Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jun;81(11):3263–3267. doi: 10.1073/pnas.81.11.3263

DNA and RNA virus species are inhibited by xanthates, a class of antiviral compounds with unique properties.

G Sauer, E Amtmann, K Melber, A Knapp, K Müller, K Hummel, A Scherm
PMCID: PMC345487  PMID: 6328507

Abstract

Various DNA and RNA virus species are inhibited by xanthate compounds at concentrations that leave the mitotic activity of uninfected cells unimpaired. The concentration of tricyclodecan -9-yl- xanthogenate that reduces the yield of herpes simplex virus types 1 and 2 by 50% is between 4.5 and 33 microM. The replication of DNA viruses such as simian virus 40 can be blocked at the DNA and RNA level both early and late after infection. The xanthates are not incorporated into nucleic acids. Episomal bovine papilloma virus DNA replication and transcription are also inhibited in transformed cells. The treated cells revert to the normal phenotype by acquisition of contact inhibition and a flat morphology.

Full text

PDF
3266

Images in this article

3266Image
on p.3266
3266Image
on p.3266

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amtmann E., Sauer G. Bovine papilloma virus transcription: polyadenylated RNA species and assessment of the direction of transcription. J Virol. 1982 Jul;43(1):59–66. doi: 10.1128/jvi.43.1.59-66.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
  3. Collins P., Appleyard G., Oliver N. M. Sensitivity of herpes virus isolates from acyclovir clinical trials. Am J Med. 1982 Jul 20;73(1A):380–382. doi: 10.1016/0002-9343(82)90127-9. [DOI] [PubMed] [Google Scholar]
  4. Elion G. B., Furman P. A., Fyfe J. A., de Miranda P., Beauchamp L., Schaeffer H. J. Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5716–5720. doi: 10.1073/pnas.74.12.5716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Field H. J., Neden J. Isolation of bromovinyldeoxyuridine-resistant strains of herpes simplex virus and successful chemotherapy of mice infected with one such strain by using acyclovir. Antiviral Res. 1982 Oct;2(5):243–254. doi: 10.1016/0166-3542(82)90048-1. [DOI] [PubMed] [Google Scholar]
  6. Galasso G. J. An assessment of antiviral drugs for the management of infectious diseases in humans. Antiviral Res. 1981 Jun;1(2):73–96. doi: 10.1016/0166-3542(81)90035-8. [DOI] [PubMed] [Google Scholar]
  7. Helgstrand E., Eriksson B., Johansson N. G., Lannerö B., Larsson A., Misiorny A., Norén J. O., Sjöberg B., Stenberg K., Stening G. Trisodium phosphonoformate, a new antiviral compound. Science. 1978 Sep 1;201(4358):819–821. doi: 10.1126/science.210500. [DOI] [PubMed] [Google Scholar]
  8. Krieg P., Amtmann E., Sauer G., Lavi S., Kleinberger T., Winocour E. The integrated SV40 genome in permissive transformed monkey cells. Virology. 1981 Jan 30;108(2):453–461. doi: 10.1016/0042-6822(81)90452-9. [DOI] [PubMed] [Google Scholar]
  9. Krieg P., Amtmann E., Sauer G. The simultaneous extraction of high-molecular-weight DNA and of RNA from solid tumors. Anal Biochem. 1983 Oct 15;134(2):288–294. doi: 10.1016/0003-2697(83)90299-3. [DOI] [PubMed] [Google Scholar]
  10. Law M. F., Lowy D. R., Dvoretzky I., Howley P. M. Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proc Natl Acad Sci U S A. 1981 May;78(5):2727–2731. doi: 10.1073/pnas.78.5.2727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MUNK K., DONNER D. CYTOPATHISCHER EFFEKT UND PLAQUE-MORPHOLOGIE VERSCHIEDENER HERPES-SIMPLEX-VIRUSSTAEMME. Arch Gesamte Virusforsch. 1963 Aug 26;13:529–540. [PubMed] [Google Scholar]
