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. 1981 Aug;33(2):546–554. doi: 10.1128/iai.33.2.546-554.1981

Isolation of the intracellular stage of Trypanosoma cruzi and its interaction with mouse macrophages in vitro.

R M Carvalho, M N Meirelles, W de Souza, W Leon
PMCID: PMC350735  PMID: 7024133

Abstract

Interaction between Trypanosoma cruzi spheromastigotes (amastigotes) and mouse macrophages was studied. The spheromastigotes, isolated from the spleens of infected mice, were incorporated and digested by the macrophages. By use of horseradish peroxidase labeling of the macrophage lysosomes we showed that fusion of lysosomes with phagocytic vacuoles containing T. cruzi occurred. Parasites showing alterations indicative of digestion were seen inside the phagocytic vacuoles. Our results suggest that intracellular sphermastigotes of T. cruzi, isolated from the spleens of infected mice, and not able to induce a protective infection in mouse macrophages maintained in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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