Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Oct;79(19):5818–5822. doi: 10.1073/pnas.79.19.5818

Nucleotide sequence of tobacco mosaic virus RNA.

P Goelet, G P Lomonossoff, P J Butler, M E Akam, M J Gait, J Karn
PMCID: PMC347001  PMID: 6964389

Abstract

Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral population. The positive strand coding sequence starts 69 nucleotides from the 5' end with a reading frame for a protein of Mr 125,941 and terminates with UAG. Readthrough of this terminator would give rise to a protein of Mr 183,253. Overlapping the terminal five codons of this readthrough reading frame is a second reading frame coding for a protein of Mr 29,987. This gene terminates two nucleotides before the initiator codon of the coat protein gene. Potential signal sequences responsible for the capping and synthesis of the coat protein and Mr 29,987 protein mRNAs have been identified. Similar sequences within these reading frames may be used in the expression of sets of proteins that share COOH-terminal sequences.

Full text

PDF
5820

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahlquist P., Luckow V., Kaesberg P. Complete nucleotide sequence of brome mosaic virus RNA3. J Mol Biol. 1981 Nov 25;153(1):23–38. doi: 10.1016/0022-2836(81)90524-6. [DOI] [PubMed] [Google Scholar]
  2. Beachy R. N., Zaitlin M. Characterization and in vitro translation of the RNAs from less-than-full-length, virus-related, nucleoprotein rods present in tobacco mosaic virus preparations. Virology. 1977 Aug;81(1):160–169. doi: 10.1016/0042-6822(77)90068-x. [DOI] [PubMed] [Google Scholar]
  3. Beaudet A. L., Caskey C. T. Mammalian peptide chain termination. II. Codon specificity and GTPase activity of release factor. Proc Natl Acad Sci U S A. 1971 Mar;68(3):619–624. doi: 10.1073/pnas.68.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Butler P. J., Lomonossoff G. P. Quantized incorporation of RNA during assembly of tobacco mosaic virus from protein disks. J Mol Biol. 1978 Dec 25;126(4):877–882. doi: 10.1016/0022-2836(78)90027-x. [DOI] [PubMed] [Google Scholar]
  5. Domingo E., Sabo D., Taniguchi T., Weissmann C. Nucleotide sequence heterogeneity of an RNA phage population. Cell. 1978 Apr;13(4):735–744. doi: 10.1016/0092-8674(78)90223-4. [DOI] [PubMed] [Google Scholar]
  6. Duckworth M. L., Gait M. J., Goelet P., Hong G. F., Singh M., Titmas R. C. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route. Nucleic Acids Res. 1981 Apr 10;9(7):1691–1706. doi: 10.1093/nar/9.7.1691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Engelberg-Kulka H. UGA suppression by normal tRNA Trp in Escherichia coli: codon context effects. Nucleic Acids Res. 1981 Feb 25;9(4):983–991. doi: 10.1093/nar/9.4.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gait M. J., Sheppard R. C. Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method. Nucleic Acids Res. 1977 Apr;4(4):1135–1158. doi: 10.1093/nar/4.4.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goelet P., Karn J. Tobacco mosaic virus induces the synthesis of a family of 3' coterminal messenger RNAs and their complements. J Mol Biol. 1982 Jan 25;154(3):541–550. doi: 10.1016/s0022-2836(82)80013-2. [DOI] [PubMed] [Google Scholar]
  10. Guilley H., Jonard G., Kukla B., Richards K. E. Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA. Nucleic Acids Res. 1979 Apr;6(4):1287–1308. doi: 10.1093/nar/6.4.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hunter T. R., Hunt T., Knowland J., Zimmern D. Messenger RNA for the coat protein of tobacco mosaic virus. Nature. 1976 Apr 29;260(5554):759–764. doi: 10.1038/260759a0. [DOI] [PubMed] [Google Scholar]
  12. Jonard G., Richards K., Mohier E., Gerlinger P. Nucleotide sequence at the 5' extremity of tobacco-mosaic-virus RNA. 2. The coding region (nucleotides 69-236). Eur J Biochem. 1978 Mar 15;84(2):521–531. doi: 10.1111/j.1432-1033.1978.tb12195.x. [DOI] [PubMed] [Google Scholar]
  13. Kohli J., Grosjean H. Usage of the three termination codons: compilation and analysis of the known eukaryotic and prokaryotic translation termination sequences. Mol Gen Genet. 1981;182(3):430–439. doi: 10.1007/BF00293932. [DOI] [PubMed] [Google Scholar]
  14. Koper-Zwarthoff E. C., Brederode F. T., Veeneman G., van Boom J. H., Bol J. F. Nucleotide sequences at the 5'-termini of the alfalfa mosaic virus RNAs and the intercistronic junction in RNA 3. Nucleic Acids Res. 1980 Dec 11;8(23):5635–5647. doi: 10.1093/nar/8.23.5635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981 Oct 24;9(20):5233–5252. doi: 10.1093/nar/9.20.5233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lomonossoff G. P., Butler P. J. Assembly of tobacco mosaic virus: elongation towards the 3'-hydroxyl terminus of the RNA. FEBS Lett. 1980 May 5;113(2):271–274. doi: 10.1016/0014-5793(80)80607-7. [DOI] [PubMed] [Google Scholar]
  17. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pelham H. R. Leaky UAG termination codon in tobacco mosaic virus RNA. Nature. 1978 Mar 30;272(5652):469–471. doi: 10.1038/272469a0. [DOI] [PubMed] [Google Scholar]
  19. Pelham H. R. Translation of fragmented viral RNA in vitro: initiation at multiple sites. FEBS Lett. 1979 Apr 1;100(1):195–199. doi: 10.1016/0014-5793(79)81162-x. [DOI] [PubMed] [Google Scholar]
  20. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  21. Shinnick T. M., Lerner R. A., Sutcliffe J. G. Nucleotide sequence of Moloney murine leukaemia virus. Nature. 1981 Oct 15;293(5833):543–548. doi: 10.1038/293543a0. [DOI] [PubMed] [Google Scholar]
  22. Staden R. A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Res. 1980 Aug 25;8(16):3673–3694. doi: 10.1093/nar/8.16.3673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Van Tol R. G., Van Gemeren R., Van Vloten-Doting L. Two leaky termination codons in AMV RNA 1. FEBS Lett. 1980 Aug 25;118(1):67–71. doi: 10.1016/0014-5793(80)81220-8. [DOI] [PubMed] [Google Scholar]
  24. Wickens M. P., Buell G. N., Schimke R. T. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I. J Biol Chem. 1978 Apr 10;253(7):2483–2495. [PubMed] [Google Scholar]
  25. Zimmern D. The nucleotide sequence at the origin for assembly on tobacco mosaic virus RNA. Cell. 1977 Jul;11(3):463–482. doi: 10.1016/0092-8674(77)90065-4. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES