Abstract
A human colon carcinoma cell line, HC84S, was established in serum-supplemented medium from a colon tumor line T84 transplanted in nude mice. These cells also grew in a serum-free, synthetic medium supplement with insulin, glucagon, epidermal growth factor, transferrin, hydrocortisone, triiodothyronine, selenium, and ascorbic acid. HC84S cells grew 3 times faster in this medium than in serum-containing medium and formed gland-like structures closely resembling the original tumor morphologically. In serum-containing medium, the cells grew as a monolayer and did not form such structures. Primary cultures from transplantable human colon tumor lines maintained in nude mice and a primary tumor from a patient were established directly in this hormone-supplemented medium in collagen-treated plastic dishes without fibroblast overgrowth. The hormone-supplemented medium may be generally useful for the establishment of human colon carcinoma cell lines.
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- Bottenstein J. E., Sato G. H. Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc Natl Acad Sci U S A. 1979 Jan;76(1):514–517. doi: 10.1073/pnas.76.1.514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bottenstein J., Hayashi I., Hutchings S., Masui H., Mather J., McClure D. B., Ohasa S., Rizzino A., Sato G., Serrero G. The growth of cells in serum-free hormone-supplemented media. Methods Enzymol. 1979;58:94–109. doi: 10.1016/s0076-6879(79)58127-0. [DOI] [PubMed] [Google Scholar]
- Hayashi I., Sato G. H. Replacement of serum by hormones permits growth of cells in a defined medium. Nature. 1976 Jan 15;259(5539):132–134. doi: 10.1038/259132a0. [DOI] [PubMed] [Google Scholar]
- Hutchings S. E., Sato G. H. Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones. Proc Natl Acad Sci U S A. 1978 Feb;75(2):901–904. doi: 10.1073/pnas.75.2.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laburthe M., Rousset M., Boissard C., Chevalier G., Zweibaum A., Rosselin G. Vasoactive intestinal peptide: a potent stimulator of adenosine 3':5'-cyclic monophosphate accumulation in gut carcinoma cell lines in culture. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2772–2775. doi: 10.1073/pnas.75.6.2772. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lichtenberger L., Miller L. R., Erwin D. N., Johnson L. R. Effect of pentagastrin on adult rat duodenal cells in culture. Gastroenterology. 1973 Aug;65(2):242–251. [PubMed] [Google Scholar]
- Mather J. P., Sato G. H. The growth of mouse melanoma cells in hormone-supplemented, serum-free medium. Exp Cell Res. 1979 Apr;120(1):191–200. doi: 10.1016/0014-4827(79)90549-4. [DOI] [PubMed] [Google Scholar]
- McKeehan W. L., Hamilton W. G., Ham R. G. Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2023–2027. doi: 10.1073/pnas.73.6.2023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rizzino A., Sato G. Growth of embryonal carcinoma cells in serum-free medium. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1844–1848. doi: 10.1073/pnas.75.4.1844. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taub M., Chuman L., Saier M. H., Jr, Sato G. Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3338–3342. doi: 10.1073/pnas.76.7.3338. [DOI] [PMC free article] [PubMed] [Google Scholar]