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. 1982 Sep;79(18):5494–5498. doi: 10.1073/pnas.79.18.5494

Reconstruction of the chloroplast noncyclic electron transport pathway from water to NADP with three integral protein complexes

Eric Lam *, Richard Malkin †,‡
PMCID: PMC346930  PMID: 16593225

Abstract

Reconstruction of photosynthetic noncyclic electron transport from water to NADP has been accomplished by using three integral protein complexes isolated from chloroplast thylakoid membranes: photosystems I and II and the cytochrome b6-f complex. This system shows an absolute dependence on the presence of all three protein complexes for NADP reduction, in addition to plastocyanin, ferredoxin, and ferredoxin-NADP reductase. The reconstructed system was found to be sensitive to low concentrations of known inhibitors of noncyclic electron transport. Depletion of the Rieske iron-sulfur center and bound plastoquinone from the cytochrome b6-f complex resulted in an inhibition of the photoreduction of NADP.

Keywords: photosystem I, photosystem II, cytochrome b6-f

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson B., Anderson J. M. Lateral heterogeneity in the distribution of chlorophyll-protein complexes of the thylakoid membranes of spinach chloroplasts. Biochim Biophys Acta. 1980 Dec 3;593(2):427–440. doi: 10.1016/0005-2728(80)90078-x. [DOI] [PubMed] [Google Scholar]
  2. Arnon D. I., Tsujimoto H. Y., Tang G. M. Proton transport in photooxidation of water: A new perspective on photosynthesis. Proc Natl Acad Sci U S A. 1981 May;78(5):2942–2946. doi: 10.1073/pnas.78.5.2942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arntzen C. J., Dilley R. A., Peters G. A., Shaw E. R. Photochemical activity and structural studies of photosystems derived from chloroplast grana and stroma lamellae. Biochim Biophys Acta. 1972 Jan 21;256(1):85–107. doi: 10.1016/0005-2728(72)90165-x. [DOI] [PubMed] [Google Scholar]
  4. Ben-Hayyim G., Gromet-Elhanan Z., Avron M. A specific and sensitive method for the determination of NADPH. Anal Biochem. 1969 Apr 4;28(1):6–12. doi: 10.1016/0003-2697(69)90151-1. [DOI] [PubMed] [Google Scholar]
  5. Cox R. P., Andersson B. Lateral and transverse organisation of cytochromes in the chloroplast thylakoid membrane. Biochem Biophys Res Commun. 1981 Dec 31;103(4):1336–1342. doi: 10.1016/0006-291x(81)90269-2. [DOI] [PubMed] [Google Scholar]
  6. HATEFI Y., HAAVIK A. G., FOWLER L. R., GRIFFITHS D. E. Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem. 1962 Aug;237:2661–2669. [PubMed] [Google Scholar]
  7. Haehnel W., Pröpper A., Krause H. Evidence for complexed plastocyanin as the immediate electron donor of P-700. Biochim Biophys Acta. 1980 Dec 3;593(2):384–399. doi: 10.1016/0005-2728(80)90075-4. [DOI] [PubMed] [Google Scholar]
  8. Hurt E., Hauska G. A cytochrome f/b6 complex of five polypeptides with plastoquinol-plastocyanin-oxidoreductase activity from spinach chloroplasts. Eur J Biochem. 1981 Jul;117(3):591–595. doi: 10.1111/j.1432-1033.1981.tb06379.x. [DOI] [PubMed] [Google Scholar]
  9. Hurt E., Hauska G., Malkin R. Isolation of the Rieske iron-sulfur protein from the cytochrome b6/f complex of spinach chloroplasts. FEBS Lett. 1981 Nov 2;134(1):1–5. doi: 10.1016/0014-5793(81)80537-6. [DOI] [PubMed] [Google Scholar]
  10. Ke B., Shaw E. R. Reconstitution of photosystems I and II using spinach subchloroplast fragments fractionated by triton treatment. Biochim Biophys Acta. 1972 Aug 17;275(2):192–198. doi: 10.1016/0005-2728(72)90040-0. [DOI] [PubMed] [Google Scholar]
  11. Malkin R., Bearden A. J. Membrane-bound iron-sulfur centers in photosynthetic systems. Biochim Biophys Acta. 1978 Oct 23;505(2):147–181. doi: 10.1016/0304-4173(78)90011-3. [DOI] [PubMed] [Google Scholar]
  12. Malkin R. Interaction of photosynthetic electron transport inhibitors and the Rieske Iron-Sulfur center in chloroplasts and the cytochrome b6-f complex. Biochemistry. 1982 Jun 8;21(12):2945–2950. doi: 10.1021/bi00541a022. [DOI] [PubMed] [Google Scholar]
  13. McSwain B. D., Arnon D. I. Enhancement effects and the identity of the two photochemical reactions of photosynthesis. Proc Natl Acad Sci U S A. 1968 Nov;61(3):989–996. doi: 10.1073/pnas.61.3.989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Melis A., Brown J. S. Stoichiometry of system I and system II reaction centers and of plastoquinone in different photosynthetic membranes. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4712–4716. doi: 10.1073/pnas.77.8.4712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mullet J. E., Burke J. J., Arntzen C. J. Chlorophyll proteins of photosystem I. Plant Physiol. 1980 May;65(5):814–822. doi: 10.1104/pp.65.5.814. [DOI] [PMC free article] [PubMed] [Google Scholar]

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