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. 1982 May;36(2):598–602. doi: 10.1128/iai.36.2.598-602.1982

Cytotoxic T cells are induced in mice infected with lymphocytic choriomeningitis virus strains of markedly different pathogenicities.

C J Pfau, J K Valenti, S Jacobson, D C Pevear
PMCID: PMC351270  PMID: 6979512

Abstract

The ability of two lymphocytic choriomeningitis virus substrains to induce cytotoxic T-lymphocyte (CTL) responses in intracerebrally infected mice was examined. One strain, designated A (aggressive), provoked a convulsive type of death in 100% of the mice within 8 to 9 days, whereas the other strain, designated D (docile), killed less than 10% of the mice during 28-day observation periods. CTL activity was assessed by the capacity of partially purified splenocytes to lyse 51Cr-labeled L-cell targets infected with either type of lymphocytic choriomeningitis substrain. The CTL population was identified by its sensitivity to anti-Thy-1 serum and its inability to lyse uninfected target cells or infected target cells with which it differed at the level of antigens controlled by the major histocompatibility gene complex. A strong CTL response developed in mice infected with either lymphocytic choriomeningitis substrain, although the activity provoked by substrain D was somewhat less than that seen after substrain A infection. Peak CTL activities induced by both strains occurred at about the same time. Even though docile virus replicated more extensively in the brain than did aggressive virus and fluorescent antibody staining revealed similar distributions of viral antigen, no inflammatory response was noted in the brains of mice infected with docile virus. These results are discussed with regard to the role of CTLs in mediating classic central nervous system pathology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buchmeier M. J., Welsh R. M., Dutko F. J., Oldstone M. B. The virology and immunobiology of lymphocytic choriomeningitis virus infection. Adv Immunol. 1980;30:275–331. doi: 10.1016/s0065-2776(08)60197-2. [DOI] [PubMed] [Google Scholar]
  2. Cihak J., Lehmann-Grube F. Immunological tolerance to lymphocytic choriomeningitis virus in neonatally infected virus carrier mice: evidence supporting a clonal inactivation mechanism. Immunology. 1978 Feb;34(2):265–275. [PMC free article] [PubMed] [Google Scholar]
  3. Cole G. A., Johnson E. D. Immune responses to LCM virus infection in vivo and in vitro. Mechanisms of immune-mediated disease. Bull World Health Organ. 1975;52(4-6):465–470. [PMC free article] [PubMed] [Google Scholar]
  4. Cole G. A., Nathanson N., Prendergast R. A. Requirement for theta-bearing cells in lymphocytic choriomeningitis virus-induced central nervous system disease. Nature. 1972 Aug 11;238(5363):335–337. doi: 10.1038/238335a0. [DOI] [PubMed] [Google Scholar]
  5. Doherty P. C., Zinernagel R. M. Capacity of sensitized thymus-derived lymphocytes to induce fatal lymphocytic choriomeningitis is restricted by the H-2 gene complex. J Immunol. 1975 Jan;114(1 Pt 1):30–33. [PubMed] [Google Scholar]
  6. Doherty P. C., Zinkernagel R. M. H-2 compatibility is required for T-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus. J Exp Med. 1975 Feb 1;141(2):502–507. doi: 10.1084/jem.141.2.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dutko F. J., Pfau C. J. Arenavirus defective interfering particles mask the cell-killing potential of standard virus. J Gen Virol. 1978 Feb;38(2):195–208. doi: 10.1099/0022-1317-38-2-195. [DOI] [PubMed] [Google Scholar]
