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. 2006 May 20;50(1-3):109–119. doi: 10.1007/s10616-006-9007-6

Current status of transcriptional regulation systems

Tobias May 1, Hansjörg Hauser 1,✉, Dagmar Wirth 1
PMCID: PMC3476005  PMID: 19003074

Abstract

Many attempts have been undertaken to control transgene activity in mammalian cells. This is of importance for both applied biotechnology and basic research activities. State of the art regulatory systems use elements for transgene regulation which are unrelated to host regulatory networks and thus do not interfere with endogenous activities. Most of these regulation systems consist of transregulators and transregulator responding promoter elements that are derived from non mammalian origin. Apart from the tetracycline (Tet) regulated system which is most widely used for conditional gene expression at the moment, a number of new systems were created. These systems have been significantly refined and their performance makes them suitable for regulating transgenes not only in cellular systems but also in transgenic animals and for human therapeutic use.

Keywords: Viral transduction, Autoregulatory expression, Test-system, Synthetic promotor, Gene regulation

Abbreviations

eGFP

 Enhanced green fluorescent protein

EPO

 Recombinant human erythropoietin

GOI

 Gene of interest

IRES

 Internal ribosomal entry site

lmw

 Low molecular weight

pA

 Polyadenylation site

PTA

 Transactivator dependent promoter

SEAP

 Secreted form of human placental alkaline phosphatase

TAg

 Simian virus 40 large T antigen

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