Abstract
Pasteurella multocida serotype A was found in association with the mucosal epithelium of the nasopharynges of rabbits with respiratory tract infections. The bacteria specifically attached to squamous epithelial cells of the pharyngeal mucosa both in vivo and in vitro and to some tissue culture cell lines such as HeLa. All strains with serotype A capsules were adhesive. With the exception of one serotype D strain, strains with capsular serotypes B, D, and E were at least 10-fold less adhesive. Bacterial adhesiveness was much reduced after pronase digestion, heat treatment, and homogenization, but removal of the hyaluronic acid capsule increased adhesion. Electron microscopy revealed that fimbriae were produced by an adhesive pasteurella strain, but not by two nonadherent strains. The attachment of the former strain to pharyngeal and HeLa cells was inhibited by N-acetyl-D-glucosamine. Together, these findings suggest that this amino sugar may be a component of the receptor on both animal cell surfaces and that the fimbriae may be the adhesions. It is proposed that bacterial attachment has a role in colonization and infection of rabbit upper respiratory mucosae.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Carter G. R. Improved hemagglutination test for identifying type A strains of Pasteurella multocida. Appl Microbiol. 1972 Jul;24(1):162–163. doi: 10.1128/am.24.1.162-163.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carter G. R. Pasteurellosis: Pasteurella multocida and Pasteurella hemolytica. Adv Vet Sci. 1967;11:321–379. [PubMed] [Google Scholar]
- Collins F. M. Mechanisms of acquired resistance to Pasteurella multocida infection: a review. Cornell Vet. 1977 Jan;67(1):103–138. [PubMed] [Google Scholar]
- Hagen K. W. Enzootic pasteurellosis in domestic rabbits. II. Strain types and methods of control. Lab Anim Care. 1966 Dec;16(6):487–491. [PubMed] [Google Scholar]
- Henriksen S. D., Frøholm L. O. A fimbriated strain of Pasteurella multocida with spreading and corroding colonies. Acta Pathol Microbiol Scand B. 1975 Apr;83(2):129–132. doi: 10.1111/j.1699-0463.1975.tb00082.x. [DOI] [PubMed] [Google Scholar]
- Hofing G. L., Rush H. G., Petkus A. R., Glorioso J. C. In vitro killing of Pasteurella multocida: the effect of rabbit granulocyte and specific antibody source. Am J Vet Res. 1979 May;40(5):679–683. [PubMed] [Google Scholar]
- Hubald J., Erler W., Feist H., Flossmann K. D. Vorkommen von Mucopolysacchariden bei Pasteurella multocida: Isolierung von Hyaluronsäure aus A- und B-Serotyp-Stämmen. Z Allg Mikrobiol. 1981;21(1):41–45. [PubMed] [Google Scholar]
- Lu Y. S., Ringler D. H., Park J. S. Characterization of Pasteurella multocida isolates from the nares of healthy rabbits with pneumonia. Lab Anim Sci. 1978 Dec;28(6):691–697. [PubMed] [Google Scholar]
- Maheswaran S. K., Thies E. S. Influence of encapsulation on phagocytosis of Pasteurella multocida by bovine neutrophils. Infect Immun. 1979 Oct;26(1):76–81. doi: 10.1128/iai.26.1.76-81.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pestana de Castro A. F., Perreau P., Rodrigues A. C., Simoes M. Haemagglutinating properties of Pasteurella multocida type A strains isolated from rabbits and poultry. Ann Microbiol (Paris) 1980 May-Jun;131(3):255–263. [PubMed] [Google Scholar]
- Smith J. W., Glorioso J. C. Effect of cross-immunization on monotypic antibody responses to herpes simplex virus types 1 and 2. J Immunol. 1976 Apr;116(4):898–903. [PubMed] [Google Scholar]
- Watson W. T., Goldsboro J. A., Williams F. P., Sueur R. Experimental respiratory infection with Pasteurella multocida and Bordetella bronchiseptica in rabbits. Lab Anim Sci. 1975 Aug;25(4):459–464. [PubMed] [Google Scholar]