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. 1982 Dec;38(3):1003–1009. doi: 10.1128/iai.38.3.1003-1009.1982

Comparison of systemic and local immunity in dogs with canine parvovirus gastroenteritis.

J B Rice, K A Winters, S Krakowka, R G Olsen
PMCID: PMC347849  PMID: 7152659

Abstract

To determine whether resistance to canine parvovirus (CPV) gastroenteritis is mediated by local or systemic immunity or both, an enzyme-linked immunospecific antibody assay (ELISA) was developed that quantitated different classes of antibody to CPV. Antibody levels in serum and feces of dogs with CPV-associated gastroenteritis were compared with their clinical signs and viral hemagglutination (HA) titers. Dogs with high levels of CPV coproantibody had a favorable clinical prognosis, high serum antibody levels (hemagglutination inhibition [HI] and ELISA), and low viral HA titers in feces. Conversely, dogs with little or no detectable CPV coproantibody had severe clinical signs and associated mortality rates and high viral HA titers in feces. Many of these dogs had high HI antibody titers. Statistical analysis revealed that only coproantibody level correlated (inversely) with HA titer; serum antibody, whether measured by HI or ELISA, did not. These data suggest that local intestinal immunity is more important than humoral immunity in developing immunological resistance to CPV gastroenteritis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appel M. J., Scott F. W., Carmichael L. E. Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet Rec. 1979 Aug 25;105(8):156–159. doi: 10.1136/vr.105.8.156. [DOI] [PubMed] [Google Scholar]
  2. Bienenstock J., Befus A. D. Mucosal immunology. Immunology. 1980 Oct;41(2):249–270. [PMC free article] [PubMed] [Google Scholar]
  3. Blacklow N. R., Cukor G., Bedigian M. K., Echeverria P., Greenberg H. B., Schreiber D. S., Trier J. S. Immune response and prevalence of antibody to Norwalk enteritis virus as determined by radioimmunoassay. J Clin Microbiol. 1979 Dec;10(6):903–909. doi: 10.1128/jcm.10.6.903-909.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blacklow N. R., Cukor G. Viral gastroenteritis. N Engl J Med. 1981 Feb 12;304(7):397–406. doi: 10.1056/NEJM198102123040705. [DOI] [PubMed] [Google Scholar]
  5. Carmichael L. E., Joubert J. C., Pollock R. V. Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. Am J Vet Res. 1980 May;41(5):784–791. [PubMed] [Google Scholar]
  6. Haneberg B., Tonder O. Immunoglobulins and other serum proteins in feces from infants and children. Scand J Immunol. 1973;2(4):375–383. doi: 10.1111/j.1365-3083.1973.tb02046.x. [DOI] [PubMed] [Google Scholar]
  7. Kramer J. M., Meunier P. C., Pollock R. V. Canine parvovirus: update. Vet Med Small Anim Clin. 1980 Oct;75(10):1541–1555. [PubMed] [Google Scholar]
  8. McDermott M. R., Bienenstock J. Evidence for a common mucosal immunologic system. I. Migration of B immunoblasts into intestinal, respiratory, and genital tissues. J Immunol. 1979 May;122(5):1892–1898. [PubMed] [Google Scholar]
  9. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  10. Parrino T. A., Schreiber D. S., Trier J. S., Kapikian A. Z., Blacklow N. R. Clinical immunity in acute gastroenteritis caused by Norwalk agent. N Engl J Med. 1977 Jul 14;297(2):86–89. doi: 10.1056/NEJM197707142970204. [DOI] [PubMed] [Google Scholar]
  11. Rice J. B., Olsen R. G. Feline oncovirus-associated cell membrane antigen and feline leukemia virus group-specific antigen expression in bone marrow and serum. J Natl Cancer Inst. 1981 Apr;66(4):737–743. [PubMed] [Google Scholar]
  12. Waldman R. H., Ganguly R. Immunity to infections on secretory surfaces. J Infect Dis. 1974 Oct;130(4):419–440. doi: 10.1093/infdis/130.4.419. [DOI] [PubMed] [Google Scholar]
  13. Walker W. A., Isselbacher K. J. Intestinal antibodies. N Engl J Med. 1977 Oct 6;297(14):767–773. doi: 10.1056/NEJM197710062971407. [DOI] [PubMed] [Google Scholar]
  14. Welliver R. C., Ogra P. L. Importance of local immunity in enteric infection. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 2):560–564. [PubMed] [Google Scholar]

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