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. 1980 Feb;77(2):975–979. doi: 10.1073/pnas.77.2.975

Integrated and free viral DNA in hamster tumors induced by BK virus.

N Chenciner, G Meneguzzi, A Corallini, M P Grossi, P Grassi, G Barbanti-Brodano, G Milanesi
PMCID: PMC348406  PMID: 6244594

Abstract

BK virus (BKV)-induced tumors in hamsters were investigated for the presence of viral DNA by the blot-transfer hybridization technique. Several viral genomes per cell were found in tumor tissues and in their derived cell lines and clones. Most of the detected viral genomes were integrated into the cellular DNA, but some tumors also contained free viral DNA sequences. Integration patterns were different from each other, and many different integration sites were available on the cellular or on the viral DNA or on both. Typical features of integration patterns were found in ependymomas, which were the most frequent (72%) among BKV-induced tumors. Readily detectable viral DNA sequences were only found in neoplastic tissues, but traces of BKV DNA were also present in the apparently normal portion of the brain of an animal that had developed an ependymoma and in the brain (but not in the liver) of another animal 15 days after virus inoculation. A cell line and a single-cell clone derived from a tumor had hybridization patterns considerably simpler that the pattern of the original tumor, lacking several integrated viral genomes and all free viral sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birg F., Dulbecco R., Fried M., Kamen R. State and organization of polyoma virus DNA sequences in transformed rat cell lines. J Virol. 1979 Feb;29(2):633–648. doi: 10.1128/jvi.29.2.633-648.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Botchan M., Topp W., Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. doi: 10.1016/0092-8674(76)90118-5. [DOI] [PubMed] [Google Scholar]
  3. Campo M. S., Cameron I. R., Rogers M. E. Tandem integration of complete and defective SV40 genomes in mouse-human somatic cell hybrids. Cell. 1978 Dec;15(4):1411–1426. doi: 10.1016/0092-8674(78)90065-x. [DOI] [PubMed] [Google Scholar]
  4. Corallini A., Altavilla G., Cecchetti M. G., Fabris G., Grossi M. P., Balboni P. G., Lanza G., Barbanti-Brodano G. Ependymomas, malignant tumors of pancreatic islets, and osteosarcomas induced in hamsters by BK virus, a human papovavirus. J Natl Cancer Inst. 1978 Sep;61(3):875–883. [PubMed] [Google Scholar]
  5. Corallini A., Barbanti-Brodano G., Bortoloni W., Nenci I., Cassai E., Tampieri M., Portolani M., Borgatti M. High incidence of ependymomas induced by BK virus, a human papovavirus: brief communication. J Natl Cancer Inst. 1977 Nov;59(5):1561–1564. doi: 10.1093/jnci/59.5.1561. [DOI] [PubMed] [Google Scholar]
  6. Dhar R., Lai C. J., Khoury G. Nucleotide sequence of the DNA replication origin for human papovavirus BKV: sequence and structural homology with SV40. Cell. 1978 Feb;13(2):345–358. doi: 10.1016/0092-8674(78)90203-9. [DOI] [PubMed] [Google Scholar]
  7. Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
  8. Howley P. M., Khoury G., Byrne J. C., Takemoto K. K., Martin M. A. Physical map of the BK virus genome. J Virol. 1975 Oct;16(4):959–973. doi: 10.1128/jvi.16.4.959-973.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ketner G., Kelly T. J., Jr Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102–1106. doi: 10.1073/pnas.73.4.1102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Khoury G., Howley P. M., Garon C., Mullarkey M. F., Takemoto K. K., Martin M. A. Homology and relationship between the genomes of papovaviruses, BK virus and simian virus 40. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2563–2567. doi: 10.1073/pnas.72.7.2563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Laskey R. A., Mills A. D. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977 Oct 15;82(2):314–316. doi: 10.1016/0014-5793(77)80609-1. [DOI] [PubMed] [Google Scholar]
  12. Major E. O., Di Mayorca G. Malignant transformation of BHK21 clone 13 cells by BK virus--a human papovavirus. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3210–3212. doi: 10.1073/pnas.70.11.3210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meneguzzi G., Pignatti P. F., Barbanti-Brodano G., Milanesi G. Minichromosome from BK virus as a template for transcription in vitro. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1126–1130. doi: 10.1073/pnas.75.3.1126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mullarkey M. F., Hruska J. F., Takemoto K. K. Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis. J Virol. 1974 May;13(5):1014–1019. doi: 10.1128/jvi.13.5.1014-1019.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Näse L. M., Kärkkäinen M., Mäntyjärvi R. A. Transplantable hamster tumors induced with the BK virus. Acta Pathol Microbiol Scand B. 1975 Aug;83(4):347–352. doi: 10.1111/j.1699-0463.1975.tb00112.x. [DOI] [PubMed] [Google Scholar]
  16. Penney J. B., Jr, Narayan O. Studies of the antigenic relationships of the new human papovaviruses by electron microscopy agglutination. Infect Immun. 1973 Aug;8(2):299–300. doi: 10.1128/iai.8.2.299-300.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Portolani M., Barbanti-Brodano G., Placa M. L. Malignant transformation of hamster kidney cells by BK virus. J Virol. 1975 Feb;15(2):420–422. doi: 10.1128/jvi.15.2.420-422.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shah K. V., Daniel R. W., Strandberg J. D. Sarcoma in a hamster inoculated with BK virus, a human papovavirus. J Natl Cancer Inst. 1975 Apr;54(4):945–950. [PubMed] [Google Scholar]
  19. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Takemoto K. K., Mullarkey M. F. Human papovavirus, BK strain: biological studies including antigenic relationship to simian virus 40. J Virol. 1973 Sep;12(3):625–631. doi: 10.1128/jvi.12.3.625-631.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yang R. C., Wu R. BK virus DNA: cleavage map and sequence analysis. Proc Natl Acad Sci U S A. 1978 May;75(5):2150–2154. doi: 10.1073/pnas.75.5.2150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zouzias D., Prasad I., Basilico C. State of the viral DNA in rat cells transformed by polyma virus. II. Identification of the cells containing nonintegrated viral DNA and the effect of viral mutations. J Virol. 1977 Oct;24(1):142–150. doi: 10.1128/jvi.24.1.142-150.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. van der Noordaa J. Infectivity, oncogenicity and transforming ability of BK virus and BK virus DNA. J Gen Virol. 1976 Mar;30(3):371–373. doi: 10.1099/0022-1317-30-3-371. [DOI] [PubMed] [Google Scholar]

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