Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Nov;38(2):604–609. doi: 10.1128/iai.38.2.604-609.1982

Genomic and phenotypic analyses of Mycoplasma pneumoniae strains.

D K Chandler, S Razin, E B Stephens, R Harasawa, M F Barile
PMCID: PMC347782  PMID: 6183217

Abstract

Mycoplasma pneumoniae strains PI-1428, M129, B176, FH, and MAC were analyzed for homology by DNA-DNA hybridization, thermal elution midpoints of the DNA-DNA heteroduplexes, DNA cleavage patterns with restriction endonucleases, and protein gel electrophoretic profiles. These properties were compared with biological assays, such as antigenic reactivity with reference antisera in the growth inhibition and metabolic inhibition tests, as well as the ability to attach to human WiDr cell cultures. The avirulent, nonhemadsorbing strain B176 could be differentiated from the others by diminished attachment capacity to WiDr cells and by slightly reduced DNA homology. The other strains appeared to be identical when examined by these procedures. No significant differences were detected among any of the strains by the metabolic inhibition or growth inhibition tests. The DNA cleavage patterns of the five strains were also similar, although strains FH and MAC differed from the other three strains in a few bands. These results demonstrate that these five strains are similar and that the species M. pneumoniae is remarkably homogeneous.

Full text

PDF
607

Images in this article

606Image
on p.606

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aulakh G. S., Gallo R. C. Rauscher-leukemia-virus-related sequences in human DNA: presence in some tissues of some patients with hemotopoietic neoplasias and absence in DNA from other tissues. Proc Natl Acad Sci U S A. 1977 Jan;74(1):353–357. doi: 10.1073/pnas.74.1.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barile M. F., Chandler D. K., Yoshida H., Grabowski M. W., Harasawa R., Ahmed O. A. Hamster challenge potency assay for evaluation of Mycoplasma pneumoniae vaccines. Isr J Med Sci. 1981 Jul;17(7):682–686. [PubMed] [Google Scholar]
  3. CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
  4. Chandler D. K., Barile M. F. Ciliostatic, hemagglutinating, and proteolytic activities in a cell extract of Mycoplasma pneumoniae. Infect Immun. 1980 Sep;29(3):1111–1116. doi: 10.1128/iai.29.3.1111-1116.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chandler D. K., Collier A. M., Barile M. F. Attachment of Mycoplasma pneumoniae to hamster tracheal organ cultures, tracheal outgrowth monolayers, human erythrocytes, and WiDr human tissue culture cells. Infect Immun. 1982 Mar;35(3):937–942. doi: 10.1128/iai.35.3.937-942.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hansen E. J., Wilson R. M., Baseman J. B. Two-dimensional gel electrophoretic comparison of proteins from virulent and avirulent strains of Mycoplasma pneumoniae. Infect Immun. 1979 May;24(2):468–475. doi: 10.1128/iai.24.2.468-475.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hayatsu E. Acquired immunity to Mycoplasma pneumoniae. Pneumonia in hamsters. Microbiol Immunol. 1978;22(4):181–195. doi: 10.1111/j.1348-0421.1978.tb00362.x. [DOI] [PubMed] [Google Scholar]
  8. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  9. Hu P. C., Cole R. M., Huang Y. S., Graham J. A., Gardner D. E., Collier A. M., Clyde W. A., Jr Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 1982 Apr 16;216(4543):313–315. doi: 10.1126/science.6801766. [DOI] [PubMed] [Google Scholar]
  10. Hu P. C., Collier A. M., Baseman J. B. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J Exp Med. 1977 May 1;145(5):1328–1343. doi: 10.1084/jem.145.5.1328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kelly R. B., Cozzarelli N. R., Deutscher M. P., Lehman I. R., Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. J Biol Chem. 1970 Jan 10;245(1):39–45. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Laird C. D., McConaughy B. L., McCarthy B. J. Rate of fixation of nucleotide substitutions in evolution. Nature. 1969 Oct 11;224(5215):149–154. doi: 10.1038/224149a0. [DOI] [PubMed] [Google Scholar]
  15. Lipman R. P., Clyde W. A., Jr, Denny F. W. Characteristics of virulent, attenuated, and avirulent Mycoplasma pneumoniae strains. J Bacteriol. 1969 Nov;100(2):1037–1043. doi: 10.1128/jb.100.2.1037-1043.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lipman R. P., Clyde W. A., Jr The interrelationship of virulence, cytadsorption, and peroxide formation in Mycoplasma pneumoniae. Proc Soc Exp Biol Med. 1969 Sep;131(4):1163–1167. doi: 10.3181/00379727-131-34061. [DOI] [PubMed] [Google Scholar]
  17. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mouches C., Taylor-Robinson D., Stipkovits L., Bove J. M. Comparison of human and animal Ureaplasmas by one- and two-dimensional protein analysis on polyacrylamide slab gel. Ann Microbiol (Paris) 1981 Sep-Oct;132B(2):171–196. [PubMed] [Google Scholar]
  19. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES