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. 1982 Jan;79(1):46–50. doi: 10.1073/pnas.79.1.46

Transposon-specified site-specific recombination.

P Kitts, L Symington, M Burke, R Reed, D Sherratt
PMCID: PMC345658  PMID: 6275390

Abstract

Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product interchangeable with the Tn1/3tnpR protein in promoting Tn1/3 site-specific recombination. These data support the hypothesis that cointegrates containing directly repeated copies of Tn1/3 are obligatory intermediates in interreplicon transposition of Tn1/3. In addition, we show here that the reaction is independent of the element-encoded tnpA gene product. Tn501, which specifies mercury resistance, also produces cointegrates as intermediates in interreplicon transposition. The appearance of Tn501-specified recombination activity that can act on these cointegrates requires growth of cells in the presence of Hg2+.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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