  12. Marsh M., Matlin K., Simons K., Reggio H., White J., Kartenbeck J., Helenius A. Are lysosomes a site of enveloped-virus penetration? Cold Spring Harb Symp Quant Biol. 1982;46(Pt 2):835–843. doi: 10.1101/sqb.1982.046.01.078. [DOI] [PubMed] [Google Scholar]
  13. Marsh M., Matlin K., Simons K., Reggio H., White J., Kartenbeck J., Helenius A. Are lysosomes a site of enveloped-virus penetration? Cold Spring Harb Symp Quant Biol. 1982;46(Pt 2):835–843. doi: 10.1101/sqb.1982.046.01.078. [DOI] [PubMed] [Google Scholar]
  14. May G., Peteri D., Hummel K. Uber Synthese und virustatische Wirkung von Adamantan-1-carbonsäureamidinen. Arzneimittelforschung. 1978;28(5):732–735. [PubMed] [Google Scholar]
  15. Munk K., Ludwig G. Properties of plaque variants of herpes virus hominis strains of genital origin. Arch Gesamte Virusforsch. 1972;37(4):308–315. doi: 10.1007/BF01241453. [DOI] [PubMed] [Google Scholar]
  16. Pauls F. P., Dowdle W. R. A serologic study of herpesvirus hominis strains by microneutralization tests. J Immunol. 1967 May;98(5):941–947. [PubMed] [Google Scholar]
  17. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  18. Rösl F., Waldeck W., Sauer G. Isolation of episomal bovine papillomavirus chromatin and identification of a DNase I-hypersensitive region. J Virol. 1983 May;46(2):567–574. doi: 10.1128/jvi.46.2.567-574.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sauer G., Orth H. D., Munk K. Interference of actinomycin D with the replication of the herpes virus DNA. I. Difference in behaviour of cellular and viral nucleic acid synthesis following treatment with actinomycin D. Biochim Biophys Acta. 1966 May 19;119(2):331–340. doi: 10.1016/0005-2787(66)90191-2. [DOI] [PubMed] [Google Scholar]
  20. Schaeffer H. J., Beauchamp L., de Miranda P., Elion G. B., Bauer D. J., Collins P. 9-(2-hydroxyethoxymethyl) guanine activity against viruses of the herpes group. Nature. 1978 Apr 13;272(5654):583–585. doi: 10.1038/272583a0. [DOI] [PubMed] [Google Scholar]
  21. Schlegel R., Dickson R. B., Willingham M. C., Pastan I. H. Amantadine and dansylcadaverine inhibit vesicular stomatitis virus uptake and receptor-mediated endocytosis of alpha 2-macroglobulin. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2291–2295. doi: 10.1073/pnas.79.7.2291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shah K. V., Ozer H. L., Ghazey H. N., Kelly T. J., Jr Common structural antigen of papovaviruses of the simian virus 40-polyoma subgroup. J Virol. 1977 Jan;21(1):179–186. doi: 10.1128/jvi.21.1.179-186.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  24. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Timbury M. C. Temperature-sensitive mutants of herpes simplex virus type 2. J Gen Virol. 1971 Nov;13(2):373–376. doi: 10.1099/0022-1317-13-2-373. [DOI] [PubMed] [Google Scholar]
  26. Van Leuven F., Cassiman J. J., Van Den Berghe H. Primary amines inhibit recycling of alpha 2M receptors in fibroblasts. Cell. 1980 May;20(1):37–43. doi: 10.1016/0092-8674(80)90232-9. [DOI] [PubMed] [Google Scholar]
  27. Yakobson E., Prives C., Hartman J. R., Winocour E., Revel M. Inhibition of viral protein synthesis in monkey cells treated with interferon late in simian virus 40 lytic cycle. Cell. 1977 Sep;12(1):73–81. doi: 10.1016/0092-8674(77)90186-6. [DOI] [PubMed] [Google Scholar]
  28. de Duve C., de Barsy T., Poole B., Trouet A., Tulkens P., Van Hoof F. Commentary. Lysosomotropic agents. Biochem Pharmacol. 1974 Sep 15;23(18):2495–2531. doi: 10.1016/0006-2952(74)90174-9. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES