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. 1980 Jun;77(6):3519–3523. doi: 10.1073/pnas.77.6.3519

Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor.

A F Gazdar, W L Chick, H K Oie, H L Sims, D L King, G C Weir, V Lauris
PMCID: PMC349648  PMID: 6106192

Abstract

Continuous cell lines that secrete both insulin and somatostatin were established by two cooperating groups of investiagtors from a serially transplantable, radiation-induced, rat islet cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number, are tumorigenic, and posses amine-handling properties, including high levels of L-dopa decarboxylase and formaldehyde-induced fluorescence. Preliminary analysis of clones revealed a spectrum of insulin secretion from undetectable to relatively high. These clonal cell lines provide important systems to study the biology of insulin and somatostatin.

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Selected References

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