Abstract
Centrifugation enhanced the infectivity of invasive Escherichia coli and Yersinia enterocolitica for HEp-2 cells. Noninvasive bacteria were not endocytosed after centrifugation. The centrifugation procedure may increase the sensitivity of testing for bacterial invasiveness in cell culture without causing false-positive results.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Darougar S., Cubitt S., Jones B. R. Effect of high-speed centrifugation on the sensitivity of irradiated McCoy cell culture for the isolation of Chlamydia. Br J Vener Dis. 1974 Aug;50(4):308–312. doi: 10.1136/sti.50.4.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Devenish J. A., Schiemann D. A. HeLa cell infection by Yersinia enterocolitica: evidence for lack of intracellular multiplication and development of a new procedure for quantitative expression of infectivity. Infect Immun. 1981 Apr;32(1):48–55. doi: 10.1128/iai.32.1.48-55.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DuPont H. L., Formal S. B., Hornick R. B., Snyder M. J., Libonati J. P., Sheahan D. G., LaBrec E. H., Kalas J. P. Pathogenesis of Escherichia coli diarrhea. N Engl J Med. 1971 Jul 1;285(1):1–9. doi: 10.1056/NEJM197107012850101. [DOI] [PubMed] [Google Scholar]
- Giannella R. A., Washington O., Gemski P., Formal S. B. Invasion of HeLa cells by Salmonella typhimurium: a model for study of invasiveness of Salmonella. J Infect Dis. 1973 Jul;128(1):69–75. doi: 10.1093/infdis/128.1.69. [DOI] [PubMed] [Google Scholar]
- Hale T. L., Formal S. B. Protein synthesis in HeLa or Henle 407 cells infected with Shigella dysenteriae 1, Shigella flexneri 2a, or Salmonella typhimurium W118. Infect Immun. 1981 Apr;32(1):137–144. doi: 10.1128/iai.32.1.137-144.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harris J. C., Dupont H. L., Hornick R. B. Fecal leukocytes in diarrheal illness. Ann Intern Med. 1972 May;76(5):697–703. doi: 10.7326/0003-4819-76-5-697. [DOI] [PubMed] [Google Scholar]
- Kapperud G. Studies on the pathogenicity of Yersinia enterocolitica and Y. enterocolitica-like bacteria. 2. Interaction with HeLa cells among environmental and human isolates from Scandinavia. Acta Pathol Microbiol Scand B. 1980 Oct;88(5):293–297. [PubMed] [Google Scholar]
- Labrec E. H., Schneider H., Magnani T. J., Formal S. B. EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY. J Bacteriol. 1964 Nov;88(5):1503–1518. doi: 10.1128/jb.88.5.1503-1518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee W. H., McGrath P. P., Carter P. H., Eide E. L. The ability of some Yersinia enterocolitica strains to invade HeLa cells. Can J Microbiol. 1977 Dec;23(12):1714–1722. doi: 10.1139/m77-247. [DOI] [PubMed] [Google Scholar]
- Mehlman I. J., Eide E. L., Sanders A. C., Fishbein M., Aulisio C. C. Methodology for recognition of invasive potential of Escherichia coli. J Assoc Off Anal Chem. 1977 May;60(3):546–562. [PubMed] [Google Scholar]
- Mäki M., Grönroos P., Vesikari T. In vitro invasiveness of Yersinia enterocolitica isolated from children with diarrhea. J Infect Dis. 1978 Nov;138(5):677–680. doi: 10.1093/infdis/138.5.677. [DOI] [PubMed] [Google Scholar]
- Mäki M., Vesikari T., Grönroos P. Enterotoxigenic and invasive Escherichia coli as causes of childhood diarrhoea in Finland. Acta Paediatr Scand. 1980 Mar;69(2):219–224. doi: 10.1111/j.1651-2227.1980.tb07064.x. [DOI] [PubMed] [Google Scholar]
- Mäki M., Vesikari T., Rantala I., Grönroos P. Yersiniosis in children. Arch Dis Child. 1980 Nov;55(11):861–865. doi: 10.1136/adc.55.11.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pedersen K. B., Winblad S., Bitsch V. Studies on the interaction between different O-serotypes of Yersinia enterocolitica and HeLa cells. Acta Pathol Microbiol Scand B. 1979 Apr;87B(2):141–145. doi: 10.1111/j.1699-0463.1979.tb02417.x. [DOI] [PubMed] [Google Scholar]
- Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rudoy R. C. Enteroinvasive and enterotoxigenic Escherichia coli. Occurrence in acute diarrhea of infants and children. Am J Dis Child. 1975 Jun;129(6):668–672. doi: 10.1001/archpedi.1975.02120430008004. [DOI] [PubMed] [Google Scholar]
- SERENY B. Experimental shigella keratoconjunctivitis; a preliminary report. Acta Microbiol Acad Sci Hung. 1955;2(3):293–296. [PubMed] [Google Scholar]
- Une T. Studies on the pathogenicity of Yersinia enterocolitica. II. Interaction with cultured cells in vitro. Microbiol Immunol. 1977;21(7):365–377. doi: 10.1111/j.1348-0421.1977.tb00301.x. [DOI] [PubMed] [Google Scholar]
- Vesikari T., Nurmi T., Mäki M., Skurnik M., Sundqvist C., Granfors K., Grönroos P. Plasmids in Yersinia enterocolitica serotypes O:3 and O:9: correlation with epithelial cell adherence in vitro. Infect Immun. 1981 Sep;33(3):870–876. doi: 10.1128/iai.33.3.870-876.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]