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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Jan;77(1):629–633. doi: 10.1073/pnas.77.1.629

Presynaptic membrane potential affects transmitter release in an identified neuron in Aplysia by modulating the Ca2+ and K+ currents

Eli Shapiro 1,2, Vincent F Castellucci 1,2, Eric R Kandel 1,2
PMCID: PMC348328  PMID: 6244571

Abstract

We have examined the relationships between the modulation of transmitter release and of specific ionic currents by membrane potential in the cholinergic interneuron L10 of the abdominal ganglion of Aplysia californica. The presynaptic cell body was voltage-clamped under various pharmacological conditions and transmitter release from the terminals was assayed simultaneously by recording the synaptic potentials in the postsynaptic cell. When cell L10 was voltage-clamped from a holding potential of -60 mV in the presence of tetrodotoxin, graded transmitter release was evoked by depolarizing command pulses in the membrane voltage range (-35 mV to + 10 mV) in which the Ca2+ current was also increasing. Depolarizing the holding potential of L10 results in increased transmitter output. Two ionic mechanisms contribute to this form of plasticity. First, depolarization inactivates some K+ channels so that depolarizing command pulses recruit a smaller K+ current. In unclamped cells the decreased K+ conductance causes spike-broadening and increased influx of Ca2+ during each spike. Second, small depolarizations around resting potential (-55 mV to -35 mV) activate a steady-state Ca2+ current that also contributes to the modulation of transmitter release, because, even with most presynaptic K+ currents blocked pharmacologically, depolarizing the holding potential still increases transmitter release. In contrast to the steady-state Ca2+ current, the transient inward Ca2+ current evoked by depolarizing clamp steps is relatively unchanged from various holding potentials.

Keywords: ionic current, voltage clamp, synaptic transmission

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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