Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 May;81(10):2965–2969. doi: 10.1073/pnas.81.10.2965

tau, a repeated DNA sequence in yeast.

G E Chisholm, F S Genbauffe, T G Cooper
PMCID: PMC345201  PMID: 6328481

Abstract

We have found a 371-base-pair (bp) repeated DNA element, tau, in Saccharomyces cerevisiae. The ends of tau are composed of a 5-bp inverted repeat, similar in sequence to those reported for the Ty, sigma, copia, and spleen necrosis virus elements. These inverted repeats are flanked by 5-bp direct repeats of a target sequence that occurs only once in an allele that lacks the tau element. This overall structure is characteristic of transposable elements. Like sigma, tau elements have been found (in both orientations) closely associated with tRNA genes (409 and 198 bp from the 5' end, respectively). It is noteworthy that one representative of tau was isolated in a concentric insertion of tau, delta, and sigma.

Full text

PDF
2969

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brodeur G. M., Sandmeyer S. B., Olson M. V. Consistent association between sigma elements and tRNA genes in yeast. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3292–3296. doi: 10.1073/pnas.80.11.3292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Calos M. P., Miller J. H. Transposable elements. Cell. 1980 Jul;20(3):579–595. doi: 10.1016/0092-8674(80)90305-0. [DOI] [PubMed] [Google Scholar]
  3. Cameron J. R., Davis R. W. The effects of Escherichia coli and yeast DNA insertions on the growth of lambda bacteriophage. Science. 1977 Apr 8;196(4286):212–215. doi: 10.1126/science.322285. [DOI] [PubMed] [Google Scholar]
  4. Cameron J. R., Loh E. Y., Davis R. W. Evidence for transposition of dispersed repetitive DNA families in yeast. Cell. 1979 Apr;16(4):739–751. doi: 10.1016/0092-8674(79)90090-4. [DOI] [PubMed] [Google Scholar]
  5. Cummins C. M., Donahue T. F., Culbertson M. R. Nucleotide sequence of the SUF2 frameshift suppressor gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3565–3569. doi: 10.1073/pnas.79.11.3565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duncan C. H., Jagadeeswaran P., Wang R. R., Weissman S. M. Structural analysis of templates and RNA polymerase III transcripts of Alu family sequences interspersed among the human beta-like globin genes. Gene. 1981 Mar;13(2):185–196. doi: 10.1016/0378-1119(81)90007-x. [DOI] [PubMed] [Google Scholar]
  7. Dunsmuir P., Brorein W. J., Jr, Simon M. A., Rubin G. M. Insertion of the Drosophila transposable element copia generates a 5 base pair duplication. Cell. 1980 Sep;21(2):575–579. doi: 10.1016/0092-8674(80)90495-x. [DOI] [PubMed] [Google Scholar]
  8. Eigel A., Olah J., Feldmann H. Structural comparison of two yeast tRNA Glu 3 genes. Nucleic Acids Res. 1981 Jun 25;9(12):2961–2970. doi: 10.1093/nar/9.12.2961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Erdmann V. A. Collection of published 5S and 5.8S RNA sequences and their precursors. Nucleic Acids Res. 1981 Jan 10;9(1):r25–r42. doi: 10.1093/nar/9.1.213-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Errede B., Cardillo T. S., Sherman F., Dubois E., Deschamps J., Wiame J. M. Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes. Cell. 1980 Nov;22(2 Pt 2):427–436. doi: 10.1016/0092-8674(80)90353-0. [DOI] [PubMed] [Google Scholar]
  11. Farabaugh P. J., Fink G. R. Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication. Nature. 1980 Jul 24;286(5771):352–356. doi: 10.1038/286352a0. [DOI] [PubMed] [Google Scholar]
  12. Feldmann H., Olah J., Friedenreich H. Sequence of a yeast DNA fragment containing a chromosomal replicator and a tRNA Glu 3 gene. Nucleic Acids Res. 1981 Jun 25;9(12):2949–2959. doi: 10.1093/nar/9.12.2949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gafner J., Philippsen P. The yeast transposon Ty1 generates duplications of target DNA on insertion. Nature. 1980 Jul 24;286(5771):414–418. doi: 10.1038/286414a0. [DOI] [PubMed] [Google Scholar]
  14. Gafner J., Robertis E. M., Philippsen P. Delta sequences in the 5' non-coding region of yeast tRNA genes. EMBO J. 1983;2(4):583–591. doi: 10.1002/j.1460-2075.1983.tb01467.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Goodman H. M., Olson M. V., Hall B. D. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453–5457. doi: 10.1073/pnas.74.12.5453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hosbach H. A., Silberklang M., McCarthy B. J. Evolution of a D. melanogaster glutamate tRNA gene cluster. Cell. 1980 Aug;21(1):169–178. doi: 10.1016/0092-8674(80)90124-5. [DOI] [PubMed] [Google Scholar]
  18. Hovemann B., Sharp S., Yamada H., Söll D. Analysis of a drosophila tRNA gene cluster. Cell. 1980 Apr;19(4):889–895. doi: 10.1016/0092-8674(80)90080-x. [DOI] [PubMed] [Google Scholar]
  19. Kleckner N. Transposable elements in prokaryotes. Annu Rev Genet. 1981;15:341–404. doi: 10.1146/annurev.ge.15.120181.002013. [DOI] [PubMed] [Google Scholar]
  20. Levis R., Dunsmuir P., Rubin G. M. Terminal repeats of the Drosophila transposable element copia: nucleotide sequence and genomic organization. Cell. 1980 Sep;21(2):581–588. doi: 10.1016/0092-8674(80)90496-1. [DOI] [PubMed] [Google Scholar]
  21. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Müller F., Clarkson S. G. Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA. Cell. 1980 Feb;19(2):345–353. doi: 10.1016/0092-8674(80)90509-7. [DOI] [PubMed] [Google Scholar]
  23. Olah J., Feldmann H. Structure of a yeast non-initiating methionine-tRNA gene. Nucleic Acids Res. 1980 May 10;8(9):1975–1986. doi: 10.1093/nar/8.9.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Olson M. V., Page G. S., Sentenac A., Piper P. W., Worthington M., Weiss R. B., Hall B. D. Only one of two closely related yeast suppressor tRNA genes contains an intervening sequence. Nature. 1981 Jun 11;291(5815):464–469. doi: 10.1038/291464a0. [DOI] [PubMed] [Google Scholar]
  25. Reddy R., Ro-Choi T. S., Henning D., Busch H. Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells. J Biol Chem. 1974 Oct 25;249(20):6486–6494. [PubMed] [Google Scholar]
  26. Roeder G. S., Farabaugh P. J., Chaleff D. T., Fink G. R. The origins of gene instability in yeast. Science. 1980 Sep 19;209(4463):1375–1380. doi: 10.1126/science.6251544. [DOI] [PubMed] [Google Scholar]
  27. Sandmeyer S. B., Olson M. V. Insertion of a repetitive element at the same position in the 5'-flanking regions of two dissimilar yeast tRNA genes. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7674–7678. doi: 10.1073/pnas.79.24.7674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schmidt O., Mao J., Ogden R., Beckmann J., Sakano H., Abelson J., Söll D. Dimeric tRNA precursors in yeast. Nature. 1980 Oct 23;287(5784):750–752. doi: 10.1038/287750a0. [DOI] [PubMed] [Google Scholar]
  29. Shimotohno K., Mizutani S., Temin H. M. Sequence of retrovirus provirus resembles that of bacterial transposable elements. Nature. 1980 Jun 19;285(5766):550–554. doi: 10.1038/285550a0. [DOI] [PubMed] [Google Scholar]
  30. Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Standring D. N., Venegas A., Rutter W. J. Yeast tRNA3Leu gene transcribed and spliced in a HeLa cell extract. Proc Natl Acad Sci U S A. 1981 Oct;78(10):5963–5967. doi: 10.1073/pnas.78.10.5963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Sumrada R. A., Cooper T. G. Isolation of the CAR1 gene from Saccharomyces cerevisiae and analysis of its expression. Mol Cell Biol. 1982 Dec;2(12):1514–1523. doi: 10.1128/mcb.2.12.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Valenzuela P., Venegas A., Weinberg F., Bishop R., Rutter W. J. Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA. Proc Natl Acad Sci U S A. 1978 Jan;75(1):190–194. doi: 10.1073/pnas.75.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Williamson V. M. Transposable elements in yeast. Int Rev Cytol. 1983;83:1–25. doi: 10.1016/s0074-7696(08)61684-8. [DOI] [PubMed] [Google Scholar]
  35. Williamson V. M., Young E. T., Ciriacy M. Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II. Cell. 1981 Feb;23(2):605–614. doi: 10.1016/0092-8674(81)90156-2. [DOI] [PubMed] [Google Scholar]
  36. del Rey F. J., Donahue T. F., Fink G. R. sigma, a repetitive element found adjacent to tRNA genes of yeast. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4138–4142. doi: 10.1073/pnas.79.13.4138. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES