Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Jun;77(6):3625–3629. doi: 10.1073/pnas.77.6.3625

Surface component of primate thymus-derived lymphocytes related to a heavy chain variable region.

J J Marchalonis, G W Warr, J D Rodwell, F Karush
PMCID: PMC349670  PMID: 6774341

Abstract

In a study designed to determine whether T cells of man and higher primates express a surface component related to the variable region of immunoglobulin heavy chain (VH), chickens were immunized with the purified VH fragment of a monoclonal Waldenström macroglobulin. The antibody preparation reacted with a mu chain determinant contained in the Fd fragment and with individual determinants characteristic of the orginal Waldenström protein. As estimated by immunofluorescence analysis, a subpopulation of normal human peripheral T cells (approximately 30%) bound the anti-VH antibody. B-Cell lymphoma lines grown in vitro, as well as some T-cell leukemia lines of the cotton-topped marmoset (Sagiunus oedipus), also bound the anti-VH antibody. The VH-bearing component of the T-cell line 70-N-2 was labeled biosynthetically by incorporation of [3H]leucine and was precipitated specifically by anti-VH antibody. This component was characterized by an apparent mass of 70,000 daltons as assessed by polyacrylamide gel electrophoresis under reducing conditions in buffers containing sodium dodecyl sulfate. These data provide direct support for the hypothesis that some T cells express and synthesize a component related to immunoglobulin heavy chains.

Full text

PDF
3626

Images in this article

3628Image
on p.3628

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binz H., Wigzell H. Antigen-binding, idiotypic T-lymphocyte receptors. Contemp Top Immunobiol. 1977;7:113–177. doi: 10.1007/978-1-4684-3054-7_4. [DOI] [PubMed] [Google Scholar]
  2. Burrows P., LeJeune M., Kearney J. F. Evidence that murine pre-B cells synthesise mu heavy chains but no light chains. Nature. 1979 Aug 30;280(5725):838–840. doi: 10.1038/280838a0. [DOI] [PubMed] [Google Scholar]
  3. Cone R. E., Brown W. C. Isolation of membrane associated immunoglobulins from T lymphocytes by non-ionic detergents. Immunochemistry. 1976 Jul;13(7):571–579. doi: 10.1016/0019-2791(76)90168-3. [DOI] [PubMed] [Google Scholar]
  4. DeLuca D., Warr G. W., Marchalonis J. J. Phylogenetic origins of immune recognition: lymphocyte surface immunoglobulins and antigen binding in the genus Carassius (Teleostii). Eur J Immunol. 1978 Jul;8(7):525–530. doi: 10.1002/eji.1830080713. [DOI] [PubMed] [Google Scholar]
  5. Fechheimer M., Cebra J. J. Isolation and characterization of actin and myosin from B-lymphocytic guinea pig leukemia cells. J Immunol. 1979 Jun;122(6):2590–2597. [PubMed] [Google Scholar]
  6. Fidler I. J., Kripke M. L. Metastasis results from preexisting variant cells within a malignant tumor. Science. 1977 Aug 26;197(4306):893–895. doi: 10.1126/science.887927. [DOI] [PubMed] [Google Scholar]
  7. Haustein D., Marchalonis J. J., Harris A. W. Immunoglobulin of T lymphoma cells. Biosynthesis, surface representation, and partial characterization. Biochemistry. 1975 May 6;14(9):1826–1834. doi: 10.1021/bi00680a004. [DOI] [PubMed] [Google Scholar]
  8. Hämmerling U., Mack C., Pickel H. G. Immunofluorescence analysis of Ig determinants of mouse thymocytes and T cells. Immunochemistry. 1976 Jun;13(6):525–531. doi: 10.1016/0019-2791(76)90329-3. [DOI] [PubMed] [Google Scholar]
  9. Jones V. E., Graves H. E., Orlans E. The detection of F(ab')2-related surface antigens on the thymocytes of children. Immunology. 1976 Feb;30(2):281–288. [PMC free article] [PubMed] [Google Scholar]
  10. Lea T., Førre O. T., Michaelsen T. E., Natvig J. B. Shared idiotypes of human peripheral blood B and T lymphocytes. J Immunol. 1979 Jun;122(6):2413–2417. [PubMed] [Google Scholar]
  11. Lonai P., Ben-Neriah Y., Steinman L., Givol D. Selective participation of immunoglobulin V region and major histocompatibility complex products in antigen binding by T cells. Eur J Immunol. 1978 Dec;8(12):827–832. doi: 10.1002/eji.1830081202. [DOI] [PubMed] [Google Scholar]
  12. Mage R. G. A new look at the biological and genetic significance of rabbit heavy chain allotypes. Ann Immunol (Paris) 1979 Mar-Apr;130(2):105–114. [PubMed] [Google Scholar]
  13. Marchalonis J. J., Bucana C., Hoyer L., Warr G. W., Hanna M. G., Jr Visualization of a guinea pig T lymphocyte surface component cross-reactive with immunoglobulin. Science. 1978 Jan 27;199(4327):433–435. doi: 10.1126/science.74094. [DOI] [PubMed] [Google Scholar]
  14. Marchalonis J. J., Cone R. E., Atwell J. L. Isolation and partial characterization of lymphocyte surface immunoglobulins. J Exp Med. 1972 Apr 1;135(4):956–971. doi: 10.1084/jem.135.4.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marchalonis J. J., Schonfeld S. A. Polypeptide chain structure of sting ray immunoglobulin. Biochim Biophys Acta. 1970 Dec 22;221(3):604–611. doi: 10.1016/0005-2795(70)90232-1. [DOI] [PubMed] [Google Scholar]
  16. McLellan W. L., Ihle J. N. Purification and characterization of a murine tumor cell surface glycoprotein of 75,000 daltons that is related to the major envelope glycoprotein of murine leukemia virus. Virology. 1978 Sep;89(2):547–559. doi: 10.1016/0042-6822(78)90196-4. [DOI] [PubMed] [Google Scholar]
  17. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  18. Moroz C., Hahn Y. Cell-surface immunoglobulin human thymus cells and its biosynthesis in vitro. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3716–3720. doi: 10.1073/pnas.70.12.3716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moseley J. M., Marchalonis J. J., Harris A. W., Pye J. Molecular properties of T lymphoma immunoglobulin. I. Serological and general physicochemical properties. J Immunogenet. 1977 Aug;4(4):233–248. doi: 10.1111/j.1744-313x.1977.tb00906.x. [DOI] [PubMed] [Google Scholar]
  20. Neubauer R. H., Rabin H., Hopkins R. F., 3rd, Levy B. M. Characteristics of cell lines established from Epstein-Barr virus induced marmoset tumors. Primates Med. 1978;10:156–162. [PubMed] [Google Scholar]
  21. Rajewsky K., Eichmann K. Antigen receptors of T helper cells. Contemp Top Immunobiol. 1977;7:69–112. doi: 10.1007/978-1-4684-3054-7_3. [DOI] [PubMed] [Google Scholar]
  22. Rodwell J. D., Karush F. A general method for the isolation of the VH domain from IgM and other immunoglobulins. J Immunol. 1978 Oct;121(4):1528–1531. [PubMed] [Google Scholar]
  23. Strnad B. C., Neubauer R. H., Rabin H. Biochemical identification of primate lymphoid cell-surface glycoproteins. Int J Cancer. 1979 Jan 15;23(1):76–81. doi: 10.1002/ijc.2910230114. [DOI] [PubMed] [Google Scholar]
  24. Szenberg A., Marchalonis J. J., Warner N. L. Direct demonstration of murine thymus-dependent cell surface endogenous immunoglobin. Proc Natl Acad Sci U S A. 1977 May;74(5):2113–2117. doi: 10.1073/pnas.74.5.2113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wang A. C., Fudenberg H. H. Genetic control of gamma chain synthesis: a chemical and evolutionary study of the Gm(a)factor of immunoglobulins. J Mol Biol. 1969 Sep 28;44(3):493–500. doi: 10.1016/0022-2836(69)90375-1. [DOI] [PubMed] [Google Scholar]
  26. Warner N. L. Membrane immunoglobulins and antigen receptors on B and T lymphocytes. Adv Immunol. 1974;19(0):67–216. doi: 10.1016/s0065-2776(08)60252-7. [DOI] [PubMed] [Google Scholar]
  27. Warr G. W., Lee J. C., Marchalonis J. J. Null cells in the mouse possess membrane immunoglobulins. J Immunol. 1978 Nov;121(5):1767–1772. [PubMed] [Google Scholar]
  28. Warr G. W., Marton G., Szenberg A., Marchalonis J. J. Reactions of chicken antibodies with immunoglobulins of mouse serum and T cells. Immunochemistry. 1978 Sep;15(9):615–622. doi: 10.1016/0161-5890(78)90033-0. [DOI] [PubMed] [Google Scholar]
  29. Whiteside T. L., Rabin B. S. Surface immunoglobulin on activated human peripheral blood thymus-derived cells. J Clin Invest. 1976 Mar;57(3):762–771. doi: 10.1172/JCI108335. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES