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. 1982 May;79(10):3340–3344. doi: 10.1073/pnas.79.10.3340

Interaction of the Escherichia coli dnaA initiation protein with the dnaZ polymerization protein in vivo.

J R Walker, J A Ramsey, W G Haldenwang
PMCID: PMC346411  PMID: 6285347

Abstract

To define in vivo interactions of Escherichia coli DNA replication components, extragenic suppressors of a dnaZ(TS) mutant were isolated. A temperature-sensitive dnaZ mutant, which is defective in polymerization, was placed at 39 degrees C to select temperature-insensitive revertants. Some of these revertants also were cold sensitive, a phenotypic property that facilitated study of the suppressor. Mapping of the cold sensitivity indicated that some of the suppressor mutations are intragenic but others are located within the initiation gene, dnaA. The dnaA mutations that suppress the dnaZ(TS) defect are designated dnaA(SUZ, CS). The dnaA(SUZ, CS) strains have a defect in DNA synthesis at low temperature that is typical of an initiation defect. These data suggest that the dnaA product, an initiation factor, interacts in vivo with the dnaZ protein, a polymerization factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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