  8. Gilden D. H., Cole G. A., Nathanson N. Immunopathogenesis of acute central nervous system disease produced by lymphocytic choriomeningitis virus. II. Adoptive immunization of virus carriers. J Exp Med. 1972 Apr 1;135(4):874–889. doi: 10.1084/jem.135.4.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hotchin J. Slow viruses and neurological damage. Monogr Hum Genet. 1972;6:172–181. doi: 10.1159/000392687. [DOI] [PubMed] [Google Scholar]
  10. Jacobson S., Friedman R. M., Pfau C. J. Interferon induction by lymphocytic choriomeningitis viruses correlates with maximum virulence. J Gen Virol. 1981 Dec;57(Pt 2):275–283. doi: 10.1099/0022-1317-57-2-275. [DOI] [PubMed] [Google Scholar]
  11. Jacobson S., Pfau C. J. Viral pathogenesis and resistance to defective interfering particles. Nature. 1980 Jan 17;283(5744):311–313. doi: 10.1038/283311a0. [DOI] [PubMed] [Google Scholar]
  12. Johnson E. D., Cole G. A. Functional heterogeneity of lymphocytic choriomeningitis virus-specfic T lymphocytes. I. Identification of effector amd memory subsets. J Exp Med. 1975 Apr 1;141(4):866–881. [PMC free article] [PubMed] [Google Scholar]
  13. Johnson E. D., Monjan A. A., Morse H. C., 3rd Lack of B-cell participation in acute lymphocyte choriomeningitis disease of the central nervous system. Cell Immunol. 1978 Mar 1;36(1):143–150. doi: 10.1016/0008-8749(78)90257-5. [DOI] [PubMed] [Google Scholar]
  14. Marker O., Volkert M. Studies on cell-mediated immunity to lymphocytic choriomeningitis virus in mice. J Exp Med. 1973 Jun 1;137(6):1511–1525. doi: 10.1084/jem.137.6.1511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morishima T., Hayashi K. Meningeal exudate cells in vaccinia meningitis of mice: role of local T cells. Infect Immun. 1978 Jun;20(3):752–759. doi: 10.1128/iai.20.3.752-759.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Suzuki S., Hotchin J. Initiation of persistent lymphocytic choriomeningitis infection in adult mice. J Infect Dis. 1971 Jun;123(6):603–610. doi: 10.1093/infdis/123.6.603. [DOI] [PubMed] [Google Scholar]
  17. Traub E. A FILTERABLE VIRUS RECOVERED FROM WHITE MICE. Science. 1935 Mar 22;81(2099):298–299. doi: 10.1126/science.81.2099.298. [DOI] [PubMed] [Google Scholar]
  18. VOLKERT M., LARSEN J. H., PFAU C. STUDIES ON IMMUNOLOGICAL TOLERANCE TO LCM VIRUS. 4. THE QUESTION OF IMMUNITY IN ADOPTIVELY IMMUNIZED VIRUS CARRIERS. Acta Pathol Microbiol Scand. 1964;61:268–282. doi: 10.1111/apm.1964.61.2.268. [DOI] [PubMed] [Google Scholar]
  19. Welsh R. M., Oldstone M. B. Inhibition of immunologic injury of cultured cells infected with lymphocytic choriomeningitis virus: role of defective interfering virus in regulating viral antigenic expression. J Exp Med. 1977 Jun 1;145(6):1449–1468. doi: 10.1084/jem.145.6.1449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Welsh R. M., Trowbridge R. S., Kowalski J. B., O'Connell C. M., Peau C. J. Amantadine hydrochloride inhibition of early and late stages of lymphocytic choriomenigitis virus-cell interactions. Virology. 1971 Sep;45(3):679–686. [PubMed] [Google Scholar]
  21. Zinkernagel R. M., Doherty P. C. Cytotoxic thymus-derived lymphocytes in cerebrospinal fluid of mice with lymphocytic choriomeningitis. J Exp Med. 1973 Nov 1;138(5):1266–1269. doi: 10.1084/jem.138.5.1266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zinkernagel R. M., Doherty P. C. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv Immunol. 1979;27:51–177. doi: 10.1016/s0065-2776(08)60262-x. [DOI] [PubMed] [Google Scholar]

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