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. 1982 Dec;79(24):7629–7633. doi: 10.1073/pnas.79.24.7629

Identification of a human neuroectodermal tumor antigen (OFA-I-2) as ganglioside GD2.

L D Cahan, R F Irie, R Singh, A Cassidenti, J C Paulson
PMCID: PMC347401  PMID: 6296843

Abstract

Two monospecific human antibodies (anti-OFA-I-1 and anti-OFA-I-2) produced in vitro by lymphoblast cell lines originating from melanoma patients have been shown previously to recognize cell surface antigens (OFA-I-1 and OFA-I-2) on human tumors and fetal brain: OFA-I-1 is expressed on a variety of human tumors, while OFA-I-2 has been detected only on tumors of neuroectodermal origin. Evidence presented in this report suggests that the two antigens expressed by a cultured human melanoma cell line (M14) are chemically distinct and that OFA-I-2 is a cell surface glycolipid, ganglioside GD2: GalNAc beta 1 leads to 4 NeuAc alpha 2 leads to 8NeuAc alpha 2 leads to 3 Gal beta 1 leads to 4Glc-ceramide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahn S. S., Irie R. F., Weisenburger T. H., Jones P. C., Juillard G., Roe D. J., Morton D. L. Humoral immune response to intralymphatic immunotherapy for disseminated melanoma: correlation with clinical response. Surgery. 1982 Aug;92(2):362–367. [PubMed] [Google Scholar]
  2. Ando S., Yu R. K. Isolation and characterization of two isomers of brain tetrasialogangliosides. J Biol Chem. 1979 Dec 10;254(23):12224–12229. [PubMed] [Google Scholar]
  3. Coleman P. L., Fishman P. H., Brady R. O., Todaro G. J. Altered ganglioside biosynthesis in mouse cell cultures following transformation with chemical carcinogens and x-irradiation. J Biol Chem. 1975 Jan 10;250(1):55–60. [PubMed] [Google Scholar]
  4. Glasgow L. R., Paulson J. C., Hill R. L. Systematic purification of five glycosidases from Streptococcus (Diplococcus) pneumoniae. J Biol Chem. 1977 Dec 10;252(23):8615–8623. [PubMed] [Google Scholar]
  5. Hakomori S. Glycosphingolipids in cellular interaction, differentiation, and oncogenesis. Annu Rev Biochem. 1981;50:733–764. doi: 10.1146/annurev.bi.50.070181.003505. [DOI] [PubMed] [Google Scholar]
  6. Hakomori S. Structures and organization of cell surface glycolipids dependency on cell growth and malignant transformation. Biochim Biophys Acta. 1975 Mar 20;417(1):55–89. doi: 10.1016/0304-419x(75)90008-6. [DOI] [PubMed] [Google Scholar]
  7. Irie K., Irie R. F., Morton D. L. Evidence for in vivo reaction of antibody and complement to surface antigens of human cancer cells. Science. 1974 Nov 1;186(4162):454–456. doi: 10.1126/science.186.4162.454. [DOI] [PubMed] [Google Scholar]
  8. Irie R. F., Giuliano A. E., Morton D. L. Oncofetal antigen: a tumor-associated fetal antigen immunogenic in man. J Natl Cancer Inst. 1979 Aug;63(2):367–373. [PubMed] [Google Scholar]
  9. Irie R. F., Irie K., Morton D. L. A membrane antigen common to human cancer and fetal brain tissues. Cancer Res. 1976 Sep;36(9 Pt 2):3510–3517. [PubMed] [Google Scholar]
  10. Irie R. F., Jones P. C., Morton D. L., Sidell N. In vitro production of human antibody to a tumour-associated foetal antigen. Br J Cancer. 1981 Aug;44(2):262–266. doi: 10.1038/bjc.1981.178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Irie R. F., Sze L. L., Saxton R. E. Human antibody to OFA-I, a tumor antigen, produced in vitro by Epstein-Barr virus-transformed human B-lymphoid cell lines. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5666–5670. doi: 10.1073/pnas.79.18.5666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones P. C., Sze L. L., Liu P. Y., Morton D. L., Irie R. F. Prolonged survival for melanoma patients with elevated IgM antibody to oncofetal antigen. J Natl Cancer Inst. 1981 Feb;66(2):249–254. [PubMed] [Google Scholar]
  13. Klein G., Clifford P., Klein E., Stjernswärd J. Search for tumor-specific immune reactions in Burkitt lymphoma patients by the membrane immunofluorescence reaction. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1628–1635. doi: 10.1073/pnas.55.6.1628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Klenk E., Naoi M. Uber eine Komponente des Gemisches der Gehirnganglioside, die durch Neuraminidaseeinwirkung in das Tay-Sachs-Gangliosid übergeht. Hoppe Seylers Z Physiol Chem. 1968 Mar;349(3):288–292. [PubMed] [Google Scholar]
