Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Oct;79(20):6215–6219. doi: 10.1073/pnas.79.20.6215

Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA.

D Hatfield, A Diamond, B Dudock
PMCID: PMC347090  PMID: 6815648

Abstract

An unusual minor species of bovine liver serine tRNA has previously been isolated, sequenced, and found to suppress the UGA termination codon in protein synthesis in vitro [Diamond, A., Dudock, B. & Hatfield, D. (1981) Cell 25, 497-506]. We have now found that this tRNA can be a substrate in a specific phosphorylation reaction in which phosphoseryl-tRNA is formed. Moreover, bovine liver contains a second UGA suppressor serine tRNA (tRNASerNCA; N is a modified nucleoside) which also forms phosphoseryl-tRNA. The nucleotide sequence and coding properties of tRNASerNCA are presented.

Full text

PDF
6217

Images in this article

6218Image
on p.6218

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bogenhagen D. F., Sakonju S., Brown D. D. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region. Cell. 1980 Jan;19(1):27–35. doi: 10.1016/0092-8674(80)90385-2. [DOI] [PubMed] [Google Scholar]
  2. Diamond A., Dudock B., Hatfield D. Structure and properties of a bovine liver UGA suppressor serine tRNA with a tryptophan anticodon. Cell. 1981 Aug;25(2):497–506. doi: 10.1016/0092-8674(81)90068-4. [DOI] [PubMed] [Google Scholar]
  3. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fowlkes D. M., Shenk T. Transcriptional control regions of the adenovirus VAI RNA gene. Cell. 1980 Nov;22(2 Pt 2):405–413. doi: 10.1016/0092-8674(80)90351-7. [DOI] [PubMed] [Google Scholar]
  5. Galli G., Hofstetter H., Birnstiel M. L. Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements. Nature. 1981 Dec 17;294(5842):626–631. doi: 10.1038/294626a0. [DOI] [PubMed] [Google Scholar]
  6. Geller A. I., Rich A. A UGA termination suppression tRNATrp active in rabbit reticulocytes. Nature. 1980 Jan 3;283(5742):41–46. doi: 10.1038/283041a0. [DOI] [PubMed] [Google Scholar]
  7. Gupta R. C., Randerath K. Rapid print-readout technique for sequencing of RNA's containing modified nucleotides. Nucleic Acids Res. 1979 Aug 10;6(11):3443–3458. doi: 10.1093/nar/6.11.3443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hatfield D., Matthews C. R., Rice M. Aminoacyl-transfer RNA populations in mammalian cells chromatographic profiles and patterns of codon recognition. Biochim Biophys Acta. 1979 Oct 25;564(3):414–423. doi: 10.1016/0005-2787(79)90032-7. [DOI] [PubMed] [Google Scholar]
  9. Hatfield D. Recognition of nonsense codons in mammalian cells. Proc Natl Acad Sci U S A. 1972 Oct;69(10):3014–3018. doi: 10.1073/pnas.69.10.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hawthorne D. C. UGA mutations and UGA suppressors in yeast. Biochimie. 1976;58(1-2):179–182. doi: 10.1016/s0300-9084(76)80368-9. [DOI] [PubMed] [Google Scholar]
  11. Mäenpä P. H., Bernfield M. R. A specific hepatic transfer RNA for phosphoserine. Proc Natl Acad Sci U S A. 1970 Oct;67(2):688–695. doi: 10.1073/pnas.67.2.688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NIRENBERG M., LEDER P. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES. Science. 1964 Sep 25;145(3639):1399–1407. doi: 10.1126/science.145.3639.1399. [DOI] [PubMed] [Google Scholar]
  13. Sakonju S., Bogenhagen D. F., Brown D. D. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. Cell. 1980 Jan;19(1):13–25. doi: 10.1016/0092-8674(80)90384-0. [DOI] [PubMed] [Google Scholar]
  14. Sharp S. J., Stewart T. S. The characterization of phosphoseryl tRNA from lactating bovine mammary gland. Nucleic Acids Res. 1977 Jul;4(7):2123–2136. doi: 10.1093/nar/4.7.2123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Silberklang M., Gillum A. M., RajBhandary U. L. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs. Methods Enzymol. 1979;59:58–109. doi: 10.1016/0076-6879(79)59072-7. [DOI] [PubMed] [Google Scholar]
  16. Weiner A. M., Weber K. A single UGA codon functions as a natural termination signal in the coliphage q beta coat protein cistron. J Mol Biol. 1973 Nov 15;80(4):837–855. doi: 10.1016/0022-2836(73)90213-1. [DOI] [PubMed] [Google Scholar]
  17. Weiner A. M., Weber K. Natural read-through at the UGA termination signal of Q-beta coat protein cistron. Nat New Biol. 1971 Sep 15;234(50):206–209. doi: 10.1038/newbio234206a0. [DOI] [PubMed] [Google Scholar]
  18. Yates J. L., Gette W. R., Furth M. E., Nomura M. Effects of ribosomal mutations on the read-through of a chain termination signal: studies on the synthesis of bacteriophage lambda O gene protein in vitro. Proc Natl Acad Sci U S A. 1977 Feb;74(2):689–693. doi: 10.1073/pnas.74.2.689. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES