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. 1982 Mar;35(3):874–879. doi: 10.1128/iai.35.3.874-879.1982

Enhanced Plasmodium falciparum merozoite phagocytosis by monocytes from immune individuals.

S Khusmith, P Druilhe, M Gentilini
PMCID: PMC351128  PMID: 7040248

Abstract

Phagocytosis of merozoites and schizont-infected erythrocytes prepared from continuous cultures by peripheral blood monocytes from patients with falciparum malaria was investigated with an in vitro assay. Monocytes do ingest merozoites of Plasmodium falciparum, but rarely phagocytose parasitized or nonparasitized erythrocytes in the absence of immune serum. The monocytes from hyperimmune subjects were significantly more efficient in the ingestion of merozoites than were those obtained from sensitized or noninfected subjects. These data indicate, first, that the merozoite rather than the parasitized erythrocyte is the specific target for blood phagocytic cells in human falciparum malaria and, second, that the phagocytosis of merozoites by peripheral blood monocytes increases depending on the level of specific immunity.

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Selected References

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  1. Brooks C., Kreier J. P. Role of the surface coat in in vitro attachment and phagocytosis of Plasmodium berghei by peritoneal macrophages. Infect Immun. 1978 Jun;20(3):827–835. doi: 10.1128/iai.20.3.827-835.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown I. N. Immunological aspects of malaria infection. Adv Immunol. 1969;11:267–349. doi: 10.1016/s0065-2776(08)60481-2. [DOI] [PubMed] [Google Scholar]
  3. Brown K. N., Brown I. N., Trigg P. I., Phillips R. S., Hills L. A. Immunity to malaria. II. Serological response of monkeys sensitized by drug-suppressed infection or by dead parasitized cells in Freund's complete adjuvant. Exp Parasitol. 1970 Oct;28(2):318–338. doi: 10.1016/0014-4894(70)90102-5. [DOI] [PubMed] [Google Scholar]
  4. Brown K. N., Hills L. A. Macrophage "activation" and the action of opsonizing antibodies in malaria. Trans R Soc Trop Med Hyg. 1971;65(1):6–6. [PubMed] [Google Scholar]
  5. COHEN S., McGREGOR I. A., CARRINGTON S. Gamma-globulin and acquired immunity to human malaria. Nature. 1961 Nov 25;192:733–737. doi: 10.1038/192733a0. [DOI] [PubMed] [Google Scholar]
  6. Cantrell W., Elko E. E. Plasmodium berghei: phagocytic activtiy in two strains of rats. Exp Parasitol. 1976 Oct;40(2):281–285. doi: 10.1016/0014-4894(76)90092-8. [DOI] [PubMed] [Google Scholar]
  7. Chow J. S., Kreier J. P. Plasmodium berghei: adherence and phagocytosis by rat macrophages in vitro. Exp Parasitol. 1972 Feb;31(1):13–18. doi: 10.1016/0014-4894(72)90042-2. [DOI] [PubMed] [Google Scholar]
  8. Criswell B. S., Butler W. T., Rossen R. D., Knight V. Murine malaria: the role of humoral factors and macrophages in destruction of parasitized erythrocytes. J Immunol. 1971 Jul;107(1):212–221. [PubMed] [Google Scholar]
  9. Druilhe P., Jacquier P., Lambert D., Gentilini M. Plasmodium falciparum in vitro culture: improvements using umbilical cord serum and medium modifications. Tropenmed Parasitol. 1980 Dec;31(4):409–413. [PubMed] [Google Scholar]
  10. Kumagai K., Itoh K., Hinuma S., Tada M. Pretreatment of plastic Petri dishes with fetal calf serum. A simple method for macrophage isolation. J Immunol Methods. 1979;29(1):17–25. doi: 10.1016/0022-1759(79)90121-2. [DOI] [PubMed] [Google Scholar]
  11. Lustig H. J., Nussenzweig V., Nussenzweig R. S. Erythrocyte membrane-associated immunoglobulins during malaria infection of mice. J Immunol. 1977 Jul;119(1):210–216. [PubMed] [Google Scholar]
  12. MacCallum D. K. A study of macrophage--pulmonary vascular bed interactions in malaria-infected hamsters. J Reticuloendothel Soc. 1969 Jun;6(3):253–270. [PubMed] [Google Scholar]
  13. MacCallum D. K. Time sequence study on the hepatic system of macrophages in malaria-infected hamsters. J Reticuloendothel Soc. 1969 Jun;6(3):232–252. [PubMed] [Google Scholar]
  14. Mogensen C. E. The glomerular permeability determined by dextran clearance using Sephadex gel filtration. Scand J Clin Lab Invest. 1968;21(1):77–82. doi: 10.3109/00365516809076979. [DOI] [PubMed] [Google Scholar]
  15. Phillips R. S., Wolstencroft R. A., Brown I. N., Brown K. N., Dumonde D. C. Immunity to malaria. 3. Possible occurrence of a cell-mediated immunity to Plasmodium knowlesi in chronically infected and Freund's complete adjuvant-sensitized monkeys. Exp Parasitol. 1970 Oct;28(2):339–355. doi: 10.1016/0014-4894(70)90103-7. [DOI] [PubMed] [Google Scholar]
  16. Shear H. L., Nussenzweig R. S., Bianco C. Immune phagocytosis in murine malaria. J Exp Med. 1979 Jun 1;149(6):1288–1298. doi: 10.1084/jem.149.6.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Territo M. C., Cline M. J. Monocyte function in man. J Immunol. 1977 Jan;118(1):187–192. [PubMed] [Google Scholar]
  18. Tosta C. E., Wedderburn N. Immune phagocytosis of Plasmodium yoelii-infected erythrocytes by macrophages and eosinophils. Clin Exp Immunol. 1980 Oct;42(1):114–120. [PMC free article] [PubMed] [Google Scholar]
  19. Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
  20. Trubowitz S., Masek B. Plasmodium falciparum: phagocytosis by polymorphonuclear leukocytes. Science. 1968 Oct 11;162(3850):273–274. doi: 10.1126/science.162.3850.273. [DOI] [PubMed] [Google Scholar]
  21. Vernes A. Phagocytosis of P falciparum parasitised erythrocytes by peripheral monocytes. Lancet. 1980 Dec 13;2(8207):1297–1298. doi: 10.1016/s0140-6736(80)92357-0. [DOI] [PubMed] [Google Scholar]
  22. Zucker-Franklin D. The percentage of monocytes among "mononuclear" cell fractions obtained from normal human blood. J Immunol. 1974 Jan;112(1):234–240. [PubMed] [Google Scholar]

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