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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Oct;79(20):6304–6308. doi: 10.1073/pnas.79.20.6304

Translation of mRNAs for subunits of chloroplast coupling factor 1 in spinach

Akira Watanabe 1,*, Carl A Price 1
PMCID: PMC347109  PMID: 16593240

Abstract

The chloroplast coupling factor 1 consists of five nonidentical subunits, three of which (α, β, and ε subunits) have been shown in several laboratories to be synthesized within chloroplasts. The site of synthesis of the remaining two (γ and δ subunits) was investigated by analyzing products directed by spinach leaf RNAs in wheat germ and reticulocyte translation systems in vitro. It was found that poly(A)+ RNA directs the synthesis of two distinct polypeptides, one of which is immunochemically related to the γ subunit but is 4,000 daltons larger. The other shares antigenic sites with the δ subunit but is 8,000 daltons larger. When wheat germ or reticulocyte translation systems were programmed with RNAs from purified chloroplasts, the only products related to CF1 that we could detect were a putative precursor of β, 2,000 daltons larger than the mature subunit, and some smaller polypeptides, which appear to be incomplete translation products of β. From these results it appears likely that the γ and δ subunits are synthesized in the cytoplasm as larger precursors and that β is synthesized within the chloroplast as a precursor.

Keywords: plastid genes, nuclear genes, protein precursor

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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