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. 1982 Jun;79(12):3818–3822. doi: 10.1073/pnas.79.12.3818

Antibodies to RNA from autoimmune NZB/NZW mice recognize a similar antigenic determinant and show a large idiotypic diversity.

D Eilat, M Hochberg, R Fischel, R Laskov
PMCID: PMC346519  PMID: 6179087

Abstract

We have identified two RNA-specific hybridoma autoantibodies in fusions of spleen cells from unimmunized NZB/NZW female mice with BALB/c myeloma cells. The two fusion experiments were carried out 2 years apart with different myeloma partners. Specificity analyses showed that the two monoclonal antibodies and the total RNA-binding IgG in NZB/NZW serum recognize a G,C-rich sequence of ribonucleotides. The isolated heavy and light chains of the two antibodies and their papain Fab fragments could be distinguished by NaDodSO4/polyacrylamide gel electrophoresis. Rabbit anti-idiotypic antibodies prepared against the two monoclonal proteins showed unique specificities for the antigen-binding sites of their cognate auto-antibodies. Moreover, the anti-idiotypic antisera had little effect on the RNA-binding capacity of the total IgG from NZB/NZW serum. These results suggest that a wide range of different idiotypes is involved in the autoimmune response to a similar antigenic determinant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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