  15. Lewis M. G. Possible immunological factors in human malignant melanoma in Uganda. Lancet. 1967 Oct 28;2(7522):921–922. doi: 10.1016/s0140-6736(67)90236-x. [DOI] [PubMed] [Google Scholar]
  16. Magnani J. L., Brockhaus M., Smith D. F., Ginsburg V., Blaszczyk M., Mitchell K. F., Steplewski Z., Koprowski H. A monosialoganglioside is a monoclonal antibody-defined antigen of colon carcinoma. Science. 1981 Apr 3;212(4490):55–56. doi: 10.1126/science.7209516. [DOI] [PubMed] [Google Scholar]
  17. Portoukalian J., Zwingelstein G., Doré J. F. Lipid composition of human malignant melanoma tumors at various levels of malignant growth. Eur J Biochem. 1979 Feb 15;94(1):19–23. doi: 10.1111/j.1432-1033.1979.tb12866.x. [DOI] [PubMed] [Google Scholar]
  18. Pukel C. S., Lloyd K. O., Travassos L. R., Dippold W. G., Oettgen H. F., Old L. J. GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody. J Exp Med. 1982 Apr 1;155(4):1133–1147. doi: 10.1084/jem.155.4.1133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SVENNERHOLM L. CHROMATOGRAPHIC SEPARATION OF HUMAN BRAIN GANGLIOSIDES. J Neurochem. 1963 Sep;10:613–623. doi: 10.1111/j.1471-4159.1963.tb08933.x. [DOI] [PubMed] [Google Scholar]
  20. SVENNERHOLM L. The quantitative estimation of cerebrosides in nervous tissue. J Neurochem. 1956 May;1(1):42–53. doi: 10.1111/j.1471-4159.1956.tb12053.x. [DOI] [PubMed] [Google Scholar]
  21. Schauer R., Buscher H. P., Casals-Stenzel J. Sialic acids: their analysis and enzymic modification in relation to the synthesis of submandibular-gland glycoproteins. Biochem Soc Symp. 1974;(40):87–116. [PubMed] [Google Scholar]
  22. Shiku H., Takahashi T., Oettgen H. F. Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens. J Exp Med. 1976 Oct 1;144(4):873–881. doi: 10.1084/jem.144.4.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sidell N., Irie R. F., Morton D. L. Oncofoetal antigen I: a target for immune cytolysis of human cancer. Br J Cancer. 1979 Dec;40(6):950–953. doi: 10.1038/bjc.1979.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Springer G. F., Desai P. R., Scanlon E. F. Blood group MN precursors as human breast carcinoma-associated antigens and "naturally" occurring human cytotoxins against them. Cancer. 1976 Jan;37(1):169–176. doi: 10.1002/1097-0142(197601)37:1<169::aid-cncr2820370124>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  25. Sundsmo J. S., Hakomori S. Lacto-N-neotetraosylceramide ("paragloboside") as a possible tumor-associated surface antigen of hamster NILPY tumor. Biochem Biophys Res Commun. 1976 Feb 9;68(3):799–806. doi: 10.1016/0006-291x(76)91216-x. [DOI] [PubMed] [Google Scholar]
  26. Svennerholm L., Fredman P. A procedure for the quantitative isolation of brain gangliosides. Biochim Biophys Acta. 1980 Jan 18;617(1):97–109. doi: 10.1016/0005-2760(80)90227-1. [DOI] [PubMed] [Google Scholar]
  27. Svennerholm L., Vanier M. T., Månsson J. E. Krabbe disease: a galactosylsphingosine (psychosine) lipidosis. J Lipid Res. 1980 Jan;21(1):53–64. [PubMed] [Google Scholar]
  28. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  29. WELLS M. A., DITTMER J. C. THE USE OF SEPHADEX FOR THE REMOVAL OF NONLIPID CONTAMINANTS FROM LIPID EXTRACTS. Biochemistry. 1963 Nov-Dec;2:1259–1263. doi: 10.1021/bi00906a015. [DOI] [PubMed] [Google Scholar]
  30. Wenger D. A., Wardell S. Action of neuraminidase (EC 3.2.1.18) from Clostridium perfringens on brain gangliosides in the presence of bile salts. J Neurochem. 1973 Feb;20(2):607–612. doi: 10.1111/j.1471-4159.1973.tb12159.x. [DOI] [PubMed] [Google Scholar]
  31. Wu P. S., Ledeen R. W., Udem S., Isaacson Y. A. Nature of the Sendai virus receptor: glycoprotein versus ganglioside. J Virol. 1980 Jan;33(1):304–310. doi: 10.1128/jvi.33.1.304-310.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Yeh M. Y., Hellström I., Abe K., Hakomori S., Hellström K. E. A cell-surface antigen which is present in the ganglioside fraction and shared by human melanomas. Int J Cancer. 1982 Mar 15;29(3):269–275. doi: 10.1002/ijc.2910290308. [DOI] [PubMed] [Google Scholar]

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