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. 1984 Jul;81(13):4188–4192. doi: 10.1073/pnas.81.13.4188

Properties of retrovirus-like particles produced by a human breast carcinoma cell line: immunological relationship with mouse mammary tumor virus proteins.

I Keydar, T Ohno, R Nayak, R Sweet, F Simoni, F Weiss, S Karby, R Mesa-Tejada, S Spiegelman
PMCID: PMC345394  PMID: 6330748

Abstract

Clonal derivatives 8 and 11 of the T47D human breast carcinoma cell line release particles that have the biochemical characteristics of a retrovirus. Particles recovered from cultures of [3H]uridine-labeled clone 11 had a density of 1.18 g/ml and contained 60-70S and 35S RNAs associated with reverse transcriptase activity. The production of these particles was steroid-dependent. Clone 8 particles had a higher density, 1.195 g/ml, and their production was independent of steroid hormone. By RIA, antigens crossreactive with the 52,000-dalton envelope glycoprotein gp52, the major external protein of mouse mammary tumor virus, were found associated with these particles and in the media. Most of the gp52-related antigen was in soluble form, but it was enriched in the particle preparation. A lesser amount of antigen was distributed within the cultured cells. Absorption of rabbit antibody to gp52 with clone 11 particle preparations eliminated the ability of this antibody to detect immunocytochemically a crossreactive antigen previously localized in tissue sections of human breast carcinoma. These results indicate that the particle isolates from T47D contain the same gp52-related antigen found in human breast carcinomas and constitute an excellent source for the purification and characterization of this antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axel R., Gulati S. C., Spiegelman S. Particles containing RNA-instructed DNA polymerase and virus-related RNA in human breast cancers. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3133–3137. doi: 10.1073/pnas.69.11.3133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bausch J. N., Prasad K. S., Spiegelman S. Radioimmunolocalization of murine mammary tumor virus proteins in gels. Anal Biochem. 1982 Jan 15;119(2):293–298. doi: 10.1016/0003-2697(82)90588-7. [DOI] [PubMed] [Google Scholar]
  3. Callahan R., Drohan W., Tronick S., Schlom J. Detection and cloning of human DNA sequences related to the mouse mammary tumor virus genome. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5503–5507. doi: 10.1073/pnas.79.18.5503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Holder W. D., Jr, Wells S. A., Jr Antibody reacting with the murine mammary tumor virus in the serum of patients with breast carcinoma: a possible serological detection method for breast carcinoma. Cancer Res. 1983 Jan;43(1):239–244. [PubMed] [Google Scholar]
  5. Keydar I., Chen L., Karby S., Weiss F. R., Delarea J., Radu M., Chaitcik S., Brenner H. J. Establishment and characterization of a cell line of human breast carcinoma origin. Eur J Cancer. 1979 May;15(5):659–670. doi: 10.1016/0014-2964(79)90139-7. [DOI] [PubMed] [Google Scholar]
  6. Lloyd R. V., Rosen P. P., Sarkar N. H., Jimenez D., Kinne D. W., Menendez-Botet C., Schwartz M. K. Murine mammary tumor virus related antigen in human male mammary carcinoma. Cancer. 1983 Feb 15;51(4):654–661. doi: 10.1002/1097-0142(19830215)51:4<654::aid-cncr2820510418>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  7. Mesa-Tejada R., Keydar I., Ramanarayanan M., Ohno T., Fenoglio C., Spiegelman S. Detection in human breast carcinomas of an antigen immunologically related to a group-specific antigen of mouse mammary tumor virus. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1529–1533. doi: 10.1073/pnas.75.3.1529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mesa-Tejada R., Oster M. W., Fenoglio C. M., Magidson J., Spiegelman S. Diagnosis of primary breast carcinoma through immunohistochemical detection of antigen related to mouse mammary tumor virus in metastatic lesions: a report of two cases. Cancer. 1982 Jan 15;49(2):261–268. doi: 10.1002/1097-0142(19820115)49:2<261::aid-cncr2820490211>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  9. Michalides R., Spiegelman S., Schlom J. Biochemical characterization of putative subviral particulates from human malignant breast tumors. Cancer Res. 1975 Apr;35(4):1003–1008. [PubMed] [Google Scholar]
  10. Ohno T., Mesa-Tejada R., Keydar I., Ramanarayanan M., Bausch J., Spiegelman S. Human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus. Proc Natl Acad Sci U S A. 1979 May;76(5):2460–2464. doi: 10.1073/pnas.76.5.2460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ohno T., Sweet R. W., Dejak D., Spiegelman S. Purification and characterization of the DNA polymerase of human breast cancer particles. Proc Natl Acad Sci U S A. 1977 Feb;74(2):764–768. doi: 10.1073/pnas.74.2.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Poon M. C., Tomana M., Niedermeier W. Serum antibodies against mouse mammary tumor-virus-associated antigen detected nine months before appearance of a breast carcinoma. Ann Intern Med. 1983 Jun;98(6):937–938. doi: 10.7326/0003-4819-98-6-937. [DOI] [PubMed] [Google Scholar]
  13. Ritzi E., Martin D. S., Stolfi R. L., Spiegelman S. Plasma levels of a viral protein as a diagnostic signal for the presence of mammary tumor: the effect of tumor removal. J Exp Med. 1977 Apr 1;145(4):999–1013. doi: 10.1084/jem.145.4.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ritzi E., Martin D. S., Stolfi R. L., Spiegelman S. Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4190–4194. doi: 10.1073/pnas.73.11.4190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vaidya A. B., Black M. M., Dion A. S., Moore D. H. Homology between human breast tumour RNA and mouse mammary tumour virus genome. Nature. 1974 Jun 7;249(457):565–567. doi: 10.1038/249565a0. [DOI] [PubMed] [Google Scholar]

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