Abstract
A variant of apolipoprotein B has been observed in the lymph lipoproteins [chylomicrons, very low density lipoproteins (VLDL), and low density lipoproteins (LDL)] of rats, in the plasma VLDL of fed rats, and in the plasma VLDL and LDL of rats fed a high-fat, high-cholesterol diet. It is the sole apolipoprotein B in the chylomicrons and VLDL of lymph. It differs from the apolipoprotein B of normal plasma LDL in its immunological properties and in its apparent molecular weight from electrophoresis on 3.5% NaDodSO4/polyacrylamide gel.
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- Cham B. E., Knowles B. R. A solvent system for delipidation of plasma or serum without protein precipitation. J Lipid Res. 1976 Mar;17(2):176–181. [PubMed] [Google Scholar]
- Eaton R. P., Kipnis D. M. Radioimmunoassay of beta lipoprotein-protein of rat serum. J Clin Invest. 1969 Aug;48(8):1387–1396. doi: 10.1172/JCI106104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eisenberg S., Levy R. I. Lipoprotein metabolism. Adv Lipid Res. 1975;13:1–89. [PubMed] [Google Scholar]
- Faergeman O., Sata T., Kane J. P., Havel R. J. Metabolism of apoprotein B of plasma very low density lipoproteins in the rat. J Clin Invest. 1975 Dec;56(6):1396–1403. doi: 10.1172/JCI108220. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fidge N. H., Poulis P. Metabolic heterogeneity in the formation of low density lipoprotein from very low density lipoprotein in the rat: evidence for the independent production of a low density lipoprotein subfraction. J Lipid Res. 1978 Mar;19(3):342–349. [PubMed] [Google Scholar]
- Fisher W. R., Hammond M. G., Warmke G. L. Measurements of the molecular weight variability of plasma low density lipoproteins among normals and subjects with hyper- -lipoproteinemia. Demonstration of macromolecular heterogeneity. Biochemistry. 1972 Feb 15;11(4):519–525. doi: 10.1021/bi00754a006. [DOI] [PubMed] [Google Scholar]
- Fless G. M., Scanu A. M. Isolation and characterization of the three major low density lipoproteins from normolipidemic rhesus monkeys (Macaca mulatta). J Biol Chem. 1979 Sep 10;254(17):8653–8661. [PubMed] [Google Scholar]
- Glickman R. M., Green P. H. The intestine as a source of apolipoprotein A1. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2569–2573. doi: 10.1073/pnas.74.6.2569. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glickman R. M., Khorana J., Kilgore A. Localization of apolipoprotein B in intestinal epithelial cells. Science. 1976 Sep 24;193(4259):1254–1255. doi: 10.1126/science.183265. [DOI] [PubMed] [Google Scholar]
- Green P. H., Glickman R. M., Saudek C. D., Blum C. B., Tall A. R. Human intestinal lipoproteins. Studies in chyluric subjects. J Clin Invest. 1979 Jul;64(1):233–242. doi: 10.1172/JCI109444. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hay R., Getz G. S. Translation in vivo and in vitro of proteins resembling apoproteins of rat plasma very low density lipoprotein. J Lipid Res. 1979 Mar;20(3):334–348. [PubMed] [Google Scholar]
- Imaizumi K., Havel R. J., Fainaru M., Vigne J. L. Origin and transport of the A-I and arginine-rich apolipoproteins in mesenteric lymph of rats. J Lipid Res. 1978 Nov;19(8):1038–1046. [PubMed] [Google Scholar]
- Jackson R. L., Morrisett J. D., Gotto A. M., Jr Lipoprotein structure and metabolism. Physiol Rev. 1976 Apr;56(2):259–316. doi: 10.1152/physrev.1976.56.2.259. [DOI] [PubMed] [Google Scholar]
- Kane J. P. A rapid electrophoretic technique for identification of subunit species of apoproteins in serum lipoproteins. Anal Biochem. 1973 Jun;53(2):350–364. doi: 10.1016/0003-2697(73)90081-x. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lusk L. T., Walker L. F., DuBien L. H., Getz G. S. Isolation and partial characterization of high-density lipoprotein HDL1 from rat plasma by gradient centrifugation. Biochem J. 1979 Oct 1;183(1):83–90. doi: 10.1042/bj1830083. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osborne J. C., Jr, Brewer H. B., Jr The plasma lipoproteins. Adv Protein Chem. 1977;31:253–337. doi: 10.1016/s0065-3233(08)60220-x. [DOI] [PubMed] [Google Scholar]
- Pottenger L. A., Getz G. S. Serum lipoprotein accumulation in the livers of orotic acid-fed rats. J Lipid Res. 1971 Jul;12(4):450–459. [PubMed] [Google Scholar]
- Reardon M. F., Fidge N. H., Nestel P. J. Catabolism of very low density lipoprotein B apoprotein in man. J Clin Invest. 1978 Mar;61(3):850–860. doi: 10.1172/JCI108999. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rudel L. L., Pitts L. L., 2nd, Nelson C. A. Characterization of plasma low density lipoproteins on nonhuman primates fed dietary cholesterol. J Lipid Res. 1977 Mar;18(2):211–222. [PubMed] [Google Scholar]
- Scanu A., Pollard H., Hirz R., Kothary K. On the conformational instability of human serum low-density lipoprotein: effect of temperature. Proc Natl Acad Sci U S A. 1969 Jan;62(1):171–178. doi: 10.1073/pnas.62.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaefer E. J., Eisenberg S., Levy R. I. Lipoprotein apoprotein metabolism. J Lipid Res. 1978 Aug;19(6):667–687. [PubMed] [Google Scholar]
- Schonfeld G., Bell E., Alpers D. H. Intestinal apoproteins during fat absorption. J Clin Invest. 1978 Jun;61(6):1539–1550. doi: 10.1172/JCI109074. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith L. C., Pownall H. J., Gotto A. M., Jr The plasma lipoproteins: structure and metabolism. Annu Rev Biochem. 1978;47:751–757. doi: 10.1146/annurev.bi.47.070178.003535. [DOI] [PubMed] [Google Scholar]
- Stephens R. E. High-resolution preparative SDS-polyacrylamide gel electrophoresis: fluorescent visualization and electrophoretic elution-concentration of protein bands. Anal Biochem. 1975 May 12;65(1-2):369–379. doi: 10.1016/0003-2697(75)90521-7. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
- Windmueller H. G., Herbert P. N., Levy R. I. Biosynthesis of lymph and plasma lipoprotein apoproteins by isolated perfused rat liver and intestine. J Lipid Res. 1973 Mar;14(2):215–223. [PubMed] [Google Scholar]
- Windmueller H. G., Lindgren F. T., Lossow W. J., Levy R. I. On the nature of circulating lipoproteins of intestinal origin in the rat. Biochim Biophys Acta. 1970 May 5;202(3):507–516. doi: 10.1016/0005-2760(70)90121-9. [DOI] [PubMed] [Google Scholar]
- Windmueller H. G., Spaeth A. E. Fat transport and lymph and plasma lipoprotein biosynthesis by isolated intestine. J Lipid Res. 1972 Jan;13(1):92–105. [PubMed] [Google Scholar]
- Witztum J. L., Schonfeld G. Carbohydrate diet-induced changes in very low density lipoprotein composition and structure. Diabetes. 1978 Dec;27(12):1215–1229. doi: 10.2337/diab.27.12.1215. [DOI] [PubMed] [Google Scholar]
- Wu A. L., Windmueller H. G. Identification of circulating apolipoproteins synthesized by rat small intestine in vivo. J Biol Chem. 1978 Apr 25;253(8):2525–2528. [PubMed] [Google Scholar]