Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Apr;32(1):137–144. doi: 10.1128/iai.32.1.137-144.1981

Protein synthesis in HeLa or Henle 407 cells infected with Shigella dysenteriae 1, Shigella flexneri 2a, or Salmonella typhimurium W118.

T L Hale, S B Formal
PMCID: PMC350598  PMID: 7012026

Abstract

The incorporation of [14C]leucine into protein was studied in two mammalian cell lines which had been infected with strains of Shigella dysenteriae 1, Shigella flexneri 2a, or Salmonella typhimurium W118. These cell lines differed in susceptibility to the effects of exogenously applied Shiga cytotoxin. All invasive shigella strains (which synthesize this toxin to a greater or lesser degree) were found to inhibit protein synthesis in both cell lines with equal efficiency. Leucine accumulation continued in these cells, but the labeled amino acid was preferentially incorporated into bacterial protein. S. typhimurium W118, which has not been shown to elaborate a Shiga-like toxin, had little effect on protein synthesis in infected host cells.

Full text

PDF
140

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRANHAM S. E., DACK G. M., RIGGS D. B. Studies with Shigella dysenteriae (Shiga). IV. Immunological reactions in monkeys to the toxins in isolated intestinal pouches. J Immunol. 1953 Jan;70(1):103–113. [PubMed] [Google Scholar]
  2. BRIDGWATER F. A., MORGAN R. S., ROWSON K. E., WRIGHT G. P. The neurotoxin of Shigella shigae: morphological and functional lesions produced in the central nervous system of rabbits. Br J Exp Pathol. 1955 Oct;36(5):447–453. [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  4. Brown J. E., Rothman S. W., Doctor B. P. Inhibition of protein synthesis in intact HeLa cells by Shigella dysenteriae 1 toxin. Infect Immun. 1980 Jul;29(1):98–107. doi: 10.1128/iai.29.1.98-107.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown J. E., Ussery M. A., Leppla S. H., Rothman S. W. Inhibition of protein synthesis by Shiga toxin: activation of the toxin and inhibition of peptide elongation. FEBS Lett. 1980 Aug 11;117(1):84–88. doi: 10.1016/0014-5793(80)80918-5. [DOI] [PubMed] [Google Scholar]
  6. Donowitz M., Binder H. J. Effect of enterotoxins of Vibrio cholerae, Escherichia coli, and Shigella dysenteriae type 1 on fluid and electrolyte transport in the colon. J Infect Dis. 1976 Aug;134(2):135–143. doi: 10.1093/infdis/134.2.135. [DOI] [PubMed] [Google Scholar]
  7. Gartler S. M. Apparent Hela cell contamination of human heteroploid cell lines. Nature. 1968 Feb 24;217(5130):750–751. doi: 10.1038/217750a0. [DOI] [PubMed] [Google Scholar]
  8. Gemski P., Jr, Takeuchi A., Washington O., Formal S. B. Shigellosis due to Shigella dysenteriae. 1. Relative importance of mucosal invasion versus toxin production in pathogenesis. J Infect Dis. 1972 Nov;126(5):523–530. doi: 10.1093/infdis/126.5.523. [DOI] [PubMed] [Google Scholar]
  9. Gentry M. K., Dalrymple J. M. Quantitative microtiter cytotoxicity assay for Shigella toxin. J Clin Microbiol. 1980 Sep;12(3):361–366. doi: 10.1128/jcm.12.3.361-366.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hale T. L., Bonventre P. F. Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infect Immun. 1979 Jun;24(3):879–886. doi: 10.1128/iai.24.3.879-886.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hale T. L., Formal S. B. Cytotoxicity of Shigella dysenteriae 1 for cultured mammalian cells. Am J Clin Nutr. 1980 Nov;33(11 Suppl):2485–2490. doi: 10.1093/ajcn/33.11.2485. [DOI] [PubMed] [Google Scholar]
  12. Kent T. H., Formal S. B., Labrec E. H. Salmonella gastroenteritis in rhesus monkeys. Arch Pathol. 1966 Sep;82(3):272–279. [PubMed] [Google Scholar]
  13. Keusch G. T. Ecological control of the bacterial diarrheas: a scientific strategy. Am J Clin Nutr. 1978 Dec;31(12):2208–2218. doi: 10.1093/ajcn/31.12.2208. [DOI] [PubMed] [Google Scholar]
  14. Keusch G. T., Grady G. F., Mata L. J., McIver J. The pathogenesis of Shigella diarrhea. I. Enterotoxin production by Shigella dysenteriae I. J Clin Invest. 1972 May;51(5):1212–1218. doi: 10.1172/JCI106915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Keusch G. T., Grady G. F., Takeuchi A., Sprinz H. The pathogenesis of shigella diarrhea. II. Enterotoxin-induced acute enteritis in the rabbit ileum. J Infect Dis. 1972 Jul;126(1):92–95. doi: 10.1093/infdis/126.1.92. [DOI] [PubMed] [Google Scholar]
  16. Keusch G. T., Jacewicz M., Levine M. M., Hornick R. B., Kochwa S. Pathogenesis of shigella diarrhea. Serum anticytotoxin antibody response produced by toxigenic and nontoxigenic Shigella dysenteriae 1. J Clin Invest. 1976 Jan;57(1):194–202. doi: 10.1172/JCI108259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Keusch G. T., Jacewicz M. Pathogenesis of Shigella diarrhea. VII. Evidence for a cell membrane toxin receptor involving beta1 leads to 4-linked N-acetyl-D-glucosamine oligomers. J Exp Med. 1977 Aug 1;146(2):535–546. doi: 10.1084/jem.146.2.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Keusch G. T., Jacewicz M. The pathogenesis of Shigella diarrhea. V. Relationship of shiga enterotoxin, neurotoxin, and cytotoxin. J Infect Dis. 1975 May;131 (Suppl):S33–S39. doi: 10.1093/infdis/131.supplement.s33. [DOI] [PubMed] [Google Scholar]
  19. Keusch G. T., Jacewicz M. The pathogenesis of Shigella diarrhea. VI. Toxin and antitoxin in Shigella flexneri and Shigella sonnei infections in humans. J Infect Dis. 1977 Apr;135(4):552–556. doi: 10.1093/infdis/135.4.552. [DOI] [PubMed] [Google Scholar]
  20. Keusch G. T. Pathogenesis of Shigella diarrhea. 3. Effects of shigella enterotoxin in cell culture. Trans N Y Acad Sci. 1973 Jan;35(1):51–58. doi: 10.1111/j.2164-0947.1973.tb01503.x. [DOI] [PubMed] [Google Scholar]
  21. Labrec E. H., Schneider H., Magnani T. J., Formal S. B. EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY. J Bacteriol. 1964 Nov;88(5):1503–1518. doi: 10.1128/jb.88.5.1503-1518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Levine M. M., DuPont H. L., Formal S. B., Hornick R. B., Takeuchi A., Gangarosa E. J., Snyder M. J., Libonati J. P. Pathogenesis of Shigella dysenteriae 1 (Shiga) dysentery. J Infect Dis. 1973 Mar;127(3):261–270. doi: 10.1093/infdis/127.3.261. [DOI] [PubMed] [Google Scholar]
  23. O'Brien A. D., Thompson M. R., Gemski P., Doctor B. P., Formal S. B. Biological properties of Shigella flexneri 2A toxin and its serological relationship to Shigella dysenteriae 1 toxin. Infect Immun. 1977 Mar;15(3):796–798. doi: 10.1128/iai.15.3.796-798.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
  25. Olenick J. G., Wolfe A. D. Shigella toxin inhibition of binding and translation of polyuridylic acid by Escherichia coli ribosomes. J Bacteriol. 1980 Mar;141(3):1246–1250. doi: 10.1128/jb.141.3.1246-1250.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Olsnes S., Eiklid K. Isolation and characterization of Shigella shigae cytotoxin. J Biol Chem. 1980 Jan 10;255(1):284–289. [PubMed] [Google Scholar]
  27. Takeuchi A., Formal S. B., Sprinz H. Exerimental acute colitis in the Rhesus monkey following peroral infection with Shigella flexneri. An electron microscope study. Am J Pathol. 1968 Mar;52(3):503–529. [PMC free article] [PubMed] [Google Scholar]
  28. Takeuchi A., Sprinz H. Electron-Microscope Studies of Experimental Salmonella Infection in the Preconditioned Guinea Pig: II. Response of the Intestinal Mucosa to the Invasion by Salmonella typhimurium. Am J Pathol. 1967 Jul;51(1):137–161. [PMC free article] [PubMed] [Google Scholar]
  29. Thompson M. R., Steinberg M. S., Gemski P., Formal S. B., Doctor B. P. Inhibition of in vitro protein synthesis by Shigella dysenteriae 1 toxin. Biochem Biophys Res Commun. 1976 Aug 9;71(3):783–788. doi: 10.1016/0006-291x(76)90899-8. [DOI] [PubMed] [Google Scholar]
  30. VICARI G., OLITZKI A. L., OLITZKI Z. The action of the thermolabile toxin of Shigella dysenteriae on cells cultivated in vitro. Br J Exp Pathol. 1960 Apr;41:179–189. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES