Abstract
The results of four studies described in this paper support the idea that pneumococcal autolysin (N-acetylmuramyl-L-alanine amidase) plays a key role in generating the pneumococcal purpura-producing principle (PPP) from the bacterial cell wall. This conclusion is based on the observation that (i) concentrates of pneumococcal cell-free extracts prepared at a pH which is inhibitory to autolysin activity contained significantly less PPP activity than extract concentrates prepared at a pH which is optimal for autolysin activity; (ii) PPP activity was not detectable in concentrates of cell-free extracts prepared from cells which contained ethanolamine in the cell wall, but a normal amount of activity was found in extract concentrates derived from cells containing choline in the cell wall; (iii) PPP activity was not detectable in concentrates of cell-free extracts prepared from an autolysin-defective mutant, but a normal amount of activity was found in extract concentrates derived from the wild-type strains; and (iv) incubation of purified wild-type strain cell walls with pneumococcal autolysin resulted in a significant increase in the amount of purpurogenic activity in the supernatant fluids of the incubation mixtures.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blumenkrantz N., Asboe-Hansen G. An assay for total hexosamine and a differential assay for glucosamine and galactosamine. Clin Biochem. 1976 Dec;9(6):269–274. doi: 10.1016/s0009-9120(76)80075-6. [DOI] [PubMed] [Google Scholar]
- Chetty C., Kreger A. Characterization of pneumococcal purpura-producing principle. Infect Immun. 1980 Jul;29(1):158–164. doi: 10.1128/iai.29.1.158-164.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Höltje J. V., Tomasz A. Purification of the pneumococcal N-acetylmuramyl-L-alanine amidase to biochemical homogeneity. J Biol Chem. 1976 Jul 25;251(14):4199–4207. [PubMed] [Google Scholar]
- Höltje J. V., Tomasz A. Teichoic acid phosphorylcholine esterase. A novel enzyme activity in pneumococcus. J Biol Chem. 1974 Nov 10;249(21):7032–7034. [PubMed] [Google Scholar]
- Koga T., Maeda K., Onoue K., Kato K., Kotani S. Chemical structure required for immunoadjuvant and arthritogenic activities of cell wall peptidoglycans. Mol Immunol. 1979 Mar;16(3):153–162. doi: 10.1016/0161-5890(79)90140-8. [DOI] [PubMed] [Google Scholar]
- Kohashi O., Pearson C. M., Watanabe Y., Kotani S. Preparation of arthritogenic hydrosoluble peptidoglycans from both arthritogenic and non-arthritogenic bacterial cell walls. Infect Immun. 1977 Jun;16(3):861–866. doi: 10.1128/iai.16.3.861-866.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LACKS S., HOTCHKISS R. D. A study of the genetic material determining an enzyme in Pneumococcus. Biochim Biophys Acta. 1960 Apr 22;39:508–518. doi: 10.1016/0006-3002(60)90205-5. [DOI] [PubMed] [Google Scholar]
- Lacks S. Mutants of Diplococcus pneumoniae that lack deoxyribonucleases and other activities possibly pertinent to genetic transformation. J Bacteriol. 1970 Feb;101(2):373–383. doi: 10.1128/jb.101.2.373-383.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mcdonnell M., Lain R., Tomasz A. "Diplophage": a bacteriophage of Diplococcus pneumoniae. Virology. 1975 Feb;63(2):577–582. doi: 10.1016/0042-6822(75)90329-3. [DOI] [PubMed] [Google Scholar]
- Mosser J. L., Tomasz A. Choline-containing teichoic acid as a structural component of pneumococcal cell wall and its role in sensitivity to lysis by an autolytic enzyme. J Biol Chem. 1970 Jan 25;245(2):287–298. [PubMed] [Google Scholar]
- Rane L., Subbarow Y. Nutritional Requirements of the Pneumococcus: I. Growth Factors for Types I, II, V, VII, VIII. J Bacteriol. 1940 Nov;40(5):695–704. doi: 10.1128/jb.40.5.695-704.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ronda-Lain C., Lopez R., Tapia A., Tomasz A. Role of the pneumococcal autolysin (murein hydrolase) in the release of progeny bacteriophage and in the bacteriophage-induced lysis of the host cells. J Virol. 1977 Jan;21(1):366–374. doi: 10.1128/jvi.21.1.366-374.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rotta J., Rýc M., Masek K., Zaoral M. Biological activity of synthetic subunits of streptococcus peptidoglycan. I. Pyrogenic and thrombocytolytic activity. Exp Cell Biol. 1979;47(4):258–268. doi: 10.1159/000162944. [DOI] [PubMed] [Google Scholar]
- SMITS G. Modification of the periodide method for the determination of choline. Biochim Biophys Acta. 1957 Nov;26(2):424–427. doi: 10.1016/0006-3002(57)90026-4. [DOI] [PubMed] [Google Scholar]
- Seto H., Tomasz A. Protoplast formation and leakage of intramembrane cell components: induction by the competence activator substance of pneumococci. J Bacteriol. 1975 Jan;121(1):344–353. doi: 10.1128/jb.121.1.344-353.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomasz A., Albino A., Zanati E. Multiple antibiotic resistance in a bacterium with suppressed autolytic system. Nature. 1970 Jul 11;227(5254):138–140. doi: 10.1038/227138a0. [DOI] [PubMed] [Google Scholar]
- Tomasz A. Biological consequences of the replacement of choline by ethanolamine in the cell wall of Pneumococcus: chanin formation, loss of transformability, and loss of autolysis. Proc Natl Acad Sci U S A. 1968 Jan;59(1):86–93. doi: 10.1073/pnas.59.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomasz A., Westphal M. Abnormal autolytic enzyme in a pneumococus with altered teichoic acid composition. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2627–2630. doi: 10.1073/pnas.68.11.2627. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomasz A., Westphal M., Briles E. B., Fletcher P. On the physiological functions of teichoic acids. J Supramol Struct. 1975;3(1):1–16. doi: 10.1002/jss.400030102. [DOI] [PubMed] [Google Scholar]