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. 1980 Nov;77(11):6521–6525. doi: 10.1073/pnas.77.11.6521

Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.

S L Zipursky, K J Marians
PMCID: PMC350317  PMID: 6109282

Abstract

The Escherichia coli replication factor Y has been characterized as a phi X174 (+) strand specific DNA-dependent phosphohydrolase. In conjunction with other E. coli replication proteins, factor Y is involved in the formation of heterogeneous primers that are elongated by the E. coli DNA polymerase III elongation machinery. We report here that the heat-denatured DNAs of plasmids pBR322 and ColE1 serve as effectors for the hydrolysis of ATP by factor Y. The DNA sequences of pBR322 responsible for factor Y effector activity have been localized. Two separate regions of the pBR322 chromosome support Y ATPase activity. These sequences are near the replication origin and are located on opposite DNA strands.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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