Abstract
Selected species of the order Peronosporales, which are unable to epoxidize squalene and thus synthesize sterols, are able to metabolize exogenous cycloartenol to lanosterol and in some organisms to fucosterol, ergosterol, and cholesterol. Lanosterol was less effectively utilized but some ergosterol and cholesterol were yielded. Fucosterol was very efficiently metabolized by most species to ergosterol, Δ7-ergostenol, Δ5-ergostenol, cholestanol, and cholesterol. Several unknown sterols were observed in most trials. These data suggest a vestigial sterol synthetic pathway derived from cycloartenol, followed by possible isomerization to lanosterol and then to other sterols.
Keywords: Pythiaceae, Phytophthora, Pythium, Peronophythora
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Elliott C. G., Knights B. A. Uptake and metabolism of cholesterol and cholesteryl oleate by Phytophthora cactorum. Biochim Biophys Acta. 1974 Jul 26;360(1):78–87. doi: 10.1016/0005-2760(74)90181-7. [DOI] [PubMed] [Google Scholar]
- Elliott C. G. Sterols in fungi: their functions in growth and reproduction. Adv Microb Physiol. 1977;15:121–173. doi: 10.1016/s0065-2911(08)60316-1. [DOI] [PubMed] [Google Scholar]
- Gibbons G. F., Goad L. J., Goodwin T. W., Nes W. R. Concerning the role of lanosterol and cycloartenol in steroid biosynthesis. J Biol Chem. 1971 Jun 25;246(12):3967–3976. [PubMed] [Google Scholar]
- McMorris T. C., White R. H. Cholesterol beta-D-glucoside-6'-O-palmitate, a metabolite of Pythium sylvaticum. Biochim Biophys Acta. 1977 Feb 23;486(2):308–312. doi: 10.1016/0005-2760(77)90026-1. [DOI] [PubMed] [Google Scholar]
- Svoboda J. A., Robbins W. E. Desmosterol as a common intermediate in the conversion of a number of C28 and C29 plant sterols to cholesterol by the tobacco hornworm. Experientia. 1968 Nov 15;24(11):1131–1132. doi: 10.1007/BF02147805. [DOI] [PubMed] [Google Scholar]
- Wood S. G., Gottlieb D. Evidence from cell-free systems for differences in the sterol biosynthetic pathway of Rhizoctonia solani and Phytophthora cinnamomi. Biochem J. 1978 Feb 15;170(2):355–363. doi: 10.1042/bj1700355. [DOI] [PMC free article] [PubMed] [Google Scholar]