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. 1978 May;13(5):854–860. doi: 10.1128/aac.13.5.854

Antibiotic Susceptibility of Clinical Isolates of Listeria monocytogenes

Geraldine L Wiggins 1, William L Albritton 1, John C Feeley 1
PMCID: PMC352344  PMID: 96737

Abstract

A broth microdilution method was used to measure the minimal inhibitory concentrations (MICs) of the antibiotics most often recommended for treatment of listeriosis. The MICs of ampicillin, penicillin, erythromycin, and tetracycline for 175 strains of Listeria monocytogenes were below the approximate MIC breakpoint for susceptible strains as recommended by the National Committee on Clinical Laboratory Standards. Inhibition diameters for 125 strains were measured by the standardized disk method (National Committee on Clinical Laboratory Standards) and compared with the appropriate MIC values. By both methods, strains were susceptible to the above four antibiotics, except for three strains, which were intermediate in susceptibility to penicillin by the disk method. Since the minimal bactericidal concentrations for ampicillin and penicillin significantly exceeded the MICs for these antibiotics, 45 strains were evaluated with ampicillin (5 μg/ml) and gentamicin (1 μg/ml) to compare the synergistic bactericidal effect of the two used in combination and singly. An increased kill of 100-fold was observed with the antibiotics combined in 19 strains after 4 to 6 h and in 40 strains after 24 h. A comparison of results with microdilution in Trypticase soy broth and agar dilution in Mueller-Hinton agar revealed that MICs for gentamicin, kanamycin, and streptomycin were strongly influenced by the media used. The MICs were consistently lower in Mueller-Hinton agar.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BINDER M. A., DIEHL C., WEISS J., RAY H. Listeria meningitis. Ann Intern Med. 1953 Jun;38(6):1315–1319. doi: 10.7326/0003-4819-38-6-1315. [DOI] [PubMed] [Google Scholar]
  2. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  3. Bojsen-Moller J. Human listeriosis. Diagnostic, epidemiological and clinical studies. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;Suppl 229:1–157. [PubMed] [Google Scholar]
  4. Buchner L. H., Schneierson S. Clinical and laboratory aspects of Listeria monocytogenes infections. With a report of ten cases. Am J Med. 1968 Dec;45(6):904–921. doi: 10.1016/0002-9343(68)90189-7. [DOI] [PubMed] [Google Scholar]
  5. D'amato R. F., Thornsberry C., Baker C. N., Kirven L. A. Effect of calcium and magnesium ions on the susceptibility of Pseudomonas species to tetracycline, gentamicin polymyxin B, and carbenicillin. Antimicrob Agents Chemother. 1975 May;7(5):596–600. doi: 10.1128/aac.7.5.596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gordon R. C., Barrett F. F., Clark D. J. Influence of several antibiotics, singly and in combination, on the growth of Listeria monocytogenes. J Pediatr. 1972 Apr;80(4):667–670. doi: 10.1016/s0022-3476(72)80072-6. [DOI] [PubMed] [Google Scholar]
  7. Gordon R. C., Barrett F. F., Yow M. D. Ampicillin treatment of listeriosis. J Pediatr. 1970 Dec;77(6):1067–1070. doi: 10.1016/s0022-3476(70)80098-1. [DOI] [PubMed] [Google Scholar]
  8. HOEPRICH P. D. Infection due to Listeria monocytogenes. Medicine (Baltimore) 1958 May;37(2):143–160. doi: 10.1097/00005792-195805000-00002. [DOI] [PubMed] [Google Scholar]
  9. HOOD M. Listeriosis; report of 10 cases. Am J Clin Pathol. 1957 Jul;28(1):18–26. doi: 10.1093/ajcp/28.1.18. [DOI] [PubMed] [Google Scholar]
  10. Lavetter A., Leedom J. M., Mathies A. W., Jr, Ivler D., Wehrle P. F. Meningitis due to Listeria monocytogenes. A review of 25 cases. N Engl J Med. 1971 Sep 9;285(11):598–603. doi: 10.1056/NEJM197109092851103. [DOI] [PubMed] [Google Scholar]
  11. McCracken G. H., Jr, Nelson J. D., Thomas M. L. Discrepancy between carbenicillin and ampicillin activities against enterococci and Listeria. Antimicrob Agents Chemother. 1973 Mar;3(3):343–349. doi: 10.1128/aac.3.3.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Medoff G., Kunz L. J., Weinberg A. N. Listeriosis in humans: an evaluation. J Infect Dis. 1971 Mar;123(3):247–250. doi: 10.1093/infdis/123.3.247. [DOI] [PubMed] [Google Scholar]
  13. Moellering R. C., Jr, Medoff G., Leech I., Wennersten C., Kunz L. J. Antibiotic synergism against Listeria monocytogenes. Antimicrob Agents Chemother. 1972 Jan;1(1):30–34. doi: 10.1128/aac.1.1.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NYSTROM K. G., KARLSSON K. A. Sensitivity of Listeria monocytogenes in vitro to different antibiotics and chemotherapeutics. Acta Paediatr. 1961 Mar;50:113–116. doi: 10.1111/j.1651-2227.1961.tb08029.x. [DOI] [PubMed] [Google Scholar]
  15. Nelson J. D., Shelton S., Parks D. Antibiotic susceptibility of Listeria monocytogenes and treatment of neonatal listeriosis with ampicillin. Acta Paediatr Scand. 1967 Mar;56(2):151–158. doi: 10.1111/j.1651-2227.1967.tb15358.x. [DOI] [PubMed] [Google Scholar]
  16. Reller L. B., Schoenknecht F. D., Kenny M. A., Sherris J. C. Antibiotic susceptibility testing of Pseudomonas aeruginosa: selection of a control strain and criteria for magnesium and calcium content in media. J Infect Dis. 1974 Nov;130(5):454–463. doi: 10.1093/infdis/130.5.454. [DOI] [PubMed] [Google Scholar]
  17. SEPP A. H., ROY T. E. Listeria monocytogenes infections in Metropolitan Toronto. A clinicopathological study. Can Med Assoc J. 1963 Mar 16;88:549–561. [PMC free article] [PubMed] [Google Scholar]
  18. Thornsberry C., Baker C. N., Facklam R. R. Antibiotic susceptibility of Streptococcus bovis and other group D streptococci causing endocarditis. Antimicrob Agents Chemother. 1974 Mar;5(3):228–233. doi: 10.1128/aac.5.3.228. [DOI] [PMC free article] [PubMed] [Google Scholar]

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