Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1983 Mar;39(3):1220–1227. doi: 10.1128/iai.39.3.1220-1227.1983

Molecular factors associated with virulence of marine vibrios isolated from striped bass in Chesapeake Bay.

A E Toranzo, J L Barja, S A Potter, R R Colwell, F M Hetrick, J H Crosa
PMCID: PMC348087  PMID: 6840838

Abstract

On the basis of cultural and biochemical properties as well as DNA homology assays, 81 Vibrio strains isolated from diseased striped bass and from Chesapeake Bay water were assigned to eight distinct groups. All organisms belonging to two of the groups were pathogenic for striped bass and were identified as Vibrio anguillarum, whereas organisms classified in the other six groups were nonpathogenic and were designated as Vibrio spp. Unlike the pathogenic V. anguillarum strain 775 isolated in the Pacific Northwest, strains pathogenic for striped bass did not contain any plasmids; however, they were similar to the Northwest isolates in that virulence was correlated with their ability to grow in the presence of nonimmune striped bass serum or under conditions of iron limitation. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of outer membranes showed that additional proteins were induced in those organisms capable of growth under conditions of iron limitation. It was of interest that 22 of the nonpathogenic isolates harbored one or more plasmids which, by restriction endonuclease analyses, were shown to be clearly different from the virulence plasmid pJM1.

Full text

PDF
1221

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowser P. R., Rosemark R., Reiner C. R. A preliminary report of vibriosis in cultured American lobsters, Homarus americanus. J Invertebr Pathol. 1981 Jan;37(1):80–85. doi: 10.1016/0022-2011(81)90058-6. [DOI] [PubMed] [Google Scholar]
  2. Brenner D. J., Fanning G. R., Johnson K. E., Citarella R. V., Falkow S. Polynucleotide sequence relationships among members of Enterobacteriaceae. J Bacteriol. 1969 May;98(2):637–650. doi: 10.1128/jb.98.2.637-650.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bullen J. J., Rogers H. J., Griffiths E. Role of iron in bacterial infection. Curr Top Microbiol Immunol. 1978;80:1–35. doi: 10.1007/978-3-642-66956-9_1. [DOI] [PubMed] [Google Scholar]
  4. Carruthers M. M., Kabat W. J. Vibrio vulnificus (lactose-positive vibrio) and Vibrio parahaemolyticus differ in their susceptibilities to human serum. Infect Immun. 1981 May;32(2):964–966. doi: 10.1128/iai.32.2.964-966.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crosa J. H. A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies an iron-sequestering system. Nature. 1980 Apr 10;284(5756):566–568. doi: 10.1038/284566a0. [DOI] [PubMed] [Google Scholar]
  6. Crosa J. H., Brenner D. J., Falkow S. Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J Bacteriol. 1973 Sep;115(3):904–911. doi: 10.1128/jb.115.3.904-911.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crosa J. H., Hodges L. L. Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum 775. Infect Immun. 1981 Jan;31(1):223–227. doi: 10.1128/iai.31.1.223-227.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crosa J. H., Hodges L. L., Schiewe M. H. Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum. Infect Immun. 1980 Mar;27(3):897–902. doi: 10.1128/iai.27.3.897-902.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Crosa J. H., Schiewe M. H., Falkow S. Evidence for plasmid contribution to the virulence of fish pathogen Vibrio anguillarum. Infect Immun. 1977 Nov;18(2):509–513. doi: 10.1128/iai.18.2.509-513.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DiSalvo L. H., Blecka J., Zebal R. Vibrio anguillarum and larval mortality in a California coastal shellfish hatchery. Appl Environ Microbiol. 1978 Jan;35(1):219–221. doi: 10.1128/aem.35.1.219-221.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hansen J. B., Olsen R. H. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5. J Bacteriol. 1978 Jul;135(1):227–238. doi: 10.1128/jb.135.1.227-238.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kiehn E. D., Pacha R. E. Characterization and relatedness of marine vibrios pathogenic to fish: deoxyribonucleic acid homology and base composition. J Bacteriol. 1969 Dec;100(3):1248–1255. doi: 10.1128/jb.100.3.1248-1255.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levin M. A., Wolke R. E., Cabelli V. J. Vibrio anguillarum as a cause of disease in winter flounder (Pseudopleuronectes americanus). Can J Microbiol. 1972 Oct;18(10):1585–1592. doi: 10.1139/m72-242. [DOI] [PubMed] [Google Scholar]
  15. Mittal K. R., Lalonde G., Leblanc D., Olivier G., Lallier R. Aeromonas hydrophila in rainbow trout: relation between virulence and surface characteristics. Can J Microbiol. 1980 Dec;26(12):1501–1503. doi: 10.1139/m80-248. [DOI] [PubMed] [Google Scholar]
  16. Neilands J. B. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. doi: 10.1146/annurev.bi.50.070181.003435. [DOI] [PubMed] [Google Scholar]
  17. Schiewe M. H., Crosa J. H., Ordal E. J. Deoxyribonucleic acid relationships among marine vibrios pathogenic to fish. Can J Microbiol. 1977 Aug;23(8):954–958. doi: 10.1139/m77-142. [DOI] [PubMed] [Google Scholar]
  18. Trust T. J., Courtice I. D., Khouri A. G., Crosa J. H., Schiewe M. H. Serum resistance and hemagglutination ability of marine vibrios pathogenic for fish. Infect Immun. 1981 Dec;34(3):702–707. doi: 10.1128/iai.34.3.702-707.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tubiash H. S., Colwell R. R., Sakazaki R. Marine vibrios associated with bacillary necrosis, a disease of larval and juvenile bivalve mollusks. J Bacteriol. 1970 Jul;103(1):271–272. doi: 10.1128/jb.103.1.271-272.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weinberg E. D. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. doi: 10.1128/mr.42.1.45-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES