Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Sep;79(18):5631–5635. doi: 10.1073/pnas.79.18.5631

Restriction map variation in the Adh region of Drosophila.

C H Langley, E Montgomery, W F Quattlebaum
PMCID: PMC346958  PMID: 6291056

Abstract

Restriction maps of a 12,000-nucleotide region containing the alcohol dehydrogenase (Adh) locus of the second chromosome of Drosophila were determined for (i) 18 independent second chromosome stocks of D. melanogaster established from several natural populations and (ii) five closely related Drosophila species. All of the detected map differences appear to lie outside the Adh coding block. Four polymorphic restriction sites and four insertion/deletion variants were identified among D. melanogaster chromosomes. The estimated heterozygosity per nucleotide site was 0.006. The relative distributions of restriction sites and insertion/deletion variations among subpopulations and species suggest that the insertion/deletion variation may be mildly deleterious.

Full text

PDF
5631

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avise J. C., Lansman R. A., Shade R. O. The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations. I. Population structure and evolution in the genus Peromyscus. Genetics. 1979 May;92(1):279–295. doi: 10.1093/genetics/92.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barrie P. A., Jeffreys A. J., Scott A. F. Evolution of the beta-globin gene cluster in man and the primates. J Mol Biol. 1981 Jul 5;149(3):319–336. doi: 10.1016/0022-2836(81)90476-9. [DOI] [PubMed] [Google Scholar]
  3. Bell G. I., Karam J. H., Rutter W. J. Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5759–5763. doi: 10.1073/pnas.78.9.5759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benyajati C., Place A. R., Powers D. A., Sofer W. Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein. Proc Natl Acad Sci U S A. 1981 May;78(5):2717–2721. doi: 10.1073/pnas.78.5.2717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bernards R., Flavell R. A. Physical mapping of the globin gene deletion in hereditary persistence of foetal haemoglobin (HPFH). Nucleic Acids Res. 1980 Apr 11;8(7):1521–1534. doi: 10.1093/nar/8.7.1521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
  7. Chovnick A., Gelbart W., McCarron M. Organization of the Rosy locus in Drosophila melanogaster. Cell. 1977 May;11(1):1–10. doi: 10.1016/0092-8674(77)90312-9. [DOI] [PubMed] [Google Scholar]
  8. Engels W. R. Estimating genetic divergence and genetic variability with restriction endonucleases. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6329–6333. doi: 10.1073/pnas.78.10.6329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Engels W. R. Hybrid dysgenesis in Drosophila and the Stochastic loss hypothesis. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):561–565. doi: 10.1101/sqb.1981.045.01.072. [DOI] [PubMed] [Google Scholar]
  10. Ewens W. J., Spielman R. S., Harris H. Estimation of genetic variation at the DNA level from restriction endonuclease data. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3748–3750. doi: 10.1073/pnas.78.6.3748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fritsch E. F., Lawn R. M., Maniatis T. Characterisation of deletions which affect the expression of fetal globin genes in man. Nature. 1979 Jun 14;279(5714):598–603. doi: 10.1038/279598a0. [DOI] [PubMed] [Google Scholar]
  12. Goldberg D. A. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794–5798. doi: 10.1073/pnas.77.10.5794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Green M. M. Transposable elements in Drosophila and other Diptera. Annu Rev Genet. 1980;14:109–120. doi: 10.1146/annurev.ge.14.120180.000545. [DOI] [PubMed] [Google Scholar]
  14. Higgs D. R., Goodbourn S. E., Wainscoat J. S., Clegg J. B., Weatherall D. J. Highly variable regions of DNA flank the human alpha globin genes. Nucleic Acids Res. 1981 Sep 11;9(17):4213–4224. doi: 10.1093/nar/9.17.4213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jeffreys A. J. DNA sequence variants in the G gamma-, A gamma-, delta- and beta-globin genes of man. Cell. 1979 Sep;18(1):1–10. doi: 10.1016/0092-8674(79)90348-9. [DOI] [PubMed] [Google Scholar]
  16. Judd B. H., Shen M. W., Kaufman T. C. The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics. 1972 May;71(1):139–156. doi: 10.1093/genetics/71.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kaplan N., Langley C. H. A new estimate of sequence divergence of mitochondrial DNA using restriction endonuclease mappings. J Mol Evol. 1979 Nov;13(4):295–304. doi: 10.1007/BF01731370. [DOI] [PubMed] [Google Scholar]
  18. Kaplan N., Risko K. An improved method for estimating sequence divergence of DNA using restriction endonuclease mappings. J Mol Evol. 1981;17(3):156–162. doi: 10.1007/BF01733909. [DOI] [PubMed] [Google Scholar]
  19. Laurie-Ahlberg C. C., Maroni G., Bewley G. C., Lucchesi J. C., Weir B. S. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073–1077. doi: 10.1073/pnas.77.2.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Leigh Brown A. J., Ish-Horowicz D. Evolution of the 87A and 87C heat-shock loci in Drosophila. Nature. 1981 Apr 23;290(5808):677–682. doi: 10.1038/290677a0. [DOI] [PubMed] [Google Scholar]
  21. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  22. Nasmyth K. A., Tatchell K., Hall B. D., Astell C., Smith M. Physical analysis of mating-type loci in Saccharomyces cerevisiae. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):961–981. doi: 10.1101/sqb.1981.045.01.113. [DOI] [PubMed] [Google Scholar]
  23. Nei M., Li W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269–5273. doi: 10.1073/pnas.76.10.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nei M., Tajima F. DNA polymorphism detectable by restriction endonucleases. Genetics. 1981 Jan;97(1):145–163. doi: 10.1093/genetics/97.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  26. Rotwein P., Chyn R., Chirgwin J., Cordell B., Goodman H. M., Permut M. A. Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes. Science. 1981 Sep 4;213(4512):1117–1120. doi: 10.1126/science.6267694. [DOI] [PubMed] [Google Scholar]
  27. Rubin G. M., Brorein W. J., Jr, Dunsmuir P., Flavell A. J., Levis R., Strobel E., Toole J. J., Young E. Copia-like transposable elements in the Drosophila genome. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):619–628. doi: 10.1101/sqb.1981.045.01.080. [DOI] [PubMed] [Google Scholar]
  28. Schaffer H. E., Sederoff R. R. Improved estimation of DNA fragment lengths from Agarose gels. Anal Biochem. 1981 Jul 15;115(1):113–122. doi: 10.1016/0003-2697(81)90533-9. [DOI] [PubMed] [Google Scholar]
  29. Shah D. M., Langley C. H. Inter- and intraspecific variation in restriction maps of Drosophila mitochondrial DNAs. Nature. 1979 Oct 25;281(5733):696–699. doi: 10.1038/281696a0. [DOI] [PubMed] [Google Scholar]
  30. Spradling A. C., Rubin G. M. Drosophila genome organization: conserved and dynamic aspects. Annu Rev Genet. 1981;15:219–264. doi: 10.1146/annurev.ge.15.120181.001251. [DOI] [PubMed] [Google Scholar]
  31. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Young M. W., Schwartz H. E. Nomadic gene families in Drosophila. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):629–640. doi: 10.1101/sqb.1981.045.01.081. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES