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. 1982 Jan;79(1):123–126. doi: 10.1073/pnas.79.1.123

Mitochondria are associated with microtubules and not with intermediate filaments in cultured fibroblasts.

E H Ball, S J Singer
PMCID: PMC345674  PMID: 6275382

Abstract

Triple-immunofluorescence experiments with antibodies to cytochrome c oxidase, tubulin, and vimentin have been used to immunolabel the mitochondria, microtubules, and intermediate filaments inside the same cultured fibroblasts. In particular, fibroblasts were immunolabeled after they had either been transformed by infection with Rous sarcoma virus or given long-term treatment with cycloheximide. These treatments induced redistribution of the intermediate filaments into a perinuclear arrangement, segregated away from the microtubules, which remained extended to the cell periphery. In such cells, many labeled mitochondria were observed to be codistributed with the peripherally located microtubules. From these results, we infer that an association, probably involving some type of chemical linkage(s), between mitochondria and microtubules exists in these cells that is independent of the intermediate filaments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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