Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Sep;78(9):5802–5806. doi: 10.1073/pnas.78.9.5802

Nucleotide sequence of constant and 3' untranslated regions of a kappa immunoglobulin light chain mRNA of a homozygous b4 rabbit.

O Heidmann, C Auffray, P A Cazenave, F Rougeon
PMCID: PMC348868  PMID: 6795636

Abstract

A homozygous a2/a2 and b4/b4 rabbit has been hyperimmunized with Micrococcus lysodeikticus. Poly(A)-containing RNA has been isolated from the spleen and translated in vitro, and translation products have been analyzed by NaDodSO4/polyacrylamide gel electrophoresis. Double-stranded cDNA has been synthesized from poly(A)-containing RNA template and inserted in the Pst I endonuclease site of plasmid pBR322 by using the oligo(dC).oligo(dG) tailing procedure. Tetracycline-resistant ampicillin-sensitive clones containing cDNA complementary to a kappa light chain mRNA have been selected by differential screening and their ability to hybridize to a spleen mRNA having the same size as a mouse kappa light chain mRNA. Two clones, pRk-15 and pRk-32, have been selected to determine the nucleotide sequence of the constant and 3' untranslated regions of kappa light chain mRNA, by the Maxam and Gilbert partial degradation method. Comparison of homologous regions of mouse kappa chain mRNA and b4 rabbit kappa chain mRNA reveals 61% homology in the constant region and 59% homology in the 3' untranslated region.

Full text

PDF
5803

Images in this article

5803Image
on p.5803
5803Image
on p.5803
5803Image
on p.5803

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appella E., Roholt O. A., Chersi A., Radzimski G., Pressman D. Amino acid sequence of the light chain derived from a rabbit anti-p-azobenzoate antibody of restricted heterogeneity. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1122–1129. doi: 10.1016/0006-291x(73)90581-0. [DOI] [PubMed] [Google Scholar]
  3. Auffray C., Nageotte R., Chambraud B., Rougeon F. Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain. Nucleic Acids Res. 1980 Mar 25;8(6):1231–1241. doi: 10.1093/nar/8.6.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  5. Brezin C., Cazenave P. A. La réaction croisee entre le motif allotypique Aa1 des immunoglobulines du lapin et les anticorps dirigés contre le motif allotypique Aa3: participation des variantes de la spécificité Aa1 à cette réaction croiśee. Immunochemistry. 1975 Mar;12(3):241–247. doi: 10.1016/0019-2791(75)90238-4. [DOI] [PubMed] [Google Scholar]
  6. Chang L. M., Bollum F. J. Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase. J Biol Chem. 1971 Feb 25;246(4):909–916. [PubMed] [Google Scholar]
  7. Chen K. C., Kindt T. J., Krause R. M. Amino-acid sequence of an allotype b4 light chain from a rabbit antibody to streptococcal carbohydrate. Proc Natl Acad Sci U S A. 1974 May;71(5):1995–1998. doi: 10.1073/pnas.71.5.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chen K. C., Kindt T. J., Krause R. M. Primary structure of the L chain from a rabbit homogeneous antibody to streptococcal carbohydrate. II. Sequence determination of peptides from tryptic and peptic digests. J Biol Chem. 1975 May 10;250(9):3289–3296. [PubMed] [Google Scholar]
  9. Chersi A., Alexander C. B., Mage R. Partial primary structure of the immunoglobulin light chain constant region of a single rabbit of b5 allotype. Mol Immunol. 1980 Dec;17(12):1515–1523. doi: 10.1016/0161-5890(80)90177-7. [DOI] [PubMed] [Google Scholar]
  10. Chersi A., Mage R. Allotypic variations in the amino acid sequence of rabbit IgG kappa light chains in the region of tryptophan 146. Mol Immunol. 1980 Jan;17(1):135–138. doi: 10.1016/0161-5890(80)90133-9. [DOI] [PubMed] [Google Scholar]
  11. Dubiski S., Muller P. J. A "new" allotypic specificity (A9) of rabbit immunoglobulin. Nature. 1967 May 13;214(5089):696–697. doi: 10.1038/214696a0. [DOI] [PubMed] [Google Scholar]
  12. Efstratiadis A., Posakony J. W., Maniatis T., Lawn R. M., O'Connell C., Spritz R. A., DeRiel J. K., Forget B. G., Weissman S. M., Slightom J. L. The structure and evolution of the human beta-globin gene family. Cell. 1980 Oct;21(3):653–668. doi: 10.1016/0092-8674(80)90429-8. [DOI] [PubMed] [Google Scholar]
  13. Farnsworth V., Goodfliesh R., Rodkey S., Hood L. Immunoglobulin allotypes of rabbit kappa chains: polymorphism of a control mechanism regulating closely linked duplicated genes? Proc Natl Acad Sci U S A. 1976 Apr;73(4):1293–1296. doi: 10.1073/pnas.73.4.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Greene P. J., Heyneker H. L., Bolivar F., Rodriguez R. L., Betlach M. C., Covarrubias A. A., Backman K., Russel D. J., Tait R., Boyer H. W. A general method for the purification of restriction enzymes. Nucleic Acids Res. 1978 Jul;5(7):2373–2380. doi: 10.1093/nar/5.7.2373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hieter P. A., Max E. E., Seidman J. G., Maizel J. V., Jr, Leder P. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell. 1980 Nov;22(1 Pt 1):197–207. doi: 10.1016/0092-8674(80)90168-3. [DOI] [PubMed] [Google Scholar]
  16. Katz L., Kingsbury D. T., Helinski D. R. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J Bacteriol. 1973 May;114(2):577–591. doi: 10.1128/jb.114.2.577-591.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kindt T. J. Rabbit immunoglobulin allotypes: structure, immunology, and genetics. Adv Immunol. 1975;21:35–86. doi: 10.1016/s0065-2776(08)60218-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Konkel D. A., Maizel J. V., Jr, Leder P. The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes. Cell. 1979 Nov;18(3):865–873. doi: 10.1016/0092-8674(79)90138-7. [DOI] [PubMed] [Google Scholar]
  19. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Milstein C. P., Steinberg A. G., McLaughlin C. L., Solomon A. Amino acid sequence change associated with genetic marker Inv(2) of human immunoglobulin. Nature. 1974 Mar 8;248(5444):160–161. doi: 10.1038/248160a0. [DOI] [PubMed] [Google Scholar]
  22. Milstein C. Variations in amino-acid sequence near the disulphide bridges of Bence-Jones proteins. Nature. 1966 Jan 22;209(5021):370–373. doi: 10.1038/209370a0. [DOI] [PubMed] [Google Scholar]
  23. OUDIN J. Allotypy of rabbit serum proteins. I. Immuno-chemical analysis leading to the individualization of seven main allotypes. J Exp Med. 1960 Jul 1;112:107–124. doi: 10.1084/jem.112.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. OUDIN J. Allotypy of rabbit serum proteins. II. Relationships between various allotypes: their common antigenic specificity, their distribution in a sample population; genetic implications. J Exp Med. 1960 Jul 1;112:125–142. doi: 10.1084/jem.112.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. OUDIN J. L'allotypie de certains antigènes protéidiques du sérum. C R Hebd Seances Acad Sci. 1956 May 23;242(21):2606–2608. [PubMed] [Google Scholar]
  26. OUDIN J. Réaction de précipitation spécifique entre des sérums d'animaux de même espèce. C R Hebd Seances Acad Sci. 1956 May 14;242(20):2489–2490. [PubMed] [Google Scholar]
  27. OUDIN J. [Allotypes of certain serum protein antigens. Immuno-chemical and genetic relationships between 6 principal allotypes observed in rabbit serum]. C R Hebd Seances Acad Sci. 1960 Jan 25;250:770–772. [PubMed] [Google Scholar]
  28. Panet A., van de Sande J. H., Loewen P. C., Khorana H. G., Raae A. J., Lillehaug J. R., Kleppe K. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry. 1973 Dec 4;12(25):5045–5050. doi: 10.1021/bi00749a003. [DOI] [PubMed] [Google Scholar]
  29. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  30. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  31. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  32. Seidman J. G., Leder P. The arrangement and rearrangement of antibody genes. Nature. 1978 Dec 21;276(5690):790–795. doi: 10.1038/276790a0. [DOI] [PubMed] [Google Scholar]
  33. Sikorav J. L., Auffray C., Rougeon F. Structure of the constant and 3' untranslated regions of the murine Balb/c gamma 2a heavy chain messenger RNA. Nucleic Acids Res. 1980 Jul 25;8(14):3143–3155. doi: 10.1093/nar/8.14.3143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Sogn J. A., Kindt T. J. A genetic polymorphism in the constant region of rabbit b4 kappa chains. J Exp Med. 1976 Jun 1;143(6):1475–1482. doi: 10.1084/jem.143.6.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Strosberg A. D., Emorine L., Zeeuws R. The structural polymorphism of the rabbit and other lagomorph allotypes constitute evidence for a control mechanism regulating the expression of closely linked duplicated genes. Ann Immunol (Paris) 1979 Mar-Apr;130(2):157–166. [PubMed] [Google Scholar]
  36. Strosberg A. D., Fraser K. J., Margolies M. N., Haber E. Amino acid sequence of rabbit pneumococcal antibody. I. Light-chain cysteine-containing peptides. Biochemistry. 1972 Dec 19;11(26):4978–4985. doi: 10.1021/bi00776a017. [DOI] [PubMed] [Google Scholar]
  37. Strosberg A. D., Hamers-Casterman C., Van der Loo W., Hamers R. A rabbit with the allotypic phenotype: ala2a3 b4b5b6. J Immunol. 1974 Oct;113(4):1313–1318. [PubMed] [Google Scholar]
  38. Van Hoegaerden M., Strosberg A. D. Sequence of a rabbit anti-Micrococcus lysodeikticus antibody light chain. Biochemistry. 1978 Oct 3;17(20):4311–4317. doi: 10.1021/bi00613a031. [DOI] [PubMed] [Google Scholar]
  39. Yarmush M. L., Sogn J. A., Kindt T. J. Latent allotypes: a window to a genetic enigma. Ann Immunol (Paris) 1979 Mar-Apr;130(2):143–156. [PubMed] [Google Scholar]
  40. van den Berg J., van Ooyen A., Mantei N., Schamböck A., Grosveld G., Flavell R. A., Weissmann C. Comparison of cloned rabbit and mouse beta-globin genes showing strong evolutionary divergence of two homologous pairs of introns. Nature. 1978 Nov 2;276(5683):37–44. doi: 10.1038/276037a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES