6201859

Abstract

The gene encoding the alpha subunit of human chorionic gonadotropin contains at least two polymorphic sites in its 3' flanking region detected by restriction enzymes HindIII and EcoRI. We used these polymorphic sites as markers of tissue genotype in normal placenta, hydatidiform mole, choriocarcinoma, and peripheral leukocytes. As expected, inheritance patterns of most hydatidiform moles showed only a paternal genetic contribution. However, one uncommon DNA polymorphism pattern, homozygosity for the absence of the EcoRI site and the presence of the HindIII site, predominated in choriocarcinoma. Thus, our results suggest that moles which have this uncommon polymorphism pattern appear particularly likely to develop into choriocarcinoma.

Full text

PDF
2504

Images in this article

2505Image
on p.2505

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams J. M., Gerondakis S., Webb E., Mitchell J., Bernard O., Cory S. Transcriptionally active DNA region that rearranges frequently in murine lymphoid tumors. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6966–6970. doi: 10.1073/pnas.79.22.6966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boothby M., Ruddon R. W., Anderson C., McWilliams D., Boime I. A single gonadotropin alpha-subunit gene in normal tissue and tumor-derived cell lines. J Biol Chem. 1981 May 25;256(10):5121–5127. [PubMed] [Google Scholar]
  3. Clayton L. A., Tyrey L., Weed J. C., Jr, Hammond C. B. Endocrine aspects of trophoblastic neoplasia. J Reprod Med. 1981 Apr;26(4):192–199. [PubMed] [Google Scholar]
  4. Dawood M. Y., Saxena B. B., Landesman R. Human chorionic gonadotropin and its subunits in hydatidiform mole and choriocarcinoma. Obstet Gynecol. 1977 Aug;50(2):172–181. [PubMed] [Google Scholar]
  5. Fiddes J. C., Goodman H. M. The gene encoding the common alpha subunit of the four human glycoprotein hormones. J Mol Appl Genet. 1981;1(1):3–18. [PubMed] [Google Scholar]
  6. Fisher R. A., Sheppard D. M., Lawler S. D. Twin pregnancy with complete hydatidiform mole (46,XX) and fetus (46,XY): genetic origin proved by analysis of chromosome polymorphisms. Br Med J (Clin Res Ed) 1982 Apr 24;284(6324):1218–1220. doi: 10.1136/bmj.284.6324.1218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Forest J. C., Talbot J., Pagé M., Loiselle J. M. Tumour antigens associated with primary mediastinal choriocarcinoma. Can Med Assoc J. 1977 Dec 17;117(12):1386–1388. [PMC free article] [PubMed] [Google Scholar]
  8. Hayashi K., Bracken M. B., Freeman D. H., Jr, Hellenbrand K. Hydatidiform mole in the United States (1970-1977): a statistical and theoretical analysis. Am J Epidemiol. 1982 Jan;115(1):67–77. doi: 10.1093/oxfordjournals.aje.a113281. [DOI] [PubMed] [Google Scholar]
  9. Hussa R. O. Clinical utility of human chorionic gonadotropin and alpha-subunit measurements. Obstet Gynecol. 1982 Jul;60(1):1–12. [PubMed] [Google Scholar]
  10. Jacobs P. A., Wilson C. M., Sprenkle J. A., Rosenshein N. B., Migeon B. R. Mechanism of origin of complete hydatidiform moles. Nature. 1980 Aug 14;286(5774):714–716. doi: 10.1038/286714a0. [DOI] [PubMed] [Google Scholar]
  11. Kajii T., Ohama K. Androgenetic origin of hydatidiform mole. Nature. 1977 Aug 18;268(5621):633–634. doi: 10.1038/268633a0. [DOI] [PubMed] [Google Scholar]
  12. Kan Y. W., Dozy A. M., Trecartin R., Todd D. Identification of a nondeletion defect in alpha-thalassemia. N Engl J Med. 1977 Nov 17;297(20):1081–1084. doi: 10.1056/NEJM197711172972002. [DOI] [PubMed] [Google Scholar]
  13. Lawler S. D., Fisher R. A., Pickthall V. J., Povey S., Evans M. W. Genetic studies on hydatidiform moles. I. The origin of partial moles. Cancer Genet Cytogenet. 1982 Apr;5(4):309–320. doi: 10.1016/0165-4608(82)90096-6. [DOI] [PubMed] [Google Scholar]
  14. Lieblich J. M., Weintraub B. D., Rosen S. W., Chou J. Y., Robinson J. C. HeLa cells secrete alpha subunit of glycoprotein tropic hormones. Nature. 1976 Apr 8;260(5551):530–532. doi: 10.1038/260530a0. [DOI] [PubMed] [Google Scholar]
  15. Marcu K. B., Harris L. J., Stanton L. W., Erikson J., Watt R., Croce C. M. Transcriptionally active c-myc oncogene is contained within NIARD, a DNA sequence associated with chromosome translocations in B-cell neoplasia. Proc Natl Acad Sci U S A. 1983 Jan;80(2):519–523. doi: 10.1073/pnas.80.2.519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mochizuki M., Morikawa H., Kawaguchi K., Tojo S. Growth hormone, prolactin and chorionic somatomammotropin in normal and molar pregnancy. J Clin Endocrinol Metab. 1976 Sep;43(3):614–621. doi: 10.1210/jcem-43-3-614. [DOI] [PubMed] [Google Scholar]
  17. Mushinski J. F., Potter M., Bauer S. R., Reddy E. P. DNA rearrangement and altered RNA expression of the c-myb oncogene in mouse plasmacytoid lymphosarcomas. Science. 1983 May 20;220(4599):795–798. doi: 10.1126/science.6687762. [DOI] [PubMed] [Google Scholar]
  18. Ohama K., Kajii T., Okamoto E., Fukuda Y., Imaizumi K., Tsukahara M., Kobayashi K., Hagiwara K. Dispermic origin of XY hydatidiform moles. Nature. 1981 Aug 6;292(5823):551–552. doi: 10.1038/292551a0. [DOI] [PubMed] [Google Scholar]
  19. Seppälä M., Rutanen E. M., Ranta T., Aho I., Nieminen U., Unnérus H. A., Saksela E. Choriocarcinoma: expression of tumor- and trophoblast-associated antigens in patients with low chorionic gonadotropin excretion. Cancer. 1976 Nov;38(5):2065–2070. doi: 10.1002/1097-0142(197611)38:5<2065::aid-cncr2820380531>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  20. Shen-Ong G. L., Keath E. J., Piccoli S. P., Cole M. D. Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas. Cell. 1982 Dec;31(2 Pt 1):443–452. doi: 10.1016/0092-8674(82)90137-4. [DOI] [PubMed] [Google Scholar]
  21. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  22. Stanbridge E. J., Rosen S. W., Sussman H. H. Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6242–6245. doi: 10.1073/pnas.79.20.6242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Surti U., Szulman A. E., O'Brien S. Complete (classic) hydatidiform mole with 46,XY karyotype of paternal origin. Hum Genet. 1979 Oct 1;51(2):153–155. doi: 10.1007/BF00287169. [DOI] [PubMed] [Google Scholar]
  24. Taub R., Kirsch I., Morton C., Lenoir G., Swan D., Tronick S., Aaronson S., Leder P. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7837–7841. doi: 10.1073/pnas.79.24.7837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vaitukaitis J. L., Ross G. T., Braunstein G. D., Rayford P. L. Gonadotropins and their subunits: basic and clinical studies. Recent Prog Horm Res. 1976;32:289–331. doi: 10.1016/b978-0-12-571132-6.50019-1. [DOI] [PubMed] [Google Scholar]
  26. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES