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. 1980 May;77(5):2651–2655. doi: 10.1073/pnas.77.5.2651

Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells.

M Jones, R A Bosselman, F A van der Hoorn, A Berns, H Fan, I M Verma
PMCID: PMC349460  PMID: 6248858

Abstract

When uninfected mouse cell DNA is cleaved with restriction endonuclease EcoRI, a DNA fragment of 14.0 kilobases can be identified by hybridization to cloned DNA containing sarcoma specific sequences of Moloney mouse sarcoma virus (M-MSVsrc). The cellular DNA fragment contains the entire M-MSVsrc specific sequences. The 14.0-kilobase EcoRI DNA fragment was cloned in bacteriophage lambda. The sequence organization of a recombinant clone, lambda . MTX-1, was analyzed by restriction endonuclease mapping, nuclease S1 mapping, and electron microscopy. The results indicate that lambda . MTX-1 contains an uninterrupted stretch of 1.0 kilobase similar to that found in the M-MSV genome.

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Selected References

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