Abstract
The yellow chromophore of the methyl-coenzyme M methylreductase of Methanobacterium thermoautotrophicum has been found to be the nickel-containing factor F430. Treatment of 63Ni-labeled methylreductase with 80% aqueous methanol released the radiolabel as well as the yellow chromophore; both properties were associated with a single compound that was found to be identical to F430, the stoichiometry being 1 mol of nickel per mol of F430 and 2 mol of F430 per mol of methylreductase.
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