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. 1981 Sep;78(9):5416–5420. doi: 10.1073/pnas.78.9.5416

Cloning of bacteriophage fd gene 2 and construction of a plasmid dependent on fd gene 2 protein.

T F Meyer, K Geider
PMCID: PMC348756  PMID: 6272301

Abstract

Bacteriophage fd gene 2 was cloned in plasmid pBR325. Cells carrying the hybrid plasmid produce about 200 times more enzymatically active fd gene 2 protein than did cells infected with phage fd wild type, as measured by replication of phage fd replicative form I in vitro. Cloned gene 2 supports replication of an artificial phage fd miniplasmid consisting of the origin of bacteriophage fd replication and a gene coding for kanamycin resistance. This plasmid occurs in high copy numbers and is viable only in cells carrying the cloned fd gene 2 or in cells infected with phage fd. Because the miniplasmid is not propagated in natural hosts, it can be considered a safe cloning vector. Its fusion with the gene 2 hybrid plasmid provides an autonomous replicon independent of the polA function of the host cell. fd gene 2 is the only phage-encoded trans-acting function required for replication of double-stranded fd DNA in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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