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. 1980 Aug;77(8):4578–4582. doi: 10.1073/pnas.77.8.4578

Hormonal and immunological aspects of the phylogeny of sex steroid binding plasma protein.

J M Renoir, C Mercier-Bodard, E E Baulieu
PMCID: PMC349887  PMID: 6933505

Abstract

Sex steoid binding plasma protein (Sbp) in man and in monkeys binds the androgens dihydrotestosterone and testosterone and the estrogen estradiol with high affinity (Kd approximately 0.5, 1, and 2 nM, respectively). Detailed studies of steroid binding specificity give the same results in all primates, except that in humans and chimpanzees estrone does not compete for dihydrotestosterone binding. In other mammals, Sbps of Artiodactyla and Lagomorpha have the same range of affinities for androgens but they do not bind estradiol to any significant extent (Kd > 280 nM). The dog has an unusual Sbp (Kd for dihydrotestosterone, 7.1 nM; for estradiol, 125 nM), and rodents do not have a specific dihydrotestosterone-binding plasma protein. Gel filtration and immunoelectrophoretic experiments have been performed with a monospecific antiserum against human Sbp. The results indicate variable crossreactivities with Sbps of primates (from complete in chimpanzee and gorilla to weak in Prosimii). No crossreaction was observed with specific androgen-binding plasma proteins of other species. These results suggest the evolutionary emergence of bifunctional Sbp.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bordin S., Lewis J., Petra P. H. Monospecific antibodies to the sex steroid-binding protein (SBP) of human and rabbit sera: cross-reactivity with other species. Biochem Biophys Res Commun. 1978 Nov 14;85(1):391–401. doi: 10.1016/s0006-291x(78)80055-2. [DOI] [PubMed] [Google Scholar]
  2. Burke C. W., Anderson D. C. Sex-hormone-binding globulin is an oestrogen amplifier. Nature. 1972 Nov 3;240(5375):38–40. doi: 10.1038/240038a0. [DOI] [PubMed] [Google Scholar]
  3. Corvol P. L., Chrambach A., Rodbard D., Bardin C. W. Physical properties and binding capacity of testosterone-estradiol-binding globulin in human plasma, determined by polyacrylamide gel electrophoresis. J Biol Chem. 1971 Jun 10;246(11):3435–3443. [PubMed] [Google Scholar]
  4. Corvol P., Bardin C. W. Species distribution of testosterone-binding globulin. Biol Reprod. 1973 Apr;8(3):277–282. doi: 10.1093/biolreprod/8.3.277. [DOI] [PubMed] [Google Scholar]
  5. Fostier A., Breton B. Binding of steroids by plasma of a teleost: the rainbow trout, Salmo gairdnerii. J Steroid Biochem. 1975 Mar-Apr;6(3-4):345–351. doi: 10.1016/0022-4731(75)90154-5. [DOI] [PubMed] [Google Scholar]
  6. Freeman H. C., Idler D. R. Sex hormone binding proteins. II. Isolation from serum of an elasmobranch (Raja radiata). Gen Comp Endocrinol. 1969 Aug;13(1):83–91. doi: 10.1016/0016-6480(69)90224-x. [DOI] [PubMed] [Google Scholar]
  7. Gauthier-Wright F., Baudot N., Mauvais-Jarvis P. Testosterone-binding globulin in stump-tailed macaque monkeys (Macaca speciosa). Endocrinology. 1973 Dec;93(6):1277–1286. doi: 10.1210/endo-93-6-1277. [DOI] [PubMed] [Google Scholar]
  8. Hansson V., Ritzén E. M., French F. S., Weddington S. C., Nayfeh S. N., Reusch E., Attramadal A. Demonstration of a specific binding protein for testosterone and 5alpha-dihydrotestosterone in rabbit serum. Separation from the corticosteroid binding globulin (CBG). Steroids. 1973 Aug;22(2):185–192. doi: 10.1016/0039-128x(73)90084-6. [DOI] [PubMed] [Google Scholar]
  9. Mahoudeau J. A., Corvol P. Rabbit testosterone-binding-globulin. I. Physico-chemical properties. Endocrinology. 1973 Apr;92(4):1113–1119. doi: 10.1210/endo-92-4-1113. [DOI] [PubMed] [Google Scholar]
  10. Martin B., Ozon R. Steroid-protein interactions in nonmammalian vertebrates. II. Steroids binding proteins in the serum of amphibians; a physiological approach. Biol Reprod. 1975 Nov;13(4):371–380. doi: 10.1095/biolreprod13.4.371. [DOI] [PubMed] [Google Scholar]
  11. Mercier-Bodard C., Alfsen A., Baulieu E. E. Sex steroid binding plasma protein (SBP). Acta Endocrinol Suppl (Copenh) 1970;147:204–224. doi: 10.1530/acta.0.065s204. [DOI] [PubMed] [Google Scholar]
  12. Mercier-Bodard C., Baulieu E. E. Affinité pour l'oestradiol de la protéine plasmatique de liaison de la testostérone. C R Acad Sci Hebd Seances Acad Sci D. 1968 Aug 12;267(7):804–807. [PubMed] [Google Scholar]
  13. Mercier-Bodard C., Renoir J. M., Baulieu E. E. Further characterization and immunological studies of human sex steroid binding plasma protein. J Steroid Biochem. 1979 Jul;11(1A):253–259. doi: 10.1016/0022-4731(79)90305-4. [DOI] [PubMed] [Google Scholar]
  14. Mickelson K. E., Pétra P. H. Purification and characterization of the sex steroid-binding protein of rabbit serum. Comparison with the human protein. J Biol Chem. 1978 Aug 10;253(15):5293–5298. [PubMed] [Google Scholar]
  15. Mickelson K. E., Pétra P. H. Purification of the sex steroid binding protein from human serum. Biochemistry. 1975 Mar 11;14(5):957–963. doi: 10.1021/bi00676a013. [DOI] [PubMed] [Google Scholar]
  16. Murphy B. E. Further studies of the specificity of the sex hormone-binding globulin of human plasma. Steroids. 1970 Dec;16(6):791–798. doi: 10.1016/s0039-128x(70)80155-6. [DOI] [PubMed] [Google Scholar]
  17. Nunez E., Savu L., Engelmann F., Benassayag C., Crépy O., Jayle M. F. Origine embryonnaire de la protéine sérique fixant l'oestrone et l'oestradiol chez la ratte impubère. C R Acad Sci Hebd Seances Acad Sci D. 1971 Jul 12;273(2):242–245. [PubMed] [Google Scholar]
  18. Pétra P. H., Schiller H. S. Sex steroid binding protein in the plasma of Macaca nemestrina. J Steroid Biochem. 1977 Jun;8(6):655–661. doi: 10.1016/0022-4731(77)90293-x. [DOI] [PubMed] [Google Scholar]
  19. Radanyi C., Redeuilh G., Eigenmann E., Lebeau M. C., Massol N., Secco C., Baulieu E. E., Richard-Foy H. Production et détection d'anticorps antirécepteur de l'oesstradiol d'utérus de veau. Interaction avec le récepteur d'oviducte de poule. C R Seances Acad Sci D. 1979 Jan 15;288(2):255–258. [PubMed] [Google Scholar]
  20. Renoir J. M., Fox L. L., Baulieu E. E., Mercier-Bodard C. An antiserum specific for human sex steroid-binding plasma protein (SBP). FEBS Lett. 1977 Mar 15;75(1):83–88. doi: 10.1016/0014-5793(77)80058-6. [DOI] [PubMed] [Google Scholar]
  21. Rosenbaum W., Christy N. P., Kelly W. G. Electrophoretic evidence for the presence of an estrogen-binding beta-globulin in human plasma. J Clin Endocrinol Metab. 1966 Dec;26(12):1399–1403. doi: 10.1210/jcem-26-12-1399. [DOI] [PubMed] [Google Scholar]
  22. Rosenthal H. E. A graphic method for the determination and presentation of binding parameters in a complex system. Anal Biochem. 1967 Sep;20(3):525–532. doi: 10.1016/0003-2697(67)90297-7. [DOI] [PubMed] [Google Scholar]
  23. Rosner W., Christy N. P., Kelly W. G. Partial purification and preliminary characterization of estrogen-binding globulins from human plasma. Biochemistry. 1969 Jul;8(7):3100–3108. doi: 10.1021/bi00835a061. [DOI] [PubMed] [Google Scholar]
  24. Rosner W., Darmstadt R. A. Demonstration and partial characterization of a rabbit serum protein which binds testosterone and dihydrotestosterone. Endocrinology. 1973 Jun;92(6):1700–1707. doi: 10.1210/endo-92-6-1700. [DOI] [PubMed] [Google Scholar]
  25. Rosner W., Smith R. N. Isolation and characterization of the testosterone-estradiol-binding globulin from human plasma. Use of a novel affinity column. Biochemistry. 1975 Nov 4;14(22):4813–4820. doi: 10.1021/bi00693a006. [DOI] [PubMed] [Google Scholar]
  26. Suzuki Y., Itagaki E., Mori H., Hosoya T. Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization. J Biochem. 1977 Jun;81(6):1721–1731. doi: 10.1093/oxfordjournals.jbchem.a131632. [DOI] [PubMed] [Google Scholar]
  27. Suzuki Y., Okumura Y., Sinohara H. Purification and characterization of testosterone-binding globulin of canine serum. J Biochem. 1979 May;85(5):1195–1203. [PubMed] [Google Scholar]
  28. Tabei T., Mickelson K. E., Neuhaus S., Petra P. H. Sex steroid binding protein (SBP) in dog plasma. J Steroid Biochem. 1978 Oct;9(10):983–988. doi: 10.1016/0022-4731(78)90062-6. [DOI] [PubMed] [Google Scholar]
  29. Uriel J., de Nechaud B., Dupiers M. Estrogen-binding properties of rat, mouse and man fetospecific serum proteins. Demonstration by immuno-autoradiographic methods. Biochem Biophys Res Commun. 1972 Feb 16;46(3):1175–1180. doi: 10.1016/s0006-291x(72)80098-6. [DOI] [PubMed] [Google Scholar]
  30. Van Baelen H., Heyns W., Schonne E., de Moor P. An estradiol binding globulin in human serum: partial purification. Ann Endocrinol (Paris) 1968;29(Suppl):153–158. [PubMed] [Google Scholar]
  31. Wenn R. V., Kamberi I. A., Keyvanjah M., Johannes A. Distribution of testosterone-estradiol binding globulin (TeBG) in the higher vertebrates. Endokrinologie. 1977 Jul;69(2):151–156. [PubMed] [Google Scholar